J. Chinese Trad. Patent Med. (Zhongchengyao) 20 (2), 23-25 (1998). TLC on silica gel with chloroform - ethyl acetate - methanol 50:1:1. Detection by spraying with 0.5% phosphomolybdic acid in ethanol and heating at 110°C for 8-10 min. Quantitation of b-cholesterol by densitometry at 600 nm, and of total flavonoids by UV spectrophotometry. Precision for b-cholesterol = 2.94% (n=4). Recovery 98.64 ± 2.28% (n=4).

of Crop Sci., Univ. of Illinois at Urbana-Champaign, 240 Edward R. Madigan Lab., 1201 West Gregory Drive, Urbana, IL 61801, USA): Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proc. Natr. Acad. Sci. USA 94, 6036-6041 (1997). TLC of N-acylated derivatives of L-homoserine lactone (acyl-HSLs) on RP-18 with methanol - water 3:2. The spots were visualized by overlay of a bacterial reporter strain. Spots of preparative TLC were scraped off and submitted to Electrospray ionization mass spectrometry (ESIMS).

Chinese J. Modern App. Pharm. (Zhangguo Xiandai Yingyong Yaoxue Zazhi) 18 (15), 385-387 (2001). TLC on silica gel with ethyl acetate - iso-butanol - acetic acid - water 10:2:5:3. Detection by exposure to iodine vapor. Comparison of the finger prints of the hydrolysis solution obtained at different conditions.

J. Planar Chromatogr. 17, 244-249 (2004). TLC of thirty compounds (coumarin derivatives) including by-products on silica gel with ethyl acetate - hexane 2:3 or, for more polar synthesized compounds, with methanol - dichloromethane 7:93 in a saturated chamber. Detection under UV light at 254 nm, and by derivatization if necessary, e. g. by iodine reagent. Quantitative determination by densitometry at 292 - 363 nm. Direct screening of the reaction mixture on the plate for antioxidant activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) test radical.

J. of Chromatogr. B 879 (30), 3565-4573 (2011). Presentation of a novel “target constituent knock-out” strategy applied for preliminary screening of antibacterial constituents in Calculus bovis. The strategy contained the following steps: 1) the single constituents (A-F) in C. bovis samples were knocked out by TLC on silica gel with toluene - acetic acid - water 30:25:2, detection under UV 366 nm; 2) the knocked-out constituents were identified by UPLC–ELSD; 3) the antibacterial activities of the knocked-out constituents and C. bovis samples on Staphylococcus aureus were evaluated by microcalorimetry combined with principal component analysis; 4) the activities of the knocked-out constituents and the total extract of C. bovis, also the interaction properties between these single constituents and the total extract were elucidated. The strategy proved to be useful for screening active constituents and elucidating the multi-component interactions in C. bovis, and helpful in understanding the pharmacodynamic actions and the quality control of traditional Chinese medicines.

Anal. Chim. Acta 218, 217-229 (1989). Illustration of the use of phase-transition matrix in the direct MS analysis of TLC plates to increase both the absolute signal intensity and the duration of the analyte signal in comparison with analyses without solvent or with liquid matrixes such as glycerol. Preservation of the original resolution of TLC separation by extraction of sample into the matrix without diffusion. Use of spatially resolved images of individual ions in pattern recognition programs that establish the correlations between all of the ions in the mass spectrum of a particular sample spot.

J. Liquid Chromatogr. 7, 2711-2718 (1984). Examination of errors contributing to the total variance of automated streaking technique and comparison of these results with computer optimized and zig-zag scanning approaches. The error due to inhomogenous sample distribution is shown to be the most important.

J. Planar Chromatogr. 2, 71-75 (1989). A validation procedure for TLC scanners is presented including determination of signal to noise ratios and instrumental sensitivity. Using test plates, the overall reliability can be determined. Validation of individual parts of the software is done by comparison of different algorithms, for example smoothing algorithms. Validation of spotting devices is shown to be possible by analysis of variances from different types of scans in order to separate errors created by positioning and measurement.