Part III: Quantitation and evaluation of cephalosporins. J. Planar Chromatogr. 12, 114-119 (1999). Scanning densitometry for direct quantitation of cefoxitin, cefpirome, cefotaxime, cefoperazone, cefepime, ceftazidime, ceftizoxime, ceftriaxone, cefuroxime, cefotetan, cephalothin, and cefazolin on reversed-phase hydrocarbon-impregnated HPTLC silica gel plates without prior solvent elution. The samples remained as a single spot centered about the point of application, thereby facilitating direct quantitation by densitometry at different wavelengths.

J. Planar Chromatogr. 15, 187-191 (2002). TLC of flumequine and doxycycline on silica gel with and without a concentrating zone (after predeveloping with hexane and acetone) with 0.05 M citric acid - methanol - 2-propanol 1:3:1. Detection under UV 366 and 254 nm and by densitometry at 360 nm (for doxycycline) and at 325 nm (for flumequine). Bioautography by dipping the developed plates in a nutrient medium inoculated with Bacillus subtilis spore suspension and incubated overnight at 28°C. The plates were then sprayed with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) solution and incubated for approximately 30 min.

J. AOAC Int. 88, 1555-1561 (2005). HPTLC of chloramphenicol on silica gel in a horizontal developing chamber (36 applications per plate) using n-hexane -ethyl acetate 7:13. Quantitative determination by absorbance measurement at 280 nm. Mean recovery was 95.8 %, and the coefficient of variation was 5.8 %. The detection limit was 3 ng, and the quantitation limit 10 ng.

Anal. Chem. 81, 3858-3866 (2009). HPTLC of microcystin LR and nodularin on silica gel with 1-propanol - ethyl acetate - water 3:5:2 with 5 % acetic acid. Detection and quantification by UV spectroscopy at 232 nm and direct identification of separated analytes on the HPTLC plate by IR-MALDI-o-TOF MS. The detection limit was 3-5 ng/zone. For detection of peptides, plates were cut and sprayed with a solution of p-anisaldehyde, followed by heating at 105 °C until purple-blue peptide spots appeared.

Food Control. 68, 310-329 (2016). Review of the methodologies to determine the occurrence of aflatoxin M1 (AFM1) and the fate of AFM1 during processing of milk and dairy products, such as yoghurt and cheeses, since 1996 until today. The review describes the application of TLC and HPTLC in raw and pasteurized milk, feta cheese, yoghurt, white cheese, ice cream and butter.

J.A.O.A.C. 67, 611-612 (1984). TLC of aflatoxin B1 and ochratoxin A on silica. Clean-up development with benzene - hexane 3:1, followed by toluene - ethyl acetate - formic acid 60:30:15. Also ethyl acetate - formic acid 9:1, hexane - methanol - acetic acid 18:1:1. Detection by UV 375 nm. Detection limits 5-7 mg aflatoxin B1 and 20 mg ochratoxin A/kg.

(Hungarian). (Zearalenone formation of F-2 toxin producing mould strains in different substrata.) Investigation of 21 mould strains belonging to seven different fusarium species on their F-2 toxin formation wheat, maize and rice substrate at different incubation temperatures. TLC on silica with chloroform - ethanol 97:3. Detection at UV 254 nm and 366 nm or by spraying with 2 % 4-methoxy-benzene - diazonium fluoroborate solution. Quantification by GC.

J. Chromatogr. 356, 359-366 (1986). HPTLC of phomenone on silica with 1) chloroform - methanol 85:15, 2) ethyl acetate - hexane 9:1. Detection under UV at 254 nm and by spraying with first 10 % sulfuric acid, then 3 % phosphomolybdic acid and heating for 10 min at 110 °C. Quantification by densitometry at 240 nm. Detection limit 10 ng. Comparison to HPLC.