J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (5), 447-449 (2001). TLC on silica gel with benzene - acetic acid - formic acid 30:1:3. Detection under UV 365 nm, and by spraying with 2% FeCl3 in 50% ethanol and 2% potassium ferricyanide in 50% ethanol. Identification by finger print technique.

J. Chinese Trad. Patent Med. (Zhongchengyao) 25 (7), 583-584 (2004). TLC on silica gel with benzene - ethyl acetate - methanol - glacial acetic acid - water 16:10:8:4:3. Detection under daylight or UV light. Identification by finger print technique. TLC screening of many other animal's bile, such as duck bile, chicken bile, cow bile, sheep bile, pig bile and fish bile, etc. Discussion of the possibility of replacing snake bile with some other animal's bile containing similar components.

J. Planar Chromatogr. 21, 11-14 (2008). Pressurized planar electrochromatography (PPEC) of 4-cholesten-3-one, 4-androsten-17beta-ol-3-one, 17alpha-acetoxyprogesterone, androstenedione, 2’-acetonaphthone, benzanilide, 2-nitroaniline, hydrocortisone, and benzamide on spherical RP-18 phase, cut into 3.3 x 12 cm sections. The mobile phase was 55 % aqueous acetonitrile containing 5 mM acetate buffer pH 4.7. The apparatus for PPEC has been described by Nurok et. al., Anal. Chem. 78, 2823-2832 (2006). Nine analytes were separated in 2 min. Detection by direct analysis of the TLC plate using DESI coupled to a tandem mass spectrometer.

Anal. Chem. 77, 1207-1215 (2005). Desorption electrospray ionization (DESI) was demonstrated as a means to couple TLC with mass spectrometry. HPTLC of rhodamines on RP-2 and RP-8 with methanol - water 4:1 containing 200 mM ammonium acetate. HPTLC of FD&C dyes on RP-18 with water - acetone 7:3 containing 500 mM ammonium acetate. HPTLC of aspirin, acetaminophen and caffeine on silica gel with acetate - acetic acid 99:1. Tracks were scanned by moving the plate under computer control while directing the stationary DESI emitter charged droplet plume at the plate surface. Positioning of the DESI emitter, plate surface, and the atmospheric sampling orifice of the mass spectrometer were found to be crucial for obtaining maximum analyte signal levels. Desorption ionization from all TLC phases was not equivalent.

Rapid Commun. Mass Spectrom. 24, 1032-1038 (2010) HPTLC of glycosphingolipids (GSLs) on silica gel with chloroform - methanol - water 120:70:17. The plate was overlaid with GSL-specific bacteria, and the microbes were detected with primary antibodies and appropriate alkaline phosphatase labeled secondary antibodies, and by in situ MS analysis of bacteria-specific GSL receptors. The thin-layer chromatography infrared matrix-assisted laser desorption/ionization orthogonal time-of-flight mass spectrometry (TLC/IR-MALDI-o-TOF-MS) method represents one of the most powerful approaches for the detection of GSL receptors of microorganisms.

CBS 110, 2-4 (2013). HPTLC of caffeic acid, chlorogenic acid, and rutin hydrate on silica gel (prewashed with methanol and dried under vacuum at 50 °C for 30 min) with formic acid - ethyl acetate - water - methyl ethyl ketone 5:30:6:18 with chamber saturation for 5 min over a developing distance of 5 cm. Detection under UV 254 nm. Elution of substance zones with TLC-MS Interface using methanol and a flow-rate of 0.3 mL/min for 6 min. Evaporation of methanol under nitrogen, residue taken up with methanol-d4. Subsequent off-line quantitative 1H-NMR spectroscopic analysis of the residue, acquisition time 30 min. Linearity for all substances was confirmed in the range of 10 - 80 µg/mL. Recoveries were in the range of 100.5 % for chlorogenic acid and up to 103.4 % for caffeic acid, with precisions under 3.9 % (%RSD, n=3).

J. Planar Chromatogr. 28, 190-204 (2015). The analysis of multiple pesticide residues in environmental samples is often impossible in a single-step process. Comprehensive 2D planar chromatography on mono and bilayers, coupled-layer chromatography, combination of multidimensional planar chromatographic techniques, hyphenated methods are proved to be suitable.

J. of Chromatogr. A 1415, 155-160 (2015). Presentation of a technique for direct analysis of raw samples by TLC coupled with electrospray ionization mass spectrometry (ESI-MS) instead of conventional MS analysis, which for raw samples commonly requires time-consuming and laborious sample pretreatment and separation using HPLC or GC. The analytes of interest could be extracted, ionized and detected by ESI-MS with much reduced matrix interference because the interfering compounds were retained by the sorbent material of the TLC plate. Demonstration by applying in direct analysis of samples containing common interfering compounds, e.g. salts and detergents. Rapid detection and quantification of target analytes in raw samples showed that the TLC-ESI-MS method was simple, rapid, efficient and could be effectively applied in offline and online separation and detection of different components in raw samples, e.g. plant extracts.