Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

  • Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
  • Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
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Registered users can create a tailor made PDF of selected articles throughout CCBS search – simply use the cart icon on the right hand of each abstract to create your individual selection of abstracts. You can export your saved items to PDF by clicking the download icon.

      93 082
      Validated procedure for determination of Monensin-Na in animal feed
      M. WERTHER* (*Institut für Tierarzneimittel GmbH, Berliner Allee 317-321, D-13088 Berlin, Germany, margit.werther@camag-berlin.de)

      CBS 89, 10-11 (2002). HPTLC on silica gel with 1) ethyl acetate - chloroform 1:1 over 30 mm followed by drying at room temperature for 15 min and 2) ethyl acetate - chloroform 9:1 over 50 mm in horizontal development chamber. Detection by dipping in anisaldehyde - sulfuric acid reagent followed by heating at 60 °C for 5 min. Quantitative determination by absorbance measurement at 440 nm. Precision (n=6 at 100 mg/kg feed) is found to be 2.7 %.

      Classification: 28a
      99 069
      Thin-Layer Chromatography - direct bioautography of flumequine residues in milk
      I. M. CHOMA (Department of Chromatographic Methods, University of M. Curie-Sklodowska, M. Sklodowska sq. 3, Lublin 20-031, Poland; ichoma@hermes.umcs.lublin.pl)

      J. Liq. Chromatogr. Relat. Technol. 29, 2083-2093 (2006). TLC of flumequine (9-fluoro-6,7-dihydro-5-methyl-1-oxo-1H,5H-benzo[ij]quinolizine-2-carboxylic acid) on silica gel in a sandwich chamber with dichloromethane - methanol - 2-propanol - 25 % aqueous ammonia 3:3:5:2. The plates were developed to the top and then continuously for 1 h. Bioautography with nutrient medium and Bacillus subtilis spore suspension. After incubation the plates were sprayed with MTT-solution.

      Classification: 28a
      101 039
      A newly developed assay for the quantitative determination of antimicrobial (anticyanobacterial) activity of both hydrophilic and lipophilic test compounds without any restriction
      R. VOLK (Department of Pharmaceutical Biology, Pharmaceutical Institute, University of Kiel, Kiel, Gutenbergstrabe 76, 24118 Kiel, Germany, volk@pharmazie.uni-kiel.de)

      Microbiol. Res. 163, 161-167 (2008). Test solutions containing 0.25 to 64 µg of norharmane, quinine, and tetracycline (as bases and hydrochloride salts) were applied as 10 mm bands on silica gel plates. After coating the plate with a concentrated suspension of the living cyanobacterial test organism (spraying or dipping), it was kept moist in a TLC chamber at 27 ºC for 1 to 2 days under continuous illumination (25 - 30 µmol photon/m2s). Cytotoxic concentrations of a test compound resulted in an easily recognizable regional decolourisation of the test organism.

      Classification: 28a
      108 061
      The visualizing agents for selected quinolones and fluoroquinolones
      K. BOBER (Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Silesia, 4 Jagiellonska Street, 41-200, Sosnowiec, Poland, bober@sum.edu.pl)

      J. Liq. Chromatogr. Relat. Technol. 32, 3049-3055 (2009). HPTLC of cinoxacin (1), pipemidic acid (2), ofloxacin (3), and pefloxacin (4) on silica gel with buffer solution (pH 5.5) - methanol 4:1 and acetonitrile - water - acetic acid, 3:20:2. Detection by dipping into various visualization agents. The detection limits for (1) to (4) were 0.1, 0.1, 0.5, and 0.75 µg/zone, respectively. Best detection conditions for (1) and (2) were obtained by dipping in Janus blue and immediate evaluation, and for (3) and (4) by dipping in Cresol red followed by heating at 120 ºC for 10 min for (3) and (4).

