Microbiol. Res. 268, 127295 (2023). HPTLC of cardiolipin phospholipids in the deletion mutants of two cardiolipin synthetase genes, clsA (UM270 ΔclsA) and clsB (UM270 ΔclsB), in the rhizobacterium Pseudomonas fluorescens, on silica gel with chloroform - methanol - water 14:6:1 for the first dimension and chloroform - methanol - glacial acetic acid 13:5:2 for the second dimension. The lipid composition of UM270 wt, UM270 ΔclsA and UM270 ΔclsB mutant strains was determined by labeling with [1-¹⁴C] acetate. Detection by iodine staining and radioactive membrane lipids were visualized by exposure to autoradiography film or a Phosphor Imager screen. Individual lipids were quantified using an image software.

J. Strait Pharm. 22 (12), 59-60 (2010). TLC of the extracts of the title traditional Chinese medicine 1) for red Ginseng, on silica gel with benzene - ethyl acetate - formic acid 20:16:3, detection by spraying with 3 % FeCl3 in ethanol and viewing under daylight; 2) for Chinese Taxillus twig, on silica gel with water-saturated toluene - ethyl acetate - formic acid 5:4:1, detection by spraying with 5 % AlCl3 in ethanol; 3) for Hawthorn, on silica gel with toluene - ethyl acetate - glacial acetic acid 24:8:1, detection by spraying with 3-10 % sulfuric acid in ethanol and heating at 105 °C until the zones were detected; 4) for Alisma orientale, on silica gel with toluene - ethyl acetate - formic acid 14:7:2, detection by spraying with acetic acid - concentrated sulfuric acid - ethanol 1:1:1, heating at 105 °C until the zones were detected and viewing under daylight. Identification by comparison with the standards of the individual drug.

J. Agric. Food. Chem. 38, 1417-1422 (1990). TLC of 3-phenoxybenzaldehyde and 11 metabolites on silica with ethyl acetate - formic acid - water 35:2:2, hexane - toluene - acetic acid 3:15:2, toluene - ether - acetic acid 75:25:1. Detection by autoradiography. Quantification after methylation using HPLC.

3rd Communication: Identification of metabolites in rat urine. Arzneim.-Forsch./Drug Res. 46, 127-133 (1996). TLC of montirelin hydrate and 3 metabolites (deamidation and deprolination products) on silica with ethanol - 0.1 N hydrochloric acid 10:3 and chloroform - ethanol - 28% NH3. Detection by autoradiography, visualization of authentic compounds and nonradioactive metabolites under UV 240 nm.

J. Liq. Chromatogr. Relat. Tech. 38, 381-389 (2015). Review of the materials, techniques and instruments for the detection and quantification of radiolabeled compounds by planar radiochromatography. Special reference is made to sample preparation, stationary phases (including instant TLCD sheets), initial zone application, mobile phases, stationary phase development, and zone detection and quantification.

J. Agric. Food Chem. 39, 594-599 (1991). TLC of propargite and metabolites on silica with methanol - chloroform 3:7, detection by autoradiography. Quantification after spraying with water and elution of radioactive bands by LSC.

Anal. Biochem. 242, 152-155 (1996). HPTLC of phosphatidylinositol 3,4,5-trisphosphospate, phosphatidylinositol 3,4-bisphosphosphate and phosphatidylinositol 4,5-bisphosphosphate on silica gel impregnated with 5% boric acid solution in methanol, and of phospatidylinositol 4-phosphate and phosphatidylinositol 3-phosphate on NH2 impregnated with 5% boric acid solution in methanol. Development with 1-propyl acetate - 2-propanol - ethanol - 6% NH3 1:3:1:3. Visualization by immersion in 5% CuSO4 solution in 8% aqueous H3PO4 and heating at 180°C for 15 min. Quantitative determination by autoradiography and by densitometry at 366nm.

PLoS ONE 9(1), e1003109 (2013). In order to understand how Borrelia burgdorferi acquires cholesterol from its host, five experiments were performed. HPTLC on silica gel with chloroform – methanol 17:3, the lipids were extracted through the Bligh and Dyer method: (1) from the spirochete incubated 4 h in a medium containing 4 mg/L BODIPY-cholesterol (fluorescing only in hydrophobic environment), (2) from the spirochete incubated 2 h in a medium containing 10 µCi/L tritiated cholesterol, (3) from HeLa cells incubated with the spirochete itself charged with tritiated cholesterol (after removal of all spirochetes). The same analysis was applied (4) to the purified outer membrane vesicles of the spirochete previously charged with BODIPY-cholesterol