Anal. Biochem. 270, 314-422 (1999). HPTLC of the dihexadecyl phosphatidylethanolamine (DHPE) derivatives of the periodate oxidation products of disaccharides, and of oligosaccharide alditols, respectively, on silica gel with chloroform ñ methanol - water 130:50:9. Visualization after spraying with primuline reagent under long-wave UV. Quantitative estimates by fluorometric scanning densitometry. In situ analysis of the derivatives by liquid secondary ion mass spectrometry (LSIMS).

J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (2), 122-124 (2001). TLC on silica gel with 1) chloroform - methanol 17:3, 2) ethyl acetate - methanol 9:1. Detection by spraying with 10% sulfuric acid in ethanol and heating at 105°Cfor 5 min. Identification by finger print technique. Quantitation of polysaccharides by UV spectroscopy.

J. Planar Chromatogr. 17, 420-423 (2004). A new on-line TLC-MS interface, with computer-controlled extraction of substances from selected spots on a TLC or HPTLC plate, has been constructed. The controlled collection of the sample and its programmed injection into the mass spectrometer is the advantage of this type of interface. It has been tested and validated with a standard solution of caffeine as test substance. HPTLC of caffeine on silica gel with dichloromethane - methanol 9:1. Quantification with a video-documentation system.

J. Chromatogr. A 1155 (1), 112 - 123 (2007). Presentation of a novel strategy for the fractionation of complex peptide mixtures using two-dimensional planar electrochromatography/thin-layer chromatography. Migration of phosphopeptides is slower in the first dimension, based upon their anionic phosphate residues, and certain predominantly acidic phosphopeptides even migrate in the opposite direction, relative to the bulk of the peptides. Further distinguishability of phosphopeptides are based upon hydrophilicity in the second dimension, which permits a restricted region of the plate to be directly examined for the presence of phosphopeptides by mass spectrometry. Phosphopeptide analysis from the plates by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)-MS and tandem MS enabled peptide sequencing and identification.

Proceedings of Sino/ West German Symposium on Chromatography, Science Press,. Beijing, China 563-601 (1983). Using computer controlled TLC photometers, the whole domaine and flexibility of this analytical method can be used in a more accurate way.

Amer. Lab. 19, 23-25 (1987). Description of the advantages of constantly operating TLC over conventional TLC, column chromatography, or free flow analysis. Discussion of several contrasting views on the possibility of automating TLC, stressing on its significant increases in throughput. Some consideration on the chromatographic parameters, and instrumental parameters of constantly operating TLC.

J. Planar Chromatogr. 30, 411-417 (2017). HPTLC of paraben solutions and a complex matrix such as honey spiked with 5-hydroxymethylfurfural to study the influence of different application parameters on quantitative analysis. The investigated parameters included application geometries (spot, band and area) and their respective application modes (contact and spray-on), various dosage speeds, different band lengths, overspotted application versus application of mixture solutions as well as influence of the dosing volume on the result. These parameters have influence on method development and validation results.

Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 19-25. OPLC of ethinyl estradiole on silica gel with cyclohexane - ethyl acetate - chloroform 3:1:1. Detection by spraying with sulfuric acid and visual evaluation at 366 nm. The method is compared with the Official British and European Pharmacopoeia monographs. Only with the OPLC method it was possible to separate all impurities, i.e. 6-hydroxy derivatives (both isomers), the oxo-derivatives (6-oxo- and 16-oxo-), the 9(11)-dehydro and the 17-epi-derivatives from the estrone and estradiol. The HPLC method of the 3rd European Pharmacopoeia has to be changed slightly in order to analyze all impurities. The British Pharmacopoeia TLC method was not suitable because of its poor selectivity. Both OPLC and HPLC are suitable for purity testing. The OPLC method was preferred for process-validation because it shows a short analysis time and low eluent consumption (tenfold less than HPLC). The HPLC method was used for critical batches due to its better precision (3,5% RSD).