J. of Chromatogr. A 1218 (19), 2684-2691 (2011). Review on TLC/bioautography. Discussion of three versions of bioautography, i.e. contact, immersion and direct bioautography. The focus is put on direct bioautography and many applications are quoted, not only for testing various groups of compounds, but also for investigating biochemical processes and factors influencing bacterial growth. Various related methods can be included into direct bioautography, of which TLC-bioluminescence screening is the most promising one.

R.E.Kaiser (Ed) "Proc. of the 3rd mt. Symp. on Instr. HPTLC, Wuerzburg", Bad Duerkheim 1985, 281-311. Strategy of pre-and postchromatographic derivatization reactions with different chromophores or fluorophores. Examples of derivatization procedures for quantitative determination: diethylstilbestrol, ethinylestradiol, gentamycins, antiepileptics, selenium in human serum. Description of the optimization of sample preparation steps. Statistical model for the evaluation of systematic errors.

Chinese J. Chem. 4, 35-36 (1987) (Huaxue Tongbao). Use of N,N-dimethyl-a-naphthylamine-5-sulfonyl chloride as a pre-chromatography derivatizing agent for some pesticides and herbicides. Spraying with tris(2-hydroxyethyl)-amine or paraffin oil after development. Detection by fluorodensitometry at 320 nm excitation and 500 nm emission.

J. Chromatogr. 504, 456-463 (1990). TLC of title compounds on cellulose or silica with one of the following solvents; 1) 2-methylpropan-2-ol - butanone - propanone - methanol - water - NH3 40:20:20:1:14:5, 2) butanol - propanone - acetic acid - water 35:35:10:20. Detection: spraying with 2,4-dinitrophenylpyridium chloride in methanol 200 mg/L for cellulose and 100 mg/L for silica - placing the plate in sealed glass tank with alkaline atmosphere for 15 min, keeping plate in the dark for 24 h before recording the fluorescence.

Analyst 119, 415-516 (1994). Presentation of a spray reagent for the detection of carbamate insecticide carbaryl, consisting of 1% ammonium cerium (IV) nitrate in 20%(v/v) hydrochloric acid, which reacts exclusively with the hydrolysis product of carbaryl, 1-naphthol, and forms a violet complex. Sensitivity, about 100 ng.

J. Chromatogr. A 750, 403-407 (1996). TLC of organophosphorus insecticides, carbamates and pentachlorophenol, etc. on silica with tetrahydrofuran - hexane 7:25, and hexane - ethyl acetate 3:2. Detection by using biological or biochemical tests. Investigation of the relationship between the signal of the inhibition of cholinesterase and the concentration of the inhibitor using a constant enzyme concentration and a constant incubation time. Quantification by densitometry or videodensitometry.

Anal. Chim. Acta 488, 203-209 (2003). Dansylation of aromatic carboxyl compounds (i.e. aspirin), aromatic primary amines, and aliphatic carboxyl compounds in 1 M Na2CO3 buffer at pH 11. TLC on silica gel of fluorescent labeled analytes using methylene chloride, ethyl acetate, acetone, or desired mixture of the solvents. Methylene chloride was superior to ethyl acetate or acetone. Fluorescent analytes were observed under UV lamp. Limits of detection for dansylated carboxyl compounds was 1-5 µg.

Acta Chromatographica 22 (2), 227-236 (2010), DOI:10.1556/AChrom.22.2010.2.6. TLC of piperine, the bioactive constituent of black pepper (Piper nigrum), on silica gel with dichloromethane – ethyl acetate 9:1 at 30 °C in a twin-trough chamber saturated for 30 min. Detection under UV light at 254 nm and documentation with a digital camera. Based on the image a density profile plot was established by Scion Image software, which allowed to calculate the concentration of piperine by comparison of the peak areas of samples and piperine standards. The linearity was in the range of 24-84 ng/zone (r2=0.9927). The limits of detection and quantitation were 0.35 and 1.05 ng/zone, respectively. Precision (repeatability, n=6) and intermediate precision (2 days, n=12) both are below 2.6 %RSD. Recovery is between 96.7-101.4 %.