J. Chromatogr. A 1530, 197-203 (2017). Evaluation of the phenolic and stigmasterol content and comparison of the antioxidant and antidiabetic activities by HPTLC combined with DPPH* free radical method and α-amylase assay towards ethanol and ethyl acetate extracts of 10 marine macroalgae species (3 chlorophyta, 4 phaeophyta and 3 rhodophyta). HPTLC on silica gel with n-hexane – ethyl acetate – acetic acid 20:9:1 over 80 mm. Detection either by dipping into 0.4 % DPPH* solution followed by storing in the dark for 30 min before evaluation, or by dipping into anisaldehyde – sulfuric acid reagent or neutralized ferric chloride solution (prepared by adding dilute sodium hydroxide to freshly prepared 2 % ferric chloride in methanol until precipitation occurs, followed by filtering), both followed by heating at 100 °C for 10 min. Quantitative evaluation by absorbance measurement at 470 nm for detection of α-amylase inhibition and at 550 nm for stigmasterol. The results showed higher antioxidant activity in the ethyl acetate extracts than in ethanol extracts, and higher amounts of fucoxanthin, stigmasterol and α-amylase inhibitory activities in ethyl acetate extracts. The results proved the relation of higher α-amylase inhibition in ethyl acetate extracts due to the presence of triterpenes and sterols, and no contribution of fucoxanthin to it.

Virgaureasaponin 1, a new bisdesmosidic triterpenoid saponin of Solidago virgaurea L. Pharmazie 42, 541-543 (1987). TLC of saponins on silica with chloroform - methanol - water 15:10:2, with chloroform - methanol 9:1 and with n-butanol - ethanol - water 4:1:5 (upper phase). Detection reagents: blood gelatin for saponins, anisaldehyde - sulfuric acid for saponins and sapogenins and thymol - sulfuric acid for sugars. A new triterpenesaponin isolated and identified.

J. of Natural Products 51, 504-508 (1988). TLC of cucurbitacin B on silica and silica PF254 with ethyl acetate - methanol - water 10:1:3 (upper layer) and chloroform - methanol 11:0.4. Detection with VAP reagent (2% ethanol solution of vanillin, 85% o-phosphoric acid and ethanol 1:2:8) and heating for 10 min. at 120°C, examination under UV 365 nm.

J. of Natural Products 53, 196-198 (1989). TLC on silica with diethyl ether - hexane 4:3. Visualization by UV or by spraying with anisaldehyde reagent.

Phytochemistry 30, 1957-1962 (1991). TLC of clerodane diterpenes on silica with chloroform - methanol 9:1, ethyl acetate - methanol 9:1 and petrol ether - acetone 9:1. Detection with anisaldehyde reagent. (Excellent resolution of clerodane-type diterpenes. Also TLC of berberine and related alkaloids on silica with methanol - water mixtures, as well as column chromatography.

J. Chromatogr. A 715, 372-375 (1995). TLC of 32 steroids and triterpenes on silver nitrate-impregnated silica with 1) hexane - ethyl acetate 3:1, 2:1, 2) hexane - ether 10:1, 5:1, 3) hexane, 4) hexane - toluene 10:1. Report of the Rf values.

Chinese J. Chromatogr. (Sepu) 15, 328-330 (1997). TLC of ipomeamarone, ipomeamaronol, ipomeanine and 4-ipomeanol on silica with 1) hexane - ethyl acetate 4:1, 2) petrol ether - ethyl acetate 2:1, 3) benzene - methanol 9:1. Detection by spraying with Ehrlich's reagent. Quantification by densitometry at 527 nm. Detection limit 5 ng - 20 ng.

J. Agric. Food Chem. 49, 3187-3197 (2001). Preparative TLC of fatty acid esters of triterpenols and sterols, triterpene diols, free triterpenols, free sterols, fatty acid esters of triterpene triols on silica gel with n-hexane - ethyl acetate 6:1.