CBS 85, 10-11 (2000) HPTLC-AMD of water samples on silica gel with 33-step gradient from acetonitrile - ammonia to dichloromethane and dichloromethane - acetic acid to n-hexane. Detection via Chrom Biodip (Merck) by dipping in bacteria solution (Bacillus subtilis) followed by incubation at 30 °C over night and spraying with MTT-tetrazolium salt reagent followed by incubation at 30 °C for 5-30 min. Visual evaluation.

J. Liq. Chromatogr. Relat. Technol. 29, 639-647 (2006). TLC of a novel antibiotic on silica gel with chloroform - methanol - diethyl ether 1:7:2. For detection the TLC plates were placed in bioassay plates and overlaid with Muller-Hinton agar which had been seeded with B. fusiformis.

Pharmazie 63, 61-66 (2008). TLC of 3-[(2,2,6,6-tetramethylpiperidine-4-ylimino)methyl]rifamycin and 3-[(2,2,6,6-tetramethyl-1-oxyl-piperidine-4-ylimino)methyl]rifamycin on silica gel with chloroform - methanol 4:1. Detection under white light.

J. Pharma. Sci. & Res. 2(8), 477-483 (2010). TLC on silica gel with ethyl acetate - glacial acetic acid - methanol - water 10:5:3:2. The hRf value was 51 and 74 for amoxicillin and bromhexine, respectively. Densitometric evaluation at 260 nm (bromhexine) and at 320 nm (amoxicillin). The method was linear in the range of 200-1000 ng/band and 10-30 µg/band for bromhexine and amoxicillin, respectively. The recovery was between 98.1-101.5 %.

J. Planar Chromatogr. 26, 43-55 (2013). TLC of cefditoren pivoxil on silica gel with 1-butanol - acetic acid - water 17:2:1. Quantitative determination by absorbance measurement at 295 nm. The hRf of cefditoren pivoxil was 70. Linearity was in the range of 600-1600 ng/zone. The LOD and LOQ were 40 and 130 ng/zone, respectively. Recovery (by standard addition) was between 97.8 and 101.8 %. Intermediate/interday/intra-day precision was below 2 % (n=3). The method showed comparable results with a validated HPLC method.

J. Planar Chromatogr. 27, 392-397 (2014). HPTLC of (1) rifampicin, (2) isoniazid, and (3) pyrazinamide in a dosage tablet on silica gel with ethyl acetate – acetone – methanol – glacial acetic acid 18:5:5:2. Quantitative determination by absorbance measurement between 200 and 800 nm. The hRF values of (1) to (3) were 25, 44 and 74, respectively. Linearities were in the range of 250-600 ng/zone for (1), 125-300 ng/zone for (2) and 625-1500 ng/zone for (3). The intermediate precision was below 2.3 % (n=6) . Mean recoveries for (1) to (3) were 100.0, 98.6 and 99.4 %, respectively.

J. Planar Chromatogr. 30, 219-224 (2017). HPTLC of 18 derivatives obtained by substitution of chlorine atom at C7 or/and C6 in 6,7-dichloro-5,8-quinolinedione on RP-18 with a mixture of acetone and _x000D_water solution of buffer Tris (pH 7.4) in different volume compositions ranged from 30 % to 85 % in 5 % increments. The correlation between experimental and calculated values of lipophilicity was analyzed.

Phytochem. Anal. 30, 121-131 (2019). HPTLC bioautography of essential oils of oregano, rosewood and cumin on silica gel with ethyl acetate – petroleum ether 1:19 for cumin, ethyl acetate – petroleum ether 1:9 for oregano and ethyl acetate – petroleum ether 1:3 for rosewood. 2D resolution by eluting for a second time along the orthogonal axis. TLC-direct bioautography by spraying with a bacterial suspension of methicillin‐sensitive S. aureus NCTC 1298 (MSSA), methicillin‐resistant S. aureus NCTC 12497 (MRSA), E. coli NCTC 8003, ciprofloxacin‐resistant E. coli (clinical isolate), P. aeruginosa NCTC 6749, P. aeruginosa (clinical isolate), vancomycin‐sensitive Enterococcus faecium and vancomycin‐resistant E. faecium NCTC 12202, followed by incubation at 37 ºC for 4 h. Antimicrobial fractions were further characterized by NMR spectroscopy, GC-MS and LC-MS.