      Classification: 28a
      112 066
      Development and validation of a thin-layer chromatographic–densitometric method for the analysis of ciprofloxacin hydrochloride tablets
      B. NYAMWERU, E. KAALE*, V. MANYANGA, M. CHAMBUSO, T. LAYLOFF (*Pharm R&D Laboratory, School of Pharmacy, Muhimbili University of Health and Allied Sciences, P.O. Box 65013, Dar es Salaam, Tanzania, elia.kaale@lycos.com)

      J. Planar Chromatogr. 26, 370-374 (2013). HPTLC of ciprofloxacin in tablets on silica gel with acetone - water - ammonia 30:3:5. Quantification by absorbance measurement at 280 nm. The hRf of ciprofloxacin was 27. Linearity was in the range of 250-600 ng/zone. Recovery was in the range of 96-101 %. Intermediate/interday/intra-day precision was below 2 %.

      Classification: 28a
      115 023
      Effect-directed analysis of cold-pressed hemp, flax and canola seed oils by planar chromatography linked with (bio)assays and mass spectrometry
      S. TEH (Teh Sue Siang), Gertrud MORLOCK* (*Justus Liebig University Giessen, Institute of Nutritional Science, Chair of Food Science, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany, gertrud.morlock@ernaehrung.uni-giessen.de)

      Food Chem. 187, 460-468 (2015). HPTLC-direct bioautography of bioactive compounds in the extracts of cold-pressed hemp (1), flax (2) and canola (3) seed oil on silica gel with toluene - ethyl acetate - formic acid - water 15:30:5:3 for (2) and toluene - ethyl acetate - acetic acid 80:25:4 for (1) and (3). HPTLC-DPPH scavenging activity was determined by dipping into a methanolic DPPH solution, followed by drying for 90 s in the dark and heating at 60 °C for 30 s. The hRF values of dominant radical scavenging zones were in the range of 75-85 for (1), 70-90 for (2) and 64 and 95-100 or (3). HPTLC-antimicrobial Aliivibrio fischeri assay allowed the determination of major antimicrobial zones at hRF 40-49 and 55-66 or (1), 23, 45 and 60 for (3) and 95 for (2). Additional effect-directed analyses employing acetylcholinesterase (AChE) assay, planar yeast estrogen (pYES) bioassay and Bacillus subtilis bioassay as well as subsequent HPTLC-ESI-MS allowed targeted characterization of bioactive compounds.

      Classification: 7, 8a, 28a
      119 078
      High-performance thin-layer chromatographic method for the estimation of benzyl isothiocyanate in Salvadora persica root extract and dental care herbal products
      M. ABDEL*, P. ALAM, M. HAMED, M. AYMAN (*Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21215, Egypt, mpharm101@hotmail.com)

      J. Planar Chromatogr. 30, 211-215 (2017). HPTLC of benzyl isothiocyanate in the root of Salvadora persica on silica gel with n-hexane ‒ ethyl acetate 9:1. Quantitative determination by absorbance measurement at 191 nm. The hRF value for benzyl isothiocyanate was 61. Linearity was between 100 and 600 ng/zone. The intermediate precision was below 2 % (n=3). Recovery ranged from 97.6 to 99.5 %.

      Classification: 28a
      122 067
      Thin-layer chromatography–contact bioautography as a tool for bioassay-guided isolation of anti-Streptococcus mutans compounds from Pinus merkusii heartwood
      A. SAKUNPAK*, L. SUEREE (*Department of Pharmacognosy, College of Pharmacy, Rangsit University, Pathum Thani 12000, Thailand, apirak.s@rsu.ac.th)

      J. Planar Chromatogr. 31, 355-359 (2018). HPTLC bioautography of anti-Streptococcus mutans compounds in Pinus merkusii heartwood on silica gel with n-hexane – ethyl acetate 7:3. Plates were placed onto BHI agar plates inoculated with S. mutans, followed by incubation at 37 °C for 24 h. The hRF values of bioactive compounds were 65 and 85. Further purification and analysis of active zones allowed the identification of dehydroabietic acid, isopimaric acid, and abietic acid.

      Classification: 28a