Cumulative CAMAG Bibliography Service CCBS
Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.
The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:
- Full text search: Enter a keyword, e.g. an author's name, a substance, a technique, a reagent or a term and see all related publications
- Browse and search by CBS classification: Select one of the 38 CBS classification categories where you want to search by a keyword
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J. Planar Chromatogr. 23, 265-269 (2010). TLC of the epimers of cefaclor on silica gel (impregnated with beta-cyclodextrin by development with 1:9 aqueous beta-cyclodextrin solution - methanol) with chloroform - ethyl acetate - glacial acetic acid - water 4:4:4:1 with chamber saturation. Chromatograms were developed at 5 °C and dried at room temperature. Quantitative determination by absorbance measurement at 274 nm. The limit of detection was 0.24 and 0.27 µg/band for the epimers at hRf 26 and 33, respectively. The limit of quantification was 0.74 and 0.83 µg/band, respectively. The recovery was between 100.0 and 100.8 % and the precision between 0.7 and 1.7 %.
J. Planar Chromatogr. 26, 409-416 (2013). TLC bioautography of tylosin (1), spiramycin (2), and erythromycin (3) residues in the meat of Egyptian buffaloes on silica gel with methanol - ethyl acetate - acetone 5:3:2. The hRf values of (1) to (3) were 80, 41 and 20, respectively. Linearity was between 15-120 ng/zone for (1), 50-200 ng/zone for (2) and 1-20 ng/zone for (3). LOD for (1) to (3) were 12, 45 and 2 ng/g, respectively. Recovery (by standard addition) was found to be 84.2-92.2 %. Precision as %RSD was below 7.6 %. Bioautography was performed after development according to the method by J. Michard et al. (Method protocol for the detection of tylosin, spiramycin and virginiamycin in animal feedingstuffs by thin layer chromatography. SIMBAG-FEED Report 4.6. RIKILT, 2005). TLC plates were dried at 60 °C for 30 min in a petri dish and then covered to a thickness of 1.5 mm with agar medium containing the bacterial suspension. After incubation over night at 35 °C about 5 mL of 2,3,5-triphenyltetrazolium chloride solution (0.1 g in 100 mL water) was poured on the medium and re-incubated for some minutes. The inhibition zones were measured and their hRf values were compared with those of antibiotic standards. Detection was considered positive if the hRf value of the sample’s inhibition zone is ±5 % of the standard.
Phytochemistry 105, 68-78 (2014). HPTLC bioautography of antifungal compounds in the roots of Morinda tormentosa on silica gel with petroleum ether - ethyl acetate 1:1, detection at UV 254 and UV 366 nm. The plates were incubated overnight at 37 ºC, after solidification of 20 mL of C. albicans suspension distributed over the plate. Bioautograms were sprayed with an aqueous solution of methyl thiazolyl tetrazolium chloride (2.5 mg/mL), followed by incubation at 37 ºC for 6h. Clear inhibition zones were observed against a purple background.
Microbiol. Res. 186-187, 119-131 (2016). TLC bioautographic analysis of active extracts of the producing strain Natrinema gari QI1 on silica gel with n-butanol – acetic acid – water 3:1:1. The agar media containing the indicator strain Haloferax mediterranei SAG3 was applied directly onto the developed TLC plate and incubated at 40 °C for 7 days. The compound with the most significant inhibition of Haloferax mediterranei was at hRF 29.
J. Planar Chromatogr. 31, 290-296 (2018). HPTLC of sulfadoxine (1), sulfalene (2), and_x000D_ pyrimethamine (3) on silica gel with toluene - ethyl acetate - methanol 100:56:43. Quantitative determination by absorbance measurement at 254 nm. Linearity was between 1 and 3 μg/zone for (1) and (2), and 0.05 and 0.15 μg/zone for (3). LOD was 0.107 ng/zone for (1), 0.04 ng/zone for (2) and 1.62 ng/zone for (3), and LOQ was 0.32 ng/zone for (1), 0.13 ng/zone for (2) and 4.92 ng/zone for (3). The intermediate precision was <4 %. Average recovery was 98.0 % for (1), 97.2 % for (2) and 101.8 % for (3).
J. Chromatogr. 323, 424-428 (1985). TLC on silica with methanol - ethyl acetate 8:2. Use of several lambda-acceptors as spray reagents producin ghighly stable colours for the detection of penicillins on silica layers. 0.5 % p-chloranil in dioxane followed by dimethylformamide (DMF) is recommended due to its sensitivity and stability.
J. Chromatogr. 367, 323-334 (1986). TLC of some rifamycins and steroids on diphenyl-bonded silica with various solvents, including non-aqueous and aqueous binary mixtures. Proposal of a dual retention mechanism. Interactions of the residual silanol groups seem to prevail when using lipophilic solvents as the mobile phase, whereas interactions with bonded aryl. groups dominate when high polarity alcoholic or aqueous mixtures are being used. Diphenyl silica precoated plates, rifamycins, steroids, mobile phase composition
J. Chromtogr. 435, 229-234 (1988). TLC identification on silica with chloroform - acetone - isopropanol 85:10:5, or ether - methanol - water 96:3:1. Detection under UV 365 nm and 254 nm. Confirmation by spraying with 25% sulfuric acid or 5% nitric acid.