Food Chem. 210, 200-211 (2016). Review of the principles and applications of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) in food science and related fields. The review described the combination of MALDI-MSI and HPTLC, including the visualization of phospholipids in chicken egg yolk on silica gel with acetonitrile – water 1:1.

J. Chromatogr. A 1460, 181-189 (2016). Development of an online simultaneous TLC-MS analysis system, liquid thin layer chromatography–mass spectrometry (LTLC-MS), using regular TLC plates combined with a newly developed ambient ionization method, glow discharge-matrix assisted infrared desorption ionization (GD-MAIRDI), and forced-flow TLC (FFTLC) technique. This guarantees that the MS detection process does not disturb the TLC separation process throughout the analysis, but successfully synchronizes the TLC separation and MS detection processes like GC–MS and HPLC–MS do. Demonstration by applying to the analysis of the two main components of a pain relief pills proved that the technique opens up new possibilities of combining TLC with other techniques like MS, etc.

J. Chromatogr. A 1560, 97-103 (2018). Proposal of a simple and convenient on-spot derivatization approach for the modification of hydroxyl-containing compounds for their analysis by TLC/MALDI. Post-chromatographic acylation of separated analytes with 3-bromopropionyl chloride with simultaneous quaternization of pyridine, resulted in derivatives with permanent positive charges, which, in contrast to the initial alcohols not ionizable under TLC/MALDI conditions, reveal intense peaks of their cationic moieties in MALDI mass spectra recorded directly from TLC plates. Demonstration of the method by applying to a series of mammalian and plant sterols, phenols and terpene alcohols.

Anal. Chem. 56, 2935-2939 (1984). Presentation of a method for obtaining IR information from mg level of material in situ on TLC plates using diffuse reflectance FTIR spectrometry with reference laser aiding in spot alignment. Subtraction of plate contributions to background from the resultant IR spectra by the FTIR data system. Test of a variety of different materials on various plate types. The technique allows for both spot identification and functional group information useful in the subsequent characterization of unknown impurity.

Quantification after elution by fluorescence spectrophotometry at 360 nm excitation and 510 nm emission.

Anal. Biochem. 163, 27-35 (1987). TLC of various glycosphingolipids on silica coated with a thin film of poly (iso-Bu methacrylate). Plate placed in a chamber containing metabolically radiolabelled cells, sealed and centrifuged to bring the cells into contact with the surface of the plate. Development of the cell adhesion blocked by a solution of N-acetyl-glycosamine in various solvents. Detection by autoradiography. Detection limit, 8 pmol.

J. Chromatogr. 431, 431-437 (1988). TLC of chlorinated phenols on silica with a) benzene - methanol 95:5, b) petrol ether - benzene 1:1, c) hexane - acetone 30:10, d) benzene - chloroform 70:30 and e) hexane - benzene - ethyl acetate 6:4:1. Detection by spraying with ammoniacal acetone solution of silver nitrate and exposure to UV light for 5 min. Combination of TLC and GC methods.

Anal. Biochem. 175, 258-262 (1988). TLC of tetrodotoxin and related substances, anhydro tetrodotoxin and tetrodonic acid on silica with pyridine - ethyl acetate - acetic acid - water 15:5:3:4. Identification by fast atom bombardement MS. Detection limit, 0.1 µg.