Chromatographia 46, 358-364 (1997). Report of the investigation and optimization of TLC/matrix-assisted laser desorption ionization (MALDI) coupling protocol, stressing on the fundamental coupling parameters which influence sensitivity and lateral analyte spreading, like extraction solvent selection, extraction time, and pressures. The upper analyte recovery limit, using extraction solvents with Rf values between 0.4 to 0.6 is estimated at 22%.

J. Planar Chromatogr. 11, 457-459 (1998). Description of a preconcentration method based on continuous elution and intense thermal desorption of the main volatile component in a narrow zone on the plate. A fiftyfold concentration of a model dye mixture from an aqueous solution was achieved on a TLC plate in 20 - 30 min. Silica gel, propan-2-ol as mobile phase and quantitation by densitometry at 530 nm. The technique enables determination of the amounts of common metals in drinking water.

J. Chinese Trad. & Herb. Drugs (Zhongcaoyao) 32 (5), 453-454 (2001). TLC on silica gel with 1) toluene - acetic acid - water 25:25:2. Detection by spraying with 10% sulfuric acid in ethanol and heating at 105°C for 5 min and under UV 365 nm. Identification by finger print technique.

J. Planar Chromatogr. 17, 449-453 (2004). In thin-layer radiochromatography the high sensitivity of the phosphor imaging analyzer can be further increased by multiple reading of the image plates, because the latent image is not lost quantitatively in the reading process. Summing of the chromatograms obtained in successive readings results in increased signal-to-noise ratio. Thus, by use of this approach either the exposure time can be shortened or higher sensitivity can be achieved. TLC of a14C-labeled test substance in rat serum on silica gel with chloroform - n-hexane - ethanol - ammonia 75:15:9:1. Detection by radioluminography.

Anal. Chem. 78, 5736-5743 (2006). Structural analysis of neutral glycosphingolipids by direct coupling of TLC to MALDI-QIT-TOF MS/MS. TLC of neutral glycosphingolipids on silica gel with chloroform – methanol – water 65:35:8. The underivatized glycosphingolipid spots were directly subjected to MALDI-QIT-TOF MS after addition of a matrix solution of 2,5-dihydrobenzoic acid in acetonitrile – water 1:1, which proved to be suitable for MS/MS analysis with high sensitivity. MS/MS and MS/MS/MS spectra reveal the ceramide and long-chain base structures, as well as the sugar sequences. Furthermore, derivatization with primuline (0,01 % in acetone – water, 4:1), a non-destructive fluorochrome for lipid detection, was used to locate glycosphingolipids on a TLC plate prior the MS analysis. The coupling of TLC-immunostaining of glycosphingolipids to MALDI-QIT-TOF MS/MS is shown to be a powerful method to identify both the antibody-specific sugar and the ceramide structures.

Anal. Chem., 58, 2708-2714 (1986). Description of sample transfer approach to TLC/FTIR in which several separated components are moved simultaneously from the TLC plate to an IR transparent substrate (Ge-Sb-Se glass) prior to measurement of its diffuse reflectance spectrum. Transfer with minimal sample loss, decomposition, or contamination compared with previous techniques. Sample transfer, TLC/FTIR

Merck KGaA (Ed.): Chromatographie - Chronologie einer Analysentechnik - Praxis, Status, Trends, GIT Verlag mbH, Darmstadt, 96-100, ISBN 3-928865-21-8 (1996). Correlation between instrumental thin-layer chromatography and GLP (Good Laboratory Practice). Each step in TLC, e.g. sample application, development, evaluation, pre- or post chromatographic derivatization, is fully automated. The advantages and possibilities of each automated step are discussed. Instrumental TLC shows good reproducibility and high flexibility. Documentation regarding GLP is demonstrated by reports. Automated validation of instruments is shown.

Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 57-65. Combined application of OPLC-DAR (overpressured layer chromatography - digital autoradiography) and HPLC-RD (high performance liquid chromatography - radioactive detector) solves complex problems appearing during in vivo metabolism study. OPLC-DAR is more sensitive than HPLC-RD and excellent for getting fast information about the existence of conjugates. In case of small amount of sample with low activity, OPLC-DAR is the only possibility for getting qualitative and quantitative information.