J. Chromatogr. A 787, 227-233 (1997). Examination of the influence of temperature and mobile phase composition on retention of a-, b-, and g-cyclodextrins and two macrocyclic antibiotics. Measurement of RF values of the solutes at temperature from 5 to 60°C. Estimation of the change of enthalpy (DH°) and entropy (DS°) from linear Van't Hoff plots. Discussion of temperature as useful parameter for designing an enantioselective chromatographic system in which Rf values of the chiral phases modifier should be equal to 0 or 1.

J. Planar Chromatogr. 14, 126-129 (2001). HPTLC of fleroxacin, sparfloxacin, and cinoxacin on silica gel with dichloromethane - isopropanol - 25% NH3 4:5:2. Quantitation by densitometry at 254 nm. Information on the range of linearity, sensitivity of detection, relative standard deviation, and recovery is given.

J. Planar Chromatogr. 17, 84-88 (2004). OPLC of trans-resveratrol on silica gel with chloroform - methanol 10:1 after preconditioning of the plates for 3 h at 120 °C. Detection by immersion of the dried plates in a bacterial suspension (Pseudomonas savastanoi pv. phaseolicola race 6) for 20 s and MTT staining (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide; 80 mg MTT and 100 mg Triton X-100 in 100 mL water) either after a short draining period or after overnight incubation; after staining the time for evaluation varied (e. g. from 1 h to 6 - 9 days or more). First results of the investigation of a complex bioautographic system with special emphasis on the direct effect of the antibiotic trans-resveratrol (fitoalexin) and the function of formaldehyde in relation to antibiotics.

J. Liq. Chromatogr. Relat. Technol. 30, 2231-2244 (2007). HPTLC of eight cephalosporins (cefaclor, cefoperazone, cefazolin, cefotaxime, cefoxitin, cefuroxime, cephalotin, and p-chlorophenacyl cephalothin) on silica gel with diisopropyl ether - toluene - ethyl acetate - 80 % formic acid 1:4:13:2 in a sandwich chamber. Also HPTLC on diol-, amino-, and cyano-modified silica gel. Detection under UV light at 254 nm.

J. AOAC Int. 94, 1567-1572 (2011). TLC of flumequin as standard on silica gel using various incubation times. After incubation, the plates were sprayed with 0.2 % aqueous MTT solution and incubated for 0.5 h at 37 °C. The regression coefficients were 0.9977 and 0.9968, respectively, for intraday and interday curves. The calibration curves showed good linearity in the range of 5-500 ng (0.5-50.0 µg/mL). The established LOD of flumequine was 5 ng/zone (0.5 µg/mL). One drop of triton X-100/10 mL aqueous MTT solution was found to enhance the intensity of the color.

J. Planar Chromatogr. 26, 470-474 (2013). TLC-bioautography of antimicrobial metabolites in the extracts of endophytic fungus Xylaria sp. on silica gel with toluene - diethyl ether - acetic acid 1:1:1. The plates were transferred to sterile Petri dishes and overlaid with Mueller Hinton (for Escherichia coli and Vibrio parahaemolyticus), Brain Heart Infusion (for Staphylococcus aureus and Listeria monocytogenes) medium containing 0.65 % agar incorporating 1 mg/mL 2,3,5-triphenyltetrazolium chloride, and Sabouraud Dextrose Agar medium (for Candida albicans and Aspergillus niger) inoculated with 1 % standardized microbial inocula. For bacteria plates were incubated for 24 h at 37 °C. For fungi, plates were incubated for 48-72 h at 25 °C and the agar surface was sprayed with a 5 mg/mL solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and inhibition zones were detected as clear spots against a red and purple background.

J. AOAC Int. 98, 850-856 (2015). Review of the development of planar chromatography-direct bioautography methodology, with special emphasis on its use as a biomonitoring system. HPTLC of cis-spiroether (1), trans-spiroether (2), alpha-bisabolol (3), bisabolol oxides (4) and the fluorescent coumarin derivatives herniarin (5), and umbelliferone (6) in chamomile extracts on silica gel with chloroform - acetone 99:1. Detection by dipping into vanillin–sulfuric acid reagent (40 mg vanillin, 10 mL ethanol and 200 mL concentrated sulfuric acid) followed by heating at 110 °C for 5 min. Bioassays were performed by the direct bioautographic system using a Gram-negative test bacteria (Xanthomanas euvesicatoria), the luminescence gene-tagged Arabidopsis pathogen (Pseudomonas syringae pv. maculicola) and the luminescent marine bacterium (Aliivibrio fischeri) as well as a Gram-positive soil bacterium (Bacillus subtilis).

J. Liq. Chromatogr. Relat. Technol. 40, 22-286 (2017). HPTLC of clarithromycin (1), azithromycin (2), and amodiaquine (3) and artesunate (4) on silica gel with methanol – ethyl acetate – concentrated ammonium hydroxide 40:10:1 for (1) and (2) and acetone – water – concentrated ammonium hydroxide 40:7:2 for (3) and (4). Detection by heating for 15 and 30 min, respectively, for (1) and (2) at 160 °C to activate fluorescence quenching. Naturally fluorescence quenching zones of (3) were scanned at 254 nm and (4) after heating the plate for 5 min at 180 °C to activate fluorescence quenching.