Special part: TLC of lipids, vitamins, carotenoids, flavonoids, organic acids, pesticides, insecticides, antioxidants, mycotoxins, surfactants.

J. Chromatogr. 624, 171-194 (1992). Review with 127 references on the title content, including TLC methods for penicillin residues. Discussion of the need to develop sensitive, accurate, and reliable methods to support regulatory programs and emerging techniques suitable to improve sensitivity and usefulness of current chromatographic methods.

Microbiol. Res. 163, 624-632 (2008). TLC of FK506 and FK520 from the fermentation broths of Streptomyces species, on silica gel with isopropyl alcohol – benzene 3:17 or methylene chloride – acetone 2:1. Detection by bioautography with the A. IDR 721 test organism, and also by spraying the plate with cesium sulphate 1 % in sulfuric acid 10 % followed by heating at 120 °C. The hRf of the immunosupressant compounds were 50 or 60, depending on the developing solvent.

J. Planar Chromatogr. 24, 196-200 (2011). Experimental RM values of a series of 3-(4-alkylphenyl)-4-thioxo-2H-1,3-benzoxazine-2(3H)-ones and 3-(4-alkylphenyl)-2H-1,3-benzoxazine-2,4(3H)-ones were obtained by a reversed-phase TLC system. The mobile phase was a phosphate buffer with volume fractions of acetone between 80 and 60 %. TLC of 14 compounds on RP-18 with phosphate buffer pH 7.4 and acetone (80, 75, 70, 65, and 60 %). Detection under UV light. The retention constants RM0 were compared with the partition coefficients calculated using different software products.

Phytochemistry 105, 68-78 (2014). HPTLC of antifungal compounds in Morinda tormentosa on silica gel with dichloromethane - methanol - ethyl acetate 43:6:1. Bioautography with Candida albicans DSY2621 or parent wild type CAF2-1 at 37 ºC overnight, followed by spraying with an aqueous solution (2.5 mg/ml) of thiazolyl blue (methyl thiazolyl tetrazolium chloride) and incubation for 6 h at 37 ºC. Clear inhibition zones were observed against a purple background.

Pharm. Res. 32, 2663-2677 (2015). TLC for the determination of amphotericin B cargo tagged with fluorescein isothiocyanate on silica gel with ethyl acetate - methanol 2:3. Qualitative determination by fluorescence measurement at 485/>538 nm.

Microbiol. Res. 199, 89-97 (2017). HPTLC bioautography of lipopeptide crude extracts from wild-type strain Bacillus subtilis 9407 on silica gel with chloroform – methanol – water 65:25:4. The plates were laid on the surface of a potato dextrose agar containing fungal spores at room temperature for 2 h and then removed. Incubation at 28 °C for 5 days. The hRF value for fengycin was 15.

Acta Veterinaria 32, 97-101 (1984). TLC of monensin, nigericin on silica with chloroform - methanol 9:1. Spraying with vanillin - sulfuric acid solution, followed by heating at 800C for 5 minutes. Scanning by absorbance at 505 nm for monensin, at 510 nm for nigericin. Standard deviation + 0.8 - + 4 %.