J. Chinese Modern Med. & Pharm. 20 (31), 54-56 (2013). Paracetamol and chlorphenamine maleate granule are an anti cold medicine effective for cold induced nasal congestion, headache, sore throat, fever, etc. Artificial bezoar is one of the main ingredients and bilirubin is the key effective component in the medicine. For quality control, TLC of the extracts and bilirubin on silica gel with isooctane – ethyl acetate – glacial acetic acid 11:5:3, detection under white light. Quantitative determination of bilirubin by UV spectrophotometry. Demonstration by applying to real life samples from three pharmaceutical factories indicated that the method was suited for the quality control of the medicine.

Trends Anal. Chem. 82, 68-88 (2016). This review describes the application of ambient mass spectrometry for the analysis of pharmaceutical products and herbal medicines, including coupling TLC to DESI–MS for the analysis of analytes separated on TLC plates. The review also describes coupling TLC with direct analysis in real time (DART)-MS, which is easily achieved by positioning a TLC plate between the DART source and the MS inlet.

J. Chromatogr. Sci. 54 (7), 1096-1104 (2016). TLC of the extracts of the dried aerial parts of 12 plants of Cirsium species and five selected standards (naringin, apigenin, rutin, caffeic acid and chlorogenic acid) on silica gel with ethyl acetate – formic acid – acetic acid – water 24:3:3:8 on RP-18 phase with 5 % aqueous solution of formic acid – methanol 7:3 for the first development and the same system in the ratio of 1:1 for the second development. Analysis of the results by chemometrics. The comparison of individual Cirsium species and the identification of unknown species by using the similarity indices (Pearson's correlation coefficient, determination coefficient and congruence coefficient), distance indices (Euclidean distance, Manhattan distance and Chebyshev's distance) and Multi-Scale Structural SIMilarity. Based on chemometric analysis, the first extract of the widely grown species is identified as Cirsium arvense and the second one as Cirsium rivulare.

Phytochem. Anal. 30, 218-225 (2019). HPTLC of flavonoids in red wine, apple juice and rose flowers on silica gel with ethyl acetate – methanol – formic acid 90:10:1. Glycosylated flavonoids were identified by inorganic matrix‐assisted laser desorption ionisation time‐of‐flight mass spectrometry (MALDI‐TOF‐MS).

Detection under UV light. Elution with 0.5 N sulfuric acid. Photometry by absorbance at 285 nm.

J. Chromatogr. 365, 47-50 (1986). Proposal of a new method of identification and determination of nanogram amounts of substances: Separation by TLC followed by X-ray fluorescence analysis of the separated components on the chromatogram. Scanning by a collinated beam of primary X-radiation increases the definition of the chromatographic zone position.

Anal. Chim. Acta 199, 77-83 (1987). TLC of the product of the reaction between caprolactam and p-(N,N-dimethylamino)benzene-p’-azobenzoyl chloride on silica with benzene - ethyl acetate 12:3. Determination of caprolactam by adsorptive stripping voltammetry. Detection limit 0.2 µg/mL.

Analyst 113, 1605-1606 (1988). TLC of tyramine on silica with 1-butanol - acetic acid - water 15:3:2. Detection by spraying with Pauly’s reagent. Quantification by spectrophotometry at 237 nm after elution with 2M ammonia solution. The method is more reliable than HPLC method.