Acta Alimentaria 21, 131-135 (1992). TLC of seed oil and its methyl esters on silica with hexane - ether - acetic acid 79:20:1. Visualization by spraying with conc. sulfuric acid. The methyl esters of castor and sal seed oil were used for reference.

J. Chromatogr. A 776, 355-360 (1997). Test of the efficiency of 11 TLC systems for the separation of chamomile. Use of the information theory and the methods of numerical taxonomy. Discussion of the design of the most effective series of TLC system in terms of discriminating power and mean information content, having silica gel layer and chloroform - toluene 8:1 and chloroform - toluene - ethyl acetate 13:6:1 as the most favorable systems. Identification of an unknown component using minimum number of system.

J. Planar Chromatogr. 23, 293-297 (2010). TLC of eugenol and piperine on silica gel with toluene - ethyl acetate 9:3 with chamber saturation for 15 min at room temperature. The hRf value of eugenol was 73 and of piperine 28. Linearity was between 1 and 12 µg/band for both eugenol and piperine. A good linear relationship with r2 = 0.9979 and 0.9980 for eugenol and piperine was obtained. The limit of detection was 0.1 ng/band for eugenol and 10 ng/band for piperine. Recovery of eugenol and piperine was above 97 %. The recovery (measured at different concentration levels) of eugenol was 95.9- 97.5 % and of piperine 91.8-96.6 %. For eugenol the robustness of the method (% RSD, n = 3) was 0.74 % (12 ng/band) and 0.69 % (25 ng/band) for toluene - ethyl acetete 9:3 and 0.79 % (12 ng/band) and 0.88 % (25 ng/band) for toluene - ethyl acetate 9:2. For piperine the robustness of the method (% RSD, n = 3) was 0.74 % (5 ng/band) and 0.89 % (10 ng/band) for toluene - ethyl acetate 9:3 and 0.72 % (5 ng/band) and 0.73 % (10 ng/band) for toluene - ethyl acetate 9:2.

46, 99-104 (1982). Triterpene un Steroide in Samen von Solanum dulcamara. (Triterpenes and steroids in seeds of solanum dulcamara.) TLC of triterpenes, sterols, spirosolanol glycosides on silica and AgNO3 treated silica with 9 different solvent systems, e.g. petrol ether - ethyl acetate - toluene 80:15:5, hexane - benzene 1:1, chloroform - methanol -1 % NH3 2:2:1. Detection with molybdo-H3PO4) reagent, anisaldehyde - sulfuric acid, Ehrlich's reagent, Dragendorff reagent, Clarke-reagent.

(Hungarian). Acta Pharm.Hungarica 57, 119-132 (1987). TLC methods recommended for medicinal plant analysis: (All separations on silica) Volatile oils with benzene - ethyl acetate 95:5 or with hexane; spraying with 3% vanillin + 3% sulfuric acid in ethanol. Separation of flavonoids with ethyl acetate - formic acid - acetic acid - water 100:11:11:26, flavone glucosides with ethyl acetate - formic acid - water 10:2:1, flavone aglucones with toluene - ethyl formiate - formic acid 5:4:1; detection under UV after spraying with methanolic aluminium chloride solution. Two-dimensional techniques for anthraquinones: ethyl acetate - methanol - water 200:33:27 (first dimension), toluene - ethyl acetate - formic acid 10:8:1 (second), detection under UV 366 nm. Separation of organic acids with chloroform - ethyl acetate - formic acid 3:2:2; detection under UV 366 nm after spraying with 3% FeCl3 in methanol.

Phytochemistry 48, 165-169 (1998). TLC of furano-diterpenes on silica gel with toluene- ethyl acetate - methanol 40:9:1. Detection under UV 254 and by spraying with a 1% solution of 4-dimethylaminobenzaldehyde. Isolation from a crude dichloromethane extract by preparative TLC on silica gel with hexane - ethyl acetate 7:3 and 1:1.

Planta med. 66, 487-489 (2000). Preparative TLC of hanliuine I and II on silica gel with n-hexane - acetone 2:3 and chloroform - hexane - methanol 6:1:1. Visualization under UV.

Asian Journal of Plant Sciences 9(1), 44-50 (2010). Beta-sitosterol is one of the major phytoconstituents in cornsilk (style and stigma of Zea mays Linn.). An HPTLC method for the standardization of cornsilk as a bioavailable source of beta-sitosterol is reported. HPTLC of methanolic extracts of dried cornsilk and plasma of rabbits (1 h after injection of plant slurry) on silica gel with toluene - ethyl acetate - methanol - glacial acetic acid 80:10:5:3 with chamber saturation for 30 min. Detection by treatment with Liebermann-Burchard reagent. Beta-sitosterol was well resolved with an hRf value of 48. Quantitative determination by fluorescence measurement at 366 nm. The method was suitable for the pharmacokinetic study (absorption - elimination). The study confirmed the bioavailability of beta-sitosterol from cornsilk, which is therefore a potential natural source of beta-sitosterol.