J. China Pharm. Univ.(Zhongguo Yaoke Daxue Xuebao) 22, 241-243 (1991). TLC of ribostamycina and neamine on silica with chloroform – methanol – NH3 – acetone 2:3:2:2. Detection by spraying with 0.03% ninhydrin. Quantification by densitometry at 535 nm.

J. Planar Chromatogr. 7, 159-162 (1994). HPTLC of oxolinic acid on silica impregnated with 0.1 M K2HPO4 with chloroform - acetone 9:1; of erythromycin on silica with ethyl acetate - acetic acid - water 6:2:2. Visualization by derivatization with 4-methoxybenzaldehyde; quantification by densitometry (absorbance at 517 nm). HPTLC of oxytetracycline on silica treated by dipping into an aqueous solution of 10% EDTA with dichloromethane - methanol - water 59:35:6. Scanning by fluorescence at 366/>400 nm or by absorbance at 254 or 280 nm.

J. AOAC Int. 79, 375-379 (1996). TLC of oxytetracycline or chlorotetracycline in animal feeds in the presence of 11 other drugs (3 nitrofurans, 2 macrolide antibiotics, 3 sulfonamides, 2 coccidiostatics, 1 antibacterial growth promoter) on silica with chloroform - methanol 10:1. Detection under UV 254 and 366 nm. Quantification after microbiologically elution by the agar diffusion method. Limit of quantification 0.625 ppm, recovery 92.9 %, CV 2.3 - 4.4 %.

J. AOAC Int. 81, 51-56 (1998). TLC of 6 sulfonamides (sulfadimidine, sulfadoxine, sulfadiazine, sulfadimethoxine, sulfathiazole, sulfamerazine) on silica gel with concentration zone with mixtures of NH3 in methanol and dichloromethane and a 3-step TLC method acc. to L.S.G. van Poucke et al., J. Chromatogr. Sci. 29, 423-427 (1991). Detection after drying by dipping in a 0.01% fluorescamine in acetone solution and observation under UV 366 nm. Semiquantitative determination by visual comparison of the spots against spiked samples.

J. Planar Chromatogr. 14, 350-354 (2001). TLC of troleandomycin, tylosin, rifamycin B, vancomycin, erythromycin, and rifampicin on polyamide 11 plates with five binary solvent mixtures methanol - water, ethanol - water, propanol - water, acetonitrile - water, and tetrahydrofuran - water. Detection by spraying with anisaldehyde - methanol - acetic acid - orthophosphoric acid - sulfuric acid 1:100:10:10:5 and heating at 110°C for 10 min.

J. Planar Chromatogr. 18, 423-426 (2005). HPTLC of antibiotics (sulfadimidine, sulfadiazine, sulfaguanidine, trimethoprim) on silica gel without chamber saturation in a twin-trough chamber with chloroform - methanol 89:11. Quantification by videodensitometry at 254 nm. Limit of detection was 0.05 µg per spot for sulfadimidine, sulfadiazine, and sulfaguanidine, and 0.1 µg per spot for trimethoprim.

J. AOAC Int. 92, 1068-1075 (2009). TLC and HPTLC of six antibiotics (amikacin, gentamicin, kanamycin, neomycin, netilmicin, and tobramycin) in pharmaceutical preparations on silica gel with methanol - 25 % ammonia - chloroform 3:2:1. Detection by derivatization with 0.2 % ethanolic ninhydrin reagent. Quantitative determination by absorbance measurement at 500 nm. The limit of detection and quantification was 250 and 500 ng/zone for amikacin, 500 and 1000 ng/zone for kanamycin, 480 and 800 ng/zone for neomycin, 380 and 630 ng/zone for netilmycin, 480 and 800 ng/zone for tobramycin and 1000 and 1650 ng/zone for gentamicin. Precision was good with %RSD of 0.3-0.6 %.

J. AOAC Int. 94, 735-742 (2011). HPTLC and TLC of ofloxacin on silica gel with ethanol - conc. ammonia 4:1 in a horizontal chamber. Quantitative determination by fluorescence measurement at 313 nm. Simulated samples at a residue level of 1 mg/m² were prepared by spreading the calculated amount of ofloxacin solution on 1, 5, and 10 dm² stainless steel surfaces. The hRf of ofloxacin was 56. The mean recovery (n = 6) was 88.6-95.3 % with a CV of 3.8-4.9 %. The LOD was 0.6 ng/zone and the LOQ was 2 ng/zone, but it was shown that these can be lowered by immersion of the developed plate into a solution of liquid paraffin - n-hexane 1:2 to approximately 0.3 and 0.9 ng/zone. The repeatability (system precision) was 4.2 % for 2 ng, and 3.7 % for 20 ng. The recovery was between 88.6-95.3 %.