J. Chin. Trad. Med. (Zhongguo Zhongyao Zazhi) 20, 349-350 (1995). Two-dimensional TLC on silica with chloroform - methanol -water 65:35:1 and chloroform - methanol - acetone 2:1:2. Detection by exposure to iodine vapor. Quantification by colorimetry after elution.

Chinese Anal. Chem. (Fenxi Huaxue) 24, 1134-1138 (1996). TLC on silica with methanol - ethyl acetate 7:3. Detection by exposure to iodine vapor or by spraying with 0.01 mol/L PdCl2. Quantification by coulometric titration.

Indian Drugs 34 (9), 512-519 (1997). HPTLC on silica gel with methanol - water - NH3 18:2:1. Ofloxacin was used as internal standard. Densitometry at 280 nm.

J. Planar Chromatogr. 13, 76-81 (2000). Report of the generation and characterization of an impurity profile of a mixture of a pharmaceutical compound (UK 224671) and known related impurities which were separated by TLC. The plate was prepared for TLC-MALDI-TOFMS by electrospraying the surface with a solution of a-cyano-4-hydroxycinnamic acid by means of an electrospray deposition device. TLC on silica gel with dichloromethane - methanol - NH3 (35%) 60:10:1. Visualization by UV 254 nm.

Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 3-13. OPLC (HPTLC/TLC) of trans-resveratrol, trans-trans-farnesol, and capsaicin on silica gel with e.g. chloroform - methanol - hexane 20:5:2 or chloroform - acetonitrile 2:3 in linear, one- and two-directional and also two-dimensional off-line separation technique. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide was used for the visualization of antimicrobial activity. Bioautographic detection using an inoculation process with Bacillus subtilis resp. Botrytis cinerea and Uromyces phaseoli.

J. Planar Chromatogr. 18, 44-50 (2005). A new hyphenated technique that enables coupling of TLC with time-of-flight secondary-ion mass spectrometry (TOF-SIMS) has been used for identification of gibberellic acid as model analyte. When TLC and TOF-SIMS are ccoupled on-line the chromatographic thin-layer must be modified to avoid TOF-SIMS background signal activity from the chromatographic material or solvents used. Two different types of TLC plates - alumium backed silica gel and monolithic silica gel - were used. TOF-SIMS enables analyte detection with high mass resolution at a level of concentration not achieved by other methods.

J. of Chromatogr. B 879 (30), 3565-4573 (2011). Presentation of a novel “target constituent knock-out” strategy applied for preliminary screening of antibacterial constituents in Calculus bovis. The strategy contained the following steps: 1) the single constituents (A-F) in C. bovis samples were knocked out by TLC on silica gel with toluene - acetic acid - water 30:25:2, detection under UV 366 nm; 2) the knocked-out constituents were identified by UPLC–ELSD; 3) the antibacterial activities of the knocked-out constituents and C. bovis samples on Staphylococcus aureus were evaluated by microcalorimetry combined with principal component analysis; 4) the activities of the knocked-out constituents and the total extract of C. bovis, also the interaction properties between these single constituents and the total extract were elucidated. The strategy proved to be useful for screening active constituents and elucidating the multi-component interactions in C. bovis, and helpful in understanding the pharmacodynamic actions and the quality control of traditional Chinese medicines.

Anal. Chim. Acta 218, 217-229 (1989). Illustration of the use of phase-transition matrix in the direct MS analysis of TLC plates to increase both the absolute signal intensity and the duration of the analyte signal in comparison with analyses without solvent or with liquid matrixes such as glycerol. Preservation of the original resolution of TLC separation by extraction of sample into the matrix without diffusion. Use of spatially resolved images of individual ions in pattern recognition programs that establish the correlations between all of the ions in the mass spectrum of a particular sample spot.