Phytochemistry 42, 857-762 (1996). TLC of new proanthocyanidins on silica with ethyl acetate - formic acid - water 18:1:1. Detection with vanillin - HCl reagent of 1 % ethanolic FeCl3 solution. Isolation of acetylated derivatives by prep. TLC on silica with toluene - acetone 7:3.

Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 363-371. HPTLC of selected flavan-3-ols [(-)-epicatechin-3-gallate, (+)-catechin, (-)-epicatechin and (-)-epigallocatechin] on cellulose with aqueous solutions of sodium chloride (10 mg/ml) containing different concentrations of b-cyclodextrin. Acetone and 1-butanol were used as organic modifiers. An unsaturated horizontal developing chamber was used for the separations. Detection under UV 366nm. After that the spots were visualized by dipping the plates in anisaldehyde-sulfuric acid reagent. A Video Documentation System was used for recording and analyzing the images of the developed TLC plates.

Planta med. 66, 403-407 (2000). Preparative TLC of machilusol A, B, C, D, E, and F on silica gel with n-hexane - acetone 1:1, dichloromethane - acetone 10:1 and dichloromethane - ethyl acetate 10:1. Visualization by spraying with cerous sulfate solution, detection by heating.

J. Chromatogr. A 1077 (2), 188-194 (2005). HPTLC of (+)-catechin (C), (-)-epicatechin (EC), (-)-gallocatechin (GC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECg), (-)-epigallocatechin gallate (EGCg), procyanidin B1, and procyanidin B2 on cellulose prewashed with water (not necessary, when water was used as developing solvent) and dried with a hair dryer, with 1) water; 2) 1-propanol - water 1:4; 3) 1-propanol - water - acetic acid 4:2:1; 4) 1-propanol - water - acetic acid 2:8:1 in horizontal developing chamber (sandwich configuration). Detection with vanillin - H3PO4 reagent. Water enabled the separation of epimers C from EC and GC from EGC, as well as the dimers procyanidin B1 and B2. Additionally C, EGC, B1 and B2 were separated from all the other compounds. The best separation of the five main catechins (EC, GC, EGC, ECg, EGCg) present in green tea extract was achieved using 1-propanol - water - acetic acid 2:8:1. The chromatograms of oak bark extract developed in solvents with higher water content (1-propanol - water 1:4 and 1-propanol - water - acetic acid 2:8:1) showed less bands than chromatograms developed in solvents with higher organic modifier content (e.g. 1-propanol - water - acetic acid 4:2:1). It was proved that such behavior was due to the presence of procyanidins beside the main component catechin.

Phytochemistry 22, 1520-1521 (1983). TLC of flavone and flavanone aglycones on microcrystalline cellulose with 50 % acetic acid, on polyamide with toluene-MEK - acetic acid 4:3:3 and on silica with chloroform - methanol - water - acetic acid 100:15:0.5:0.3. Detection by UV. Excellent resolution of closely related flavonoid aglycones.

J. Agric. Food Chem. 33, 745-746 (1985). TLC of deoxynivalenol and 15-acetyldeoxynivalenol on silica with toluene - ethyl acetate 1:3, detection by spraying with 30 % sulfuric acid and heating at 110 °C for 5 min., or spraying with 15 % AlCl3 solution in ethanol - water 85:15 and subsequent UV visualization. Assay by visual comparison with standard.

J. Agric. Food Chem. 38, 1194-1197 (1990). Preparative TLC of tanshinone on silica with petrol ether - ether - acetone 75:20:5.

J. Planar Chromatogr. 6, 223-227 (1993). HPTLC of flavone, 5-methoxy-isoflavone, and ipriflavone (7-isopropoxyisoflavone) on silica with hexane - ether 6:4; two-dimensional TLC on silica with hexane - ether 3:1 (first direction), same solvent (second direction) and chloroform - ethyl acetate 95:5 (again first direction). Detection with iodine/potassium iodide and 1 % uranyl acetate as spray reagents; charring by spraying with 7 mL of 25 % sulfuric acid and heating at 250 °C for 1 min. Quantification by densitometry in absorbance or fluorescence mode.