Chinese J. Herb Med. (Zhongcaoyao) 25, 295 (1994). TLC of patchouli alcohol on silica with petrol ether (30 - 60°C) - ethyl acetate - acetic acid 90:10:0.2. Detection by exposure to iodine vapor. Quantification by densitometry at 590 nm.

Phytochemistry 47, 231-235 (1998). Isolation of a new sesquiterpene trimer, consisting of three lindenane units, by preparative TLC on silica with ether - methanol 25:1. Elution of a yellow fluorescent band and further purification by preparative TLC on silica with chloroform - methanol 13:1. Isolation of additional sesquiterpenes by successive preparative TLC on silica with chloroform - methanol 50:1 and hexane - ethyl acetate 2:1.

JAOAC Int. 84, 1232-1241 (2001). HPTLC of ginkgolide A, ginkgolide B, ginkgolide C, and bilobalide on silica gel, modified by dipping into a 4% sodium acetate - 60% ethanol solution for 2 s and activated at 90°C for 10 min, with toluene - ethyl acetate - acetone - methanol 50:25:25:3 after equilibration of the plate for 30 min in a conditioning tray containing sulfuric acid aqueous solution (about 47% relative humidity). Detection after heating of the plate for 30 min at 160°C and observation of the fluorescent chromatogram under UV 366 nm. Quantification by densitometry at 366 nm.

Planta med. 68, 55-59 (2002). Analytical and preparative TLC of 3ß-angeloyloxy-8a-hydroxy-6ß-methoxyeremophil-7(11),9(10)-dien-8,12-olide, 2:3ß-angeloyloxy-6ß,8a-dihydroxyeremophil-7(11),9(10)dien-8,12-olide, 5:3,8-oxo-eremophila-6,9-dien-12-oic acid, 3:3ß-angeloyloxy-8a-hydroxy-6ß-ethoxyeremophil-7(11),9(10)-dien-8,12-olide, 4:3ß-angeloyloxy-8-oxo-eremophila-6,9-dien-12-oic acid on silica gel with chloroform - methanol 12:3:2 and dichloromethane - methanol 10:1.

J. AOAC Int. 89, 1-7 (2006). TLC of masticadienonic and 3-hydroxymasticadienonic acid on silica gel with hexane - acetone - formic acid - acetic acid 30:10:1:1. Quantitation by determination of the absorption at 200 nm. Detection by dipping into anisaldehyde - sulfuric acid reagent for 1 sec and heating at 100 °C for 5 min. Limit of detection was 0.1-0.2 µg/mL.

J. Planar. Chromatogr. 28, 173-177 (2015). HPTLC of (1) sclareol, (2) linalool, (3) linalyl acetate, and (4) carvone on silica gel with n-hexane - ethyl acetate 17:3. Detection at UV 254 nm and in daylight after dipping in vanillin - sulfuric acid followed by heating at 110 °C for 5 min. The presence of (1) in Clary Sage essential oil was demonstrated by 2D on silica gel with toluene - ethanol 17:3 in the first direction and n-hexane - acetone in the second direction, and on RP-18 phases with acetonitrile - water 7:3. Bioassays were performed by the direct bioautographic system using a Gram-negative test bacteria (Xanthomanas euvesicatoria), the luminescence gene-tagged Arabidopsis pathogen (Pseudomonas syringae pv. maculicola) and the luminescent marine bacterium (Aliivibrio fischeri) as well as a Gram-positive soil bacterium (Bacillus subtilis). For Xanthomanas euvesicatoria and Bacillus subtilis, the dried layers were dipped into cell suspension culture and after 2 h incubation in a vapor chamber at 28 °C, they were immersed into an aqueous MTT solution. The bright zones against the darker background indicate the presence of antibacterial components. For luminescent bacterial strains, the plate was dipped into bacteria suspension and evaluated with a computer-controlled cooled low-light camera. Images of bioautograms were directly recorded with an exposition time of 5 min for Aliivibrio fischeri and 15 min for Pseudomonas syringae pv. maculicola. The dark spots lacking luminescence indicate the antibacterial activity. Sclareol inhibited all tested bacteria, linalool and carvone showed antibacterial effect against all Gram-negative strains tested, while linalyl acetate only against Xanthomanas euvesicatoria and Aliivibrio fischeri.

Chinese J.Pharm.Anal. 8, 33-36 (1988) (Yaowu Fenxi Zazhi). TLC of d-menthone and 1-pulegone on alkali-modified silica with petrol ether - ethyl acetate 9:1. Detection by spraying with vanillin - sulfuric acid - ethanol 1:1:18 and heating at 80 °C for 20 min.

Olaj, Szappan, Kozmetika 38, 28-32 (1989). TLC of perfume oils on silica with benzene - ethyl acetate 8:2, dichloromethane - petrol ether 7:3 and toluene - ethyl acetate 98:2. Visualization under UV and by spraying with 3% sulfuric acid - vanillin reagent followed by heating at 105°C for 5 min.