J. Pharm. Biomed. Anal. 189, 113488 (2020). Various extracts from red alga Plocamium dilatatum (Plocamiaceae), green alga Codium fragile tasmanicum (Codiaceae) and brown algae Carpoglossum confluens (1), Cystophora platylobium (2) and C. retorta (3) (Sargassaceae), Ecklonia radiata (Lessoniaceae), Hormosira banksia (Hormosiraceae), Phyllospora comosa (4) (Seirococcaceae) were separated on HPTLC silica gel with n-hexane – ethyl acetate – acetic acid 70:27:3. Detection A) for antioxidant activity by spraying with methanolic DPPH solution, followed by waiting for 30 min at room temperature; B) for steroids and terpenes with anisaldehyde - sulfuric acid solution, followed by heating for 10 min at 110°C; C) for carbohydrates and polyols with thymol - sulfuric acid, followed by heating for 15-20 min at 120°C. Image-based evaluation in white light and UV 366 nm. The most active bands were also characterized by ATR-FTIR (= attenuated total reflectance – Fourier-transformed infrared) spectroscopy.

J. Planar Chromatogr. 34, 345-351 (2021). HPTLC of Croton tiglium extracts on silica gel with toluene - ethyl acetate 9:1, toluene - ethyl acetate - methanol 6:4:1, toluene - ethyl acetate 3:2, toluene - ethyl acetate - methanol 4:6:1. Detection by spraying with anisaldehyde - sulfuric acid reagent. Identification of steroids and terpenoids on silica gel with toluene - ethyl acetate 9:1 and detection by spraying with the Liebermann‒Burchard reagent, followed by heating at 100 °C. Acidic compounds were analyzed on silica gel with toluene - ethyl acetate 9:1, followed by spraying with bromocresol green reagent. Coumarins were analyzed on silica gel with toluene - ethyl acetate - methanol 4:6:1, followed by spraying with alcoholic potassium hydroxide reagent and visualization under UV light at 366 nm.

Bioorg. Chem. 88, 102947 (2019). HPTLC of pentylcurcumene in the aerial part of Geophila repens on silica gel with benzene - methanol 3:1. Quantitative determination by absorbance measurement at 254 nm. The hRF value of pentylcurcumene was 50. Linearity was between 100 and 500 ng/zone. The intermediate precision was below 2 % (n=3). The LOD and LOQ were 16 and 49 ng/zone. Recovery was between 97.2 and 98.7 %.

J. Planar Chromatogr. 34, 113-120 (2021). HPTLC of thymol (1), carvacrol (2), α-terpineol (3), t-anethole (4), safrole (5), p-anisaldehyde (6), fenchone (7), quercetin (8), kaempferol (9) and quercitrin (10) in common thyme essential oil, star anise essential oil and acerola fruit extract on silica gel with toluene - ethyl acetate 17:3 and methanol - water 1:1. The hRF values for (1) to (10) were 24, 22, 3, 79, 83, 21, 46, 53, 27, 18 and 44, respectively. The OPLC methods were  time- and solvent-saving in comparison with conventional TLC.

Planta Med. 85(3), 195-202 (2019). The dichloromethane fraction of an ethanolic extract from Gloeophyllum odoratum sporocarp (Gloeophyllaceae, Basidiomycetes) was submitted to a multistep purification process (conventional, flash and supercritical fluid column chromatography). At each step, fractions were monitored on TLC silica gel with dichloromethane – methanol – water 40:4:1. Detection under white and UV light after derivatization with vanillin sulfuric acid 5 % in methanol and heating. Eight triterpenes were isolated for further identification: eburicodiol, gloeophyllins B and K, hydroxylanosterol, trametenolic acid B (all five from the lanostane type), gloeophyllins A and L (C‑nor-D-homoergosteroid type), and ergosterol peroxide (ergostane type).

Heliyon 6(8), e04654 (2020). HPTLC of ethanolic extracts of three algae (100µg/band) on silica gel, along with carotenoid standards (10µg/band), developed with toluene – acetone 7:3. Detection under white light. Carotenoids appeared orange or yellow, chlorophylls green, pheophytins dark khaki. Carotenoid patterns of the algae were very different depending on the family: red alga Eucheuma denticulatum (Solieriaceae) contained mainly zeaxanthin and lutein (hRF 44) and β-carotene (hRF 88), but also β-cryptoxanthin (hRF 69-71) and fucoxanthin (hRF 39); brown alga Sargassum polycystum (Sargassaceae) contained mainly fucoxanthin, and also cryptoxanthin; green alga Caulerpa lentillifera (Caulerpaceae) contained mainly zeaxanthin, but also astaxanthin (hRF 61) and canthaxanthin (hRF 77) in smaller amounts. Separately, HPLC-MS was used to confirm and quantify these compounds, which was necessary for carotenoids with similar hRF values: zeaxanthin and lutein (hRF 44), and β-carotene and lycopene (hRF 88).

Planta Med. 84(18), 1348-1354 (2018). A subfraction (obtained through liquid-liquid partition and column chromatography) of the ethanolic extract of whole Vernonia cinerea plants (Asteraceae, subf. Cichorioideae) was further fractioned by reverse-phase SPE (solid-phase extraction) followed by preparative TLC on silica gel layer (eluent not given). For verification, zones were detected by spraying with anisaldehyde solution with 10 % sulfuric acid, followed by heating at 100 °C. Further purification by reverse-phase HPLC allowed the isolation of 6 hirsutinolide-type sesquiterpenoids (all with a oxacyclonane forming an ether bridge), including vernolides A and B.

Planta Med. 84(18), 1340-1347 (2018). Preparative TLC on silica gel for the final purification of 15 phenylethyl-chromenone derivatives (including 3 with epoxide functions), 4 sesquiterpenoids (neopetasane, dehydrokaranone, dioxoselinene, ligudicin C) and a steroid (ergostatetraenone), all isolated through multi-step column chromatography from the ethyl-acetate fraction of a methanolic extract of Aquilaria sinensis resinous wood (Thymelaeaceae). For each compound, mobile phase, RF value and yield are given.