Anal. Chim. Acta 632 (2), 168-180 (2009). Ochratoxins and aflatoxins are the most significant mycotoxins and there has been a broad range of research. However, it is impossible to use one standard technique for the analysis because of the various structures of mycotoxins. The review discusses existing analytical and detection techniques, such as 1) sample pre-treatment methods like liquid-liquid extraction, supercritical fluid extraction, or solid phase extraction; 2) separation methods such as TLC, HPLC, GC, and CE and 3) other methods such as ELISA. The practical requirements for high-sensitivity analysis and the need for a specialist laboratory setting create challenges for routine analysis. There are a number of methods used, but there is no single technique that stands out above the rest, although HPLC-MS is popular. Discussion of further currents trends, advantages and disadvantages and future prospects of these methods.

J. Chil. Chem. Soc. 62, 3435-3437 (2017). HPTLC of deoxynivalenol in wheat crop samples on silica gel with toluene – ethyl acetate – formic acid 6:3:1. Detection by spraying with 10 % aluminium chloride in ethanol – water 1:1, followed by heating at 120 °C for 10 min. Quantitative determination by fluorescence measurement at UV 366/>400 nm. The identity and purity of zones were confirmed by direct mass spectrometry on the plate using a TLC/MS elution head-based interface. Linearity was between 8-120 ng/zone. LOD and LOQ were 0.05 and 0.19 mg/kg, respectively. Average recovery rate was 90.1 %.

(Thin-layer chromatography and high pressure liquid chromatography in mycotoxin analysis.) Z. Lebensm. Unters. Forsch. 178, 79-80 (l984). TLC and HPTLC methods support each other; the TLC method is preferred when the samples to be analyzed are of variable origin while HPLC is attractive when samples are uniform or at least similar.

J.A.O.A.C. 67, 611-612 (1984). TLC of aflatoxin B1 and ochratoxin A on silica with a) benzene - hexane 3:1, b) toluene - ethyl acetate - formic acid 60:30:15. Detection under UV at 375 nm. Quantification by spectrophotometry. Detection limits 5-7 mg/kg for aflatoxin B1 and 20 mg/kg for ochratoxin A.

J. Microchem. 32, 170-172 (1965). TLC of aflatoxin M1 on silica with ether - methanol - water 96:3:1. Quantification by densitometry at 439 nm. Detection limit 50 ppt.

J. Liquid Chrom. 9, 1591- 1602 (1986). TLC of 18 trichothecenes on silica RP-18 with ethanol - water - acetic acid 65:35:1 + 0.5% NaCl; Visualization with anisaldehyde and chromotropic acid spray reagents. GC and TLC methods have a greater sensitivity than HPLC for the underivatized mycotoxins.

Phytochemistry 28, 73-75 (1989). TLC of stemphyperylenol, stemphyltoxin II, alterperylenol and altertoxin I on silica with chloroform - methanol 9:1. Detection under UV. Purification on silica with chloroform - methanol 9:1 or toluene - ethyl acetate 1:1.

and in infected plants by high performance liquid chromatography and thin-layer chromatography. Phytochemical Analysis 2, 87-89 (1991). TLC and HPTLC of various cytochalasins on silica with chloroform - methanol 92:8 or chloroform - isopropanol 9:1. Also reversed phase TLC on RP-18 with acetonitrile - water 60:40. Detection by UV or by spraying with 10% H2SO4, followed with 3% phosphomoybdic acid in methanol. Method suitable for a rapid detection of the toxins in host plants.