Lebensm. Unters. Forsch. 175, 337-341 (1982). Diterpenoide im Kaffee. I. (Diterpenes in coffee. I. Atractyligenin.) TLC of atractyligenin on silica with chloroform - methanol 85:15. Detection by dipping into a solution of 1 ml anisaldehyde + 2 ml sulfuric acid + 100 ml acetic acid. Densitometry at 546 nm.

Chromatogr. 287, 419- 424 (1984). (Microspectrofluorometric dosage on TLC of monomethyihydrazine of Gyromitra esculenta.) TLC of monomethylhydrazine on cellulose with butanol-ethanol - water 4:1:2. Detection by UV after spraying with 0.5 % p-dimethylaninocinnamaldehyde in ethanol, containing 4 % sulfuric acid and then water.

TLC in pharmacy: chamomile leaf. Deutsche Apotheker Zeitung 131, 2616-2619 (1991). TLC of borneol and bornylacetate on silica with toluene – ethyl acetate 93:7. Detection by spraying with anisaldehyde – sulfuric acid reagent and under UV 254 and 366 nm.

J. Agric. Food Chem. 48, 231-234 (2000). TLC of the unsaponifiables (hydrocarbons, triterpene alcohols, sterols) on silica gel, bandwise applied, with hexane - ethyl acetate 6:1. Detection after spraying with 0.5% Rhodamine 6G solution and observation under UV. After elution and extraction silanation of sterols and triterpene alcohols with BSTFA for GC investigation.

Journal of Natural Remedies 8(1), 61-67 (2008). During pharmacognostic studies on Sphaeranthus indicus (Asteraceae) the sesquiterpenoid 7-OH-eudesmanolide was isolated. HPTLC of the ethyl acetate soluble fraction of aqueous alcoholic Sphaeranthus indicus extracts, on silica gel with n-hexane - diethyl ether 3:7. The plant extract and the marker (7-OH-eudesmanolide) were chromatographed simultaneously. Quantitative determination by absorbance measurement at 213 nm.

J. AOAC Int. 96, 1209-1213 (2013). TLC of thujone (1) and 1,8-cineole (2) in the leaves of Artemisia adamsii essential oil on silica gel with toluene - ethyl acetate 93:7. Detection by dipping into vanillin - sulfuric acid reagent, followed by heating at 90 ºC for 3 min. The hRf values for (1) and (2) were 56 and 45. Bioautography by dipping into S. aureus suspension for 10 s. After drying for 2 min followed by incubation at 37 ºC for 17 h the plates were dipped in an aqueous solution of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide 50 mg/80 mL) for 10 s and incubated at 37°C for 2 h. Inhibition zones appeared as pale yellowish zones against blue background.

J. Chromatogr. A 1530, 197-203 (2017). Evaluation of the phenolic and stigmasterol content and comparison of the antioxidant and antidiabetic activities by HPTLC combined with DPPH* free radical method and α-amylase assay towards ethanol and ethyl acetate extracts of 10 marine macroalgae species (3 chlorophyta, 4 phaeophyta and 3 rhodophyta). HPTLC on silica gel with n-hexane – ethyl acetate – acetic acid 20:9:1 over 80 mm. Detection either by dipping into 0.4 % DPPH* solution followed by storing in the dark for 30 min before evaluation, or by dipping into anisaldehyde – sulfuric acid reagent or neutralized ferric chloride solution (prepared by adding dilute sodium hydroxide to freshly prepared 2 % ferric chloride in methanol until precipitation occurs, followed by filtering), both followed by heating at 100 °C for 10 min. Quantitative evaluation by absorbance measurement at 470 nm for detection of α-amylase inhibition and at 550 nm for stigmasterol. The results showed higher antioxidant activity in the ethyl acetate extracts than in ethanol extracts, and higher amounts of fucoxanthin, stigmasterol and α-amylase inhibitory activities in ethyl acetate extracts. The results proved the relation of higher α-amylase inhibition in ethyl acetate extracts due to the presence of triterpenes and sterols, and no contribution of fucoxanthin to it.

Virgaureasaponin 1, a new bisdesmosidic triterpenoid saponin of Solidago virgaurea L. Pharmazie 42, 541-543 (1987). TLC of saponins on silica with chloroform - methanol - water 15:10:2, with chloroform - methanol 9:1 and with n-butanol - ethanol - water 4:1:5 (upper phase). Detection reagents: blood gelatin for saponins, anisaldehyde - sulfuric acid for saponins and sapogenins and thymol - sulfuric acid for sugars. A new triterpenesaponin isolated and identified.