Anal. Chem. 78, 5736-5743 (2006). Structural analysis of neutral glycosphingolipids by direct coupling of TLC to MALDI-QIT-TOF MS/MS. TLC of neutral glycosphingolipids on silica gel with chloroform – methanol – water 65:35:8. The underivatized glycosphingolipid spots were directly subjected to MALDI-QIT-TOF MS after addition of a matrix solution of 2,5-dihydrobenzoic acid in acetonitrile – water 1:1, which proved to be suitable for MS/MS analysis with high sensitivity. MS/MS and MS/MS/MS spectra reveal the ceramide and long-chain base structures, as well as the sugar sequences. Furthermore, derivatization with primuline (0,01 % in acetone – water, 4:1), a non-destructive fluorochrome for lipid detection, was used to locate glycosphingolipids on a TLC plate prior the MS analysis. The coupling of TLC-immunostaining of glycosphingolipids to MALDI-QIT-TOF MS/MS is shown to be a powerful method to identify both the antibody-specific sugar and the ceramide structures.

Anal. Chem., 58, 2708-2714 (1986). Description of sample transfer approach to TLC/FTIR in which several separated components are moved simultaneously from the TLC plate to an IR transparent substrate (Ge-Sb-Se glass) prior to measurement of its diffuse reflectance spectrum. Transfer with minimal sample loss, decomposition, or contamination compared with previous techniques. Sample transfer, TLC/FTIR

Merck KGaA (Ed.): Chromatographie - Chronologie einer Analysentechnik - Praxis, Status, Trends, GIT Verlag mbH, Darmstadt, 96-100, ISBN 3-928865-21-8 (1996). Correlation between instrumental thin-layer chromatography and GLP (Good Laboratory Practice). Each step in TLC, e.g. sample application, development, evaluation, pre- or post chromatographic derivatization, is fully automated. The advantages and possibilities of each automated step are discussed. Instrumental TLC shows good reproducibility and high flexibility. Documentation regarding GLP is demonstrated by reports. Automated validation of instruments is shown.

Sz. Nyiredy, A. Kakuk (eds.): Planar Chromatography 2000, Lillafüred, Hungary, 24-26 June 2000, Res. Inst. for Med. Plants, p. 57-65. Combined application of OPLC-DAR (overpressured layer chromatography - digital autoradiography) and HPLC-RD (high performance liquid chromatography - radioactive detector) solves complex problems appearing during in vivo metabolism study. OPLC-DAR is more sensitive than HPLC-RD and excellent for getting fast information about the existence of conjugates. In case of small amount of sample with low activity, OPLC-DAR is the only possibility for getting qualitative and quantitative information.

Instrument. 4, 24-30 (1984) (Chinese). (A software for the computerization of thin-layer chromatography). Detailed description of a software system for the computerization of TLC, including real-time data acquisition and processing, algorithms, result presentation, etc.

J. Planar Chromatogr. 10, 281-285 (1997). Introduction of novel software for the processing of qualitative data from two parallel TLC analyses simultaneously, based on the comparison of libraries of corrected RF values and in situ UV spectra. The essential part of the program is the combination report which lists the substances found on both systems. TLC of codeine, promazine, clomipramine, cocaine on silica with toluene - acetone - 94% ethanol - 25% NH3 45:45:7:3 and of hydroxyzine, lidocaine, codeine, and morphine on RP-18 silica with methanol - water - 37% hydrochloric acid 50:50:1. Densitometry by absorbance at 220 nm and measurement of spectra at 190-400 nm. Also published in Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 213-221 (1997).

J. Chromatogr. Sci. 54 (4), 639-646 (2016). Presentation of a low-cost method for digital image analysis of the separation results of colorless analytes on TLC plates. Quantitative TLC-digital image analysis was performed using a universal staining reagent (iodine vapor), an office scanner and a commonly available software (Microsoft Paint) for analysis of red, green and blue colors (RGB values). Urinary creatinine is used as a model analyte to represent a sample in complicated biological matrices. TLC on silica gel with butanol – ammonia solution – water 4:1:5 up to 6 cm. Detection by exposure to iodine vapors for 30–60 min. The Green value offered the best results in the linear working range of 0.08 - 0.93 mg/mL. The precision (%RSD) was 2.0 % (n=10), and the LOD 0.24 µg per zone. Comparison of the urinary creatinine concentrations determined by TLC-digital image analysis using the green value calibration graph with results obtained by HPLC showed that both are well consistent.

J. Chromatogr. 437, 221-229 (1988). TLC on silica with methanol. Detection under UV. Elution with methanol. Further separation by HPLC.