Chromatographia 78, 1409-1413 (2015). HPTLC of oligosaccharides (derived from native chondroitin sulfate and hyaluronan) on silica gel aluminum foils of either "standard" or MS grade with 1-butanol - formic acid - water 3:4:1. Detection by staining with orcinol/sulfuric acid reagent or by MALDI-TOF MS. The hRf values of the unsaturated disaccharides of chondroitin sulfate (C4S, one sulfate residue per disaccharide unit) and chondroitin (C0S, no sulfate) were 43 and 55, respectively. The thickness of the silica gel layer (200 or 100 µm) influenced the quality of the mass spectra: a thinner layer improved the achievable signal-to-noise ratio and minimized unwanted formylation of the GAG oligosaccharides.

Food Control. 79, 258-265 (2017). TLC-surface-enhanced Raman scattering of Sudan I in food on diatomite earth TLC plates fabricated by spin coating diatomite on glass slides with cyclohexane – ethyl acetate 6:1. Detection by depositing 2 mL concentrated gold nanoparticles three times. Quantification using a Raman microscope equipped with a CCD detector to acquire the surface-enhanced Raman scattering spectra. Excitation wavelength was 785 nm and the laser spot size was 2 mm in diameter. The method allowed for the determination of Sudan I in chili sauce down to 1 ppm (0.5 ng/zone) without sample preprocessing.

Food Chem. 243, 258-268 (2018). HPTLC of [6]-gingerol (1) and [6]-shogaol (2) in 17 ginger rhizomes and ginger-containing food products on silica gel with n-hexane – ethyl acetate 13:7. Detection by dipping into anisaldehyde sulfuric acid reagent (5 mL concentrated sulfuric acid was added to a mixture of 500 μL anisaldehyde, 10 mL acetic acid and 100 mL methanol), followed by heating at 110 °C for 5 min. Quantitative determination by absorbance measurement at 580 nm. The hRF values for (1) and (2) were 32 and 41, respectively. LOD and LOQ were 25 and 45 ng/zone for (1) and 20 and 40 ng/zone for (2), respectively. The primuline reagent (100 mg primuline in 200 mL acetone – water 4:1) was also investigated for detection, but it was not as sensitive. Polynomial calibrations ranged between 0.9982 and 0.9999. Their contents ranged 0.2–7.4 mg/g (1) and 0.2–3.0 mg/g (2) in the different products. Intermediate precisions were mostly ≤8 % for (1) and ≤10 % for (2) in the different food matrices. Effect-directed detection was performed via A. fischeri and B. subtilis bioassays, tyrosinase and AChE inhibition assays and DPPH* radical scavenging assay. Active unknown zones were further characterized by HPTLC-ESI-HRMS and assigned as [8]-gingerol and [10]-gingerol. Among others, further multi-detected zones were assigned to be [4]-gingerol, dehydro-[6]-gingerdione, dehydro-[6]-gingerol, dehydro-[8]-gingerol, dehydro-[10]-gingerol etc.

Lipids 17, 469-475 (1982). The total methyl esters of the fatty acids of tissue glycerides were converted into methoxy-acetoxy-mercury adducts. The adducts were fractionated into groups of equal numbers of ethylenic bands on silica with hexane - dioxane 6:4. Separation of the monoethylenic acids of equal chain length into the respective cis-and trans isomers by TLC on AgNO3-silica, acc. to known procedure.

Anal. Biochem. 154, 200-204 (1986). Two-dimensional TLC of lipid p and phospholipids on silica with chloroform - methanol - NH3 - water 65:35:3:2 for the first dimension and chloroform - acetone - methanol - acetic acid - water 50:20:10:10:5 for the second. Removal of the spots and treatment with ammonium molybdate. Measurement by spectrophotometry at 790 nm.

Study of the essential oil). J. Chinese Herb Med. 18, 386-388 (1987) (Zhong Caoyao). TLC of palmitic formate on silica with benzene. Detection by spraying with vanillin reagent. Elution with diethyl ether. Identification by IR, MS.

J. Chromatogr. 438, 329-337 (1988). Description of a method for acquiring identifiable FTIR spectra of well resolved compounds involving the combination of HPLC and TLC, each utilizing different separation modes. Measurement of IR spectra of TLC spots on silica by diffuse reflectance spectrometry. Detection limit, 8 ng.

Anal. Biochem. 175, 167-176 (1988). TLC of lipids from Gaucher’s disease tissue on silica with a) chloroform - methanol - water 65:25:4 and b) chloroform - methanol - water 55:45:10, containing 0.02% Coomassie brilliant blue R. Identification by direct MS analysis.