Planta med. 65, 754-756 (1999). Preparative TLC of rubiadin-1-methyl ether (1-methoxy-3-hydroxy-2methylanthrquinone) on silica gel with toluene - ethyl acetate (9:1).

J. Planar Chromatogr. 22, 49-53 (2009). TLC of geranylgeraniol and plaunotol on silica gel with chloroform - n-propanol 24:1 or 48:1, or with ethyl acetate, in saturated chambers. Quantitative determination by absorbance measurement at 210 nm. Detection of plaunotol by exposure to iodine vapor for 10 min. The acyclic diterpenoid plaunotol present in Croton stellatopilosus leaves is a hydroxylation product, catalyzed by the enzyme geranylgeraniol-18-hydroxylase. The activity of the enzyme in cell-free extracts of C. stellatopilosus leaves was previously reported. In this study a new mobile phase (ethyl acetate) was used for determination of geranylgeraniol-18-hydroxylase in the 20,000 g and 100,000 g precipitates of the crude extracts. In addition ethyl acetate successfully separated plaunotol from various cytochrome P-450 inhibitors (ancymidol, metyrapone, and miconazole) frequently used for biochemical characterization of the hydroxylase enzymes.

J. Planar Chromatogr. 26, 260-266 (2013). HPTLC of of iridoids 10-O-trans-p-coumaroylcatalpol (1), 4-hydroxy-E-globularinin (2) and premnosidic acid (3) in the stem bark of Premna integrifolia on silica gel with ethyl acetate - methanol - water - acetic acid 40:6:3:1. Detection by dipping into vanillin - sulphuric acid reagent (1 % vanillin in ethanol - sulfuric acid 19:1), followed by heating at 110 ºC for 3 min. Quantification by absorbance measurement at 510 nm. The hRf values for (1) to (3) were 52, 41 and 33, respectively. Linearity was in the range of 1-10 µg/zone for (1) to (3). LOD and LOQ were 198 and 663 ng/zone for (1), 312 and 1040 ng/zone for (2) and 200 and 666 ng/zone for (3), respectively. Average recoveries for (1) to (3) were found to be 97.3 %, 98.3 % and 97.6 %, respectively. Intermediate/interday/intra-day precision was below 2 % (n=9).

Phytochemistry 24, 1587-1591 (1985). TLC of cucurbitacins on silica with chloroform - methanol 9:1. Detection with vanillin - phosphoric acid reagent. For the detection of alpha-ketolcucurbitacins, the plates were sprayed with a 1:1 mixture of triphenyltetrazolium chloride (4 % in ethanol) and NaOH 1 N. Flavonoid glycosides were separated on silica with ethyl acetate - formic acid - acetic acid - water 100:11:11:27. Detection with diphenylboryloxyethylamine reagent, followed by PEG 4000 (5 % in ethanol, UV 365 nm.

Acta Pharm. Hungarica 57, 228-238 (1987). TLC and OPTLC of artemorin, partenolide, tatridin, costunolide, cnicin, santamarin, umbellifolide, taurin, reynosin (sesquiterpene lactone compounds). TLC on silica with benzene - acetonitrile - n-hexane 30:21:20 or carbon tetrachloride - acetonitrile 40:15. Detection by spraying with 1. 2% sulfuric acid in ethanol, then heating at 110 °C for 2-3 min.; 2. phosphomolybdic acid reagent, then heating at 100 °C for 2 min. Densitometry by absorbance at 520 and 600 nm. OPTLC on silica with carbon tetrachloride - acetonitrile 4:1.5, n-hexane - ethyl acetate 2:1 or 7:3, benzene - acetonitrile - n-hexane 3:2.1:2. Detection and evaluation methods same as in TLC.

J. of Natural Products 51, 1249-1250 (1988). TLC of nagilactone F, G, E, and E acetate on silica with isopropylether - hexane - ethyl acetate 4:2:1. Identification by comparison with authentic samples.

Phytochemistry 28, 2971-2979 (1989). TLC of monoterpene glucosides on silica with chloroform - methanol 100:3 and 95:1, benzene - acetone 7:3 or 1:1. Visualization under UV or by exposing to iodine vapor.

Planta Med. 58, 228 (1992). TLC of a dichloromethane extract of the stem bark of Fagaropsis glabra with dichloromethane – methanol 98:2. Detection by spraying with Ehrlich’s reagent followed by immersion in HCL vapors. Prep. separation by centrifugal TLC on silica with dichloromethane – methanol 98:2 to 95:5.