J. Chromatogr. A 1530, 197-203 (2017). Evaluation of the phenolic and stigmasterol content and comparison of the antioxidant and antidiabetic activities by HPTLC combined with DPPH* free radical method and α-amylase assay towards ethanol and ethyl acetate extracts of 10 marine macroalgae species (3 chlorophyta, 4 phaeophyta and 3 rhodophyta). HPTLC on silica gel with n-hexane – ethyl acetate – acetic acid 20:9:1 over 80 mm. Detection either by dipping into 0.4 % DPPH* solution followed by storing in the dark for 30 min before evaluation, or by dipping into anisaldehyde – sulfuric acid reagent or neutralized ferric chloride solution (prepared by adding dilute sodium hydroxide to freshly prepared 2 % ferric chloride in methanol until precipitation occurs, followed by filtering), both followed by heating at 100 °C for 10 min. Quantitative evaluation by absorbance measurement at 470 nm for detection of α-amylase inhibition and at 550 nm for stigmasterol. The results showed higher antioxidant activity in the ethyl acetate extracts than in ethanol extracts, and higher amounts of fucoxanthin, stigmasterol and α-amylase inhibitory activities in ethyl acetate extracts. The results proved the relation of higher α-amylase inhibition in ethyl acetate extracts due to the presence of triterpenes and sterols, and no contribution of fucoxanthin to it.

Herba Hungarica 26, 159-170 (1987). TLC of sesquiterpene lactone on silica with benzene - n-hexane - acetonitrile 30:20:21 and carbon tetrachloride - acetonitrile 40:1.5. Detection by spraying with a) 2% sulfuric acid in ethanol, heating at 110°C for 2-3 min, and b) phosphomolybdic acid and heating at 100°C for 2 min. OPTLC on silica with 1. carbon tetrachloride - acetonitrile 4:1.5, 2. n-hexane - ethyl acetate 2:1, 3. n-hexane - ethyl acetate 7:3, 4. n-hexane - acetonitrile - benzene 20:21:30. Densitometry by absorbance.

J. of Natural Products 51, 509-512 (1988). TLC of four new guaianes and four isocedrene derivatives on silica with ether - petrol ether 3:1 or chloroform - benzene - ethyl acetate 1:1:1.

Phytochemistry 28, 425-430 (1989). TLC of rugosal A and rugosic acid A on silica with hexane - ethyl acetate 3:1. Detection under UV 254 nm or by spraying with vanil- lin - sulfuric acid or phosphomolybdic acid reagents.

for high artemisin yielding types. Phytochemical Analysis 2, 80-83 (1991). TLC of Artemisia annua toluene extracts on silica with petrol ether - ether 1:1. Detection of the antimalarial compound artemisinin by immersion of the plate in a freshly prepared mixture of 50 mL acetic acid , 1 mL conc. H2SO4 and 0.5 mL anisaldehyde. Artemisinin occurs purple-red coloured (Rf = 0.43). Semi-quantitative determination by visual estimation.

Biochemical Systematics and Ecology 24, 37-42 (1996). TLC of sesquiterpenes on silica with butanol - acetone - water 3:1:1.

Phytochemistry 51, 429-433 (1999). TLC of new acetogenin derivatives on silica with chloroform - methanol 10:1, and ethyl acetate - acetone 15:1. Detection with Kedde's reagent. Isolation of closely related acetogenins by preparative TLC with the same solvent systems.

Juss. J. Planar Chromatogr. 16, 311-314 (2003). HPTLC of azadirachtin on silica gel with toluene - ethyl acetate - formic acid 10:8:1 with chamber saturation for 20 min. at 25°C. After drying of the plate visualization by spraying with vanillin-sulfuric acid reagent (3% vanillin in 1% ethanolic sulfuric acid) and heating at 100°C for 2 min. Quantitation by densitometry at 677 nm. Development of a simple, precise, and specific method.