1. Effect of temperature on Rf values.) (Japanese). Kagaku to Kyoiku (Jap. J. Educ.) 42, 206-210 (1994). Examination of the effects of temperature on Rf values in TLC, with some dyes and simple organic compounds, e.g. nitrobenzene, phenol, etc., and steroids as analytes.

J. Chem. Educ. 73, 77 (1996). Description of a novel TLC developing chamber that greatly enhanced the efficiency of screening column chromatographic fractions in laboratory.

J. Liqu. Chromatogr. 23, 531-549 (2000). Description of the basic features of planar displacement chromatography, including differences from elution chromatography, and that of column displacement chromatography, the possibility of using spacer displacement chromatography. Parameters limiting displacement chromatography, and their optimization to achieve better separation are discussed. Displacement thin-layer chromatography has the advantage of planar arrangement of the stationary phase, and opens new possibilities for analytical separations.

J. Planar Chromatogr. 16, 176-182 (2003). A commercially available horizontal DS chamber has been applied to the planar electrochromatographic separation of a dye mixture and of the enantiomers of phenylalanine on non-polar stationary phases with ethanol-buffer or acetonitrile-buffer as mobile phases. The mobile phases were driven electroosmotically by applying a 50-250 V/cm field along a 10 cm RP-18 plate. Compared with conventional TLC separations the efficiency of electrochromatography was higher and the development time shorter. Conventional HPTLC of a dye mixture (1-aminoanthraquinone, fat brown, 4-diethylaminoazobenzene, 4-hydroxybenzeneazonaphthol-2, 4-(4-(N,N-ethylethanol)benzeneazo)-N-methylphthalimide, 4-nitroaniline) on RP-18 with 80% ethanol in pH 10 buffer (diluted 1:3 with water) and of DL-phenylalanine on CHIR plates in a horizontal chamber after presaturation; detection of DL-phenylalanine by application of ninhydrin solution before densitometry. Densitometry at 440 nm for the dye test mixture and at 520 nm for DL-phenylalanine.

J. Liq. Chrom. Rel. Technol. 27, 1105-1113 (2004). Two-dimensional (2D) TLC has been mostly used for the separation of a large number of compounds that cannot be separated in a single dimension TLC experiment. In 2D TLC, separation is on one surface spread along the entire area of the plate. The resolving power of the 2D TLC has great application, especially in the areas of biochemistry, biology, natural products, pharmaceuticals, and environmental analysis. Detailed description of two-dimensional development, two-dimensional development on bilayers, two-dimensional separation by TLC/electrophoresis, and chromatogram evaluation.

Ind. J. Pharma. Sci. 72(5), 629-632 (2010). TLC of almotriptan malate on silica gel aluminum foil with butanol - acetic acid - water 3:1:1. Quantitative determination by densitometry in absorbance mode at 300 nm. The calibration curve was linear over the range of 100-700 ng/band for almotriptan malate. The method was successfully applied to the analysis of drug in a pharmaceutical form. 

Anal. Biochem. 149, 480-483 (1985). Description of a simple visualization method for sulfolipids on TLC plate, based upon formation of complex between anionic sulfolipid and blue cationic compounds by spraying with an acidic solution of azure A. Detection limit 0.5 nmole of sulfolipids without interference from other lipids. Visualization of sulfolipids as clear dark blue bands on a light blue background after the unbound dye washed out by brief soaking in methanol.

Ein neues Verfahren zum Nachweis von Substanzen in der Dünnschichtchromatographie. I. Nachweis von Flavoniden. Microwave vaporizing-technique of substances on thin-layer chromatogramms. I. Detection of flavonoids. (German). Deutsche Apotheker Zeitung 129, 2178-2179 (1989). New method for the detection of substances on thin-layer chromatogramms. The procedure consists in the vaporizing of the reagent using a microwave-oven. The advantage of the method is illustrated by the flavonoid detection TLC of flavonoids on silica with ethyl acetate - formic acid - water 8:1:1.