Rapid Commun. Mass Spectrom. 24, 659-666 (2010). HPTLC of rhodamine B and five de-ethylated transformation products (N,N,N‘-tryethylrhodamine (1), N,N‘-dyethylrhodamine (2), N,N-dyethylrhodamine (3), N-ethylrhodamine (4), and rhodamine (5)) in groundwater on silica gel by automated multiple development with a 23-step gradient based on methanol (with the addition of formic acid) and dichloromethane. The drying time after each step was 2 min. For detection by bioluminescence the plate was dipped into a suspension of Vibrio fischeri for 2 s at a speed of 3 cm/s. The hRf were 72, 66, 60, 53, 48, and 36 for compounds (1) - (5). Combination of different separation and detection techniques enabled a fast and effective screening of the groundwater sample.

J. Planar Chromatogr. 26, 172-179 (2013). OPLC with on-line detection and fractionation, in-situ sample clean-up in the planar layer adsorbent bed, direct bioautography (DB), OPLC–MS, solid phase microextraction (SPME)–GC–MS, and LC–MS/MS for the bioassay-guided isolation and characterization of bioactive compounds from chamomile flower extract. The bioassay-guided isolation of antibacterial chamomile components was based on OPLC separation with on-line detection and fractionation combined with previous sample clean-up in-situ in the adsorbent bed after sample application. First the adsorbent layer was partially pre-weted between the edge of the layer and the sample application zone with the goal to fill up the “dead” area behind the trough, which leads the components to leave the adsorbent layer during the clean-up step. With this process, the zone behind the trough can be protected from stucking of any components in it, otherwise the stucked compounds could be detected continuously during the separation/detection/fraction collection. During the in-situ sample clean-up the mobile phase flow was in the opposite direction, from outlet toward inlet of the chamber. In this step the load of the adsorbent can be decreased for the fractionation, which is done in the normal direction of the mobile phase.

Giovanni Olini (1200 AD) and St. Nicolosa Bursa (1500 AD). J. Planar Chromatogr. 28, 205-212 (2015). TLC of (1) proteins, (2) resins, (3) sugars, and (4) waxes from fragments of the surface-coating material found on mummified bodies on silica gel for (2), (3), and (4), and on cellulose phase for (1) with n-butanol - acetic acid - water 4:1:1 for (1), benzene - methanol 19:1 for (2), acetonitrile - water 17:3 for (3), and petroleum ether - diethyl ether - acetic acid 90:10:1 for (4). Detection was performed after derivatization with ninhydrine reagent for (1), and iodine reagent for (2), (3) and (4). The combination of TLC and other chemical methods proved to be an effective and low-cost tool for obtaining valuable information during the archaeological investigation.

Planta Medica 82 (15), 1351-1358 (2016). The barks of forest trees (Acer pseudoplatanus, Alnus glutinosa, Fagus sylvatica, Fraxinus excelsior, Larix decidua, Picea abies, Populus robusta, Populus tremula, Prunus avium, Quercus robur) were successively extracted with n-heptane, methanol, and methanol/water; extracts and gentamycin were applied on TLC plates (but not developed; no TLC), which were sterilised, covered with a Mueller-Hinton agar medium containing a Staphylococcus aureus CIP 53.154 suspension, incubated at 37°C for 24 h, and revealed with MTT. All the methanolic extracts were active, as well as some other, the most active being those of Q. robur, L. decidua, and P. abies.

J. Chromatogr. Sci. 54 (9), 1661-1669 (2016). Development of two sensitive and accurate stability-indicating chromatographic methods for the determination of rafoxanide (RFX): 1) by TLC on silica gel with chloroform – ethyl acetate – toluene – ammonia 50:40:3:1, determination by densitometry at 280 nm; 2) by UPLC. Identification of the degradation products by mass spectrometry and IR spectroscopy. Both proposed methods proved to be accurate, robust, specific and suitable for application as stability-indicating methods for routine analysis of RFX in quality control laboratories.

J. AOAC Int. 101, 1993-2000 (2018). HPTLC of neutral lipids in fatty acid methyl ester (FAME)-derived biodiesel (1) and sphingolipids in human plasma (2) on LiChrospher or silica gel with a 4-step gradient based on t-butyl methyl ether, dichloromethane, and n-heptane for (1) and a 7-step gradient based on methanol and dichloromethane for (2). Detection of neutral lipids by dipping into a methanolic primuline solution (0.02 g/100 mL), followed by fluorescence recording at 366/>400 nm. Detection of sphingolipids by absorbance measurement at 190 nm. Zones of interest were eluted into an electrospray ionization-ion trap MS (ESI-MS) using a TLC-MS interface. _x000D_

Japanese Fisheries (Nippon Suisan Gakkaishi) 51, 1203 (1985). TLC on cellulose with five solvent systems: a) ethyl acetate - acetic acid - water 3:2:1, b) chloroform - methanol -28 % NH3 3:2:1, c) butanol - acetone - 85 % formate -water 10:10:2:5, d) butanol - acetone - 28 % NH3 - water 10:10:2:5, e) butanol - acetone - water 4:2:1. Identification by Rf comparison, confirmation by IR.

J. Chromatogr. 369, 435-439 (1986). TLC examination of phorbol on silica with 10 different solvent systems, and on long-chain-hydrocarbon-bonded silica with 4 different combinations of methanol - acetonitrile -water. Detection under UV at 254 nm and by spraying with vanillin - sulfuric acid -ethanol 3:0.5:100 and heating at120 °C.