J. Agric. Food Chem. 39, 386-391 (1991). TLC of maduramicin and metabolites on silica with hexane - acetone 1:1, toluene - ethyl acetate - methanol 7:10:3, ethyl acetate - ether - ethanol - triethanolamine - water 200:200:6:8:7. Detection by radioactive image scanner, spraying with methanolic vanillin - sulfuric acid solution, followed by heating at 100°C for 10 min. Preparative TLC on RP-18 with methanol - water 8:2; also 2-dimensional TLC with hexane - acetone 1:1, 2nd dimens. methanol - toluene - ethyl acetate 3:7:10.

Biomed. Chromatogr. 6, 196-197 (1992). TLC of tetracycline and amino glycopeptide antibiotics on silica with ethanol - acetic acid - water 10:6:6, and butanol - formic acid - water 6:5:7. Detection by exposing to iodine vapor.

Gesundheitshilfe Dritte Welt - German Pharma Health Fund e.V., 1994, 108 p. Description of simple and technically undemanding TLC methods for the identification of some compounds from the Essential Drug List, (amoxicillin, ampicillin, benzylpenicillin, chloroquine, various tetracyclines), corticosteroids (dexamethasone, betamethasone, prednisolone, hydrocortisone, hydrocortisone acetate), acetylsalicylic acid, paracetamol, ibuprofen, metamizole, albendazole, and mebendazole. TLC on silica or cellulose with mobile phases as simple and non-toxic as possible. Detection under UV or with platinate solution, Dragendorff's reagent, iodine vapor, ethanolic ninhydrin solution etc.

Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 69-73 (1997). HPTLC of neomycin B, C and neamin (neomycin A) on silica with 1. methanol - acetone - 5% acetic acid 12:15:13 and 2. methanol - acetone - 25% NH3 12:15:13. Postchromatographic derivatization with fluorescamine, stabilization with paraffin/triethanolamine/methyl ethyl ketone.

Part IV: Quantitation and evaluation of penicillins. J. Planar Chromatogr. 12, 180 - 185 (1999). TLC of ampicillin, piperacillin, penicillin, mezlocillin, ticarcillin and oxacillin (direct sample determination) on RP hydrocarbon-impregnated HPTLC plates without solvent elution. The samples remained as a single spot centered about the point of application thereby facilitating direct quantitation by densitometry at different wavelengths. Detection limit 0.1 ng. Quantitation by densitometry in the range of 200 to 300 nm. This technique, direct sample determination (DSD), results in a higher sample throughput in reduced time with all samples being determined under the same conditions as the standards, without sample elution.

J. Liq. Chrom. & Rel. Technol. 25, 1579-1587 (2002). TLC and HPTLC of flumequine (9-fluoro-6,7-dihydro-5-methyl-1-oxo-1H,5H-benzo[ij]quinolizine-2-carboxylic acid) on silica gel with 0.05 M citric acid - methanol -2-propanol 1:3:1 or chloroform - acetone 9:1. Bioautography was performed by immersing the developed TLC plates briefly in the microorganism solution and incubated overnight at 28°C. Then the plates were sprayed with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)-solution and incubated for about 30 min. Yellow inhibition zones were seen against a purple background. Sensitive, simple and cheap separation and determination procedure.

J. Planar Chromatogr. 19, 104-108 (2006). HPTLC and TLC of ciprofloxacin and enrofloxacin on silica gel with dichloromethane - methanol - 2-propanol - 25% aqueous ammonia 3:3:5:2. The plate was developed in a DS chamber to the top and the separation chamber was then uncovered for about 1 cm to enable the solvent to evaporate. In this way the plate was developed continuously for 2 h. Bioautography by immersion of the plate in a microorganism solution (Bacillus subtilis), incubation for 22 h at 37 °C. Visualization by spraying with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) solution and leaving for approximately 30 min at room temperature.

Food Addit. Contam. 24, 351-359 (2007). Inter-laboratory validation of the analytical method (published in the SIMBAG-FEED report 4.6) based on TLC coupled to bio-autography for the detection of tylosin, spiramycin and virginiamycin in feeding-stuffs for poultry, pig, cattle and calf. The detection limit of spiramycin was 2 mg/kg and the method has a target concentration of 1 mg/kg for tylosin and virginiamycin. The method showed high specificity and offers the possibility for screening before LC/MS analysis.