XVII. Mycotoxins in foodstuffs. Z. Lebensm. Unters. Forsch. 176, 36-39 (1983). TLC of tenuazonic acid, alternariol, alternariol monomethyl ether, altenuen on silica with a) toluene - ethyl acetate - 90 % formic acid 6:3:1, b) chloroform - methanol 95:5. Detection by UV.

J. Chromatogr. 262, 340-345 (1983). TLC of ethoxyquin on silica with benzene or methanol. Fluorescence enhancement by spraying with Triton X-100 - benzene 1:2. Detection by UV 365 nm. Fluorometry at 366 nm.

J. Agric. Food Chem. 33, 326-330 (1985). TLC of deoxynivalenol on silica with chloroform - acetone - isopropanol 8:1:1. Elution with Eluchrom after spraying a standard with AlCl3 solution and heating at 105 °C for 2 minutes. After evaporation of the eluate MS analysis.

J. Chromatogr. 580, 279-291 (1992). Discussion of the determination methods of amanitins, toxins of aminitic phalloides (Fr.), link mushrooms and related toxins by TLC and other chromatographic techniques. Discussion also of the main chemical and toxicological aspects.

J. Planar Chromatogr. 21, 209-212 (2008). HPTLC of levofloxacon, (-)-(S)-9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid, and amlodipine besylate (as internal standard) on silica gel, prewashed with methanol, with chloroform - methanol - acetic acid 58:39:3 in a twin trough chamber. Quantitation by densitometry at 300 nm (levofloxacin) and 350 nm (internal standard).

J. Planar Chromatogr. 23, 265-269 (2010). TLC of the epimers of cefaclor on silica gel (impregnated with beta-cyclodextrin by development with 1:9 aqueous beta-cyclodextrin solution - methanol) with chloroform - ethyl acetate - glacial acetic acid - water 4:4:4:1 with chamber saturation. Chromatograms were developed at 5 °C and dried at room temperature. Quantitative determination by absorbance measurement at 274 nm. The limit of detection was 0.24 and 0.27 µg/band for the epimers at hRf 26 and 33, respectively. The limit of quantification was 0.74 and 0.83 µg/band, respectively. The recovery was between 100.0 and 100.8 % and the precision between 0.7 and 1.7 %.

J. Planar Chromatogr. 26, 409-416 (2013). TLC bioautography of tylosin (1), spiramycin (2), and erythromycin (3) residues in the meat of Egyptian buffaloes on silica gel with methanol - ethyl acetate - acetone 5:3:2. The hRf values of (1) to (3) were 80, 41 and 20, respectively. Linearity was between 15-120 ng/zone for (1), 50-200 ng/zone for (2) and 1-20 ng/zone for (3). LOD for (1) to (3) were 12, 45 and 2 ng/g, respectively. Recovery (by standard addition) was found to be 84.2-92.2 %. Precision as %RSD was below 7.6 %. Bioautography was performed after development according to the method by J. Michard et al. (Method protocol for the detection of tylosin, spiramycin and virginiamycin in animal feedingstuffs by thin layer chromatography. SIMBAG-FEED Report 4.6. RIKILT, 2005). TLC plates were dried at 60 °C for 30 min in a petri dish and then covered to a thickness of 1.5 mm with agar medium containing the bacterial suspension. After incubation over night at 35 °C about 5 mL of 2,3,5-triphenyltetrazolium chloride solution (0.1 g in 100 mL water) was poured on the medium and re-incubated for some minutes. The inhibition zones were measured and their hRf values were compared with those of antibiotic standards. Detection was considered positive if the hRf value of the sample’s inhibition zone is ±5 % of the standard.

Phytochemistry 105, 68-78 (2014). HPTLC bioautography of antifungal compounds in the roots of Morinda tormentosa on silica gel with petroleum ether - ethyl acetate 1:1, detection at UV 254 and UV 366 nm. The plates were incubated overnight at 37 ºC, after solidification of 20 mL of C. albicans suspension distributed over the plate. Bioautograms were sprayed with an aqueous solution of methyl thiazolyl tetrazolium chloride (2.5 mg/mL), followed by incubation at 37 ºC for 6h. Clear inhibition zones were observed against a purple background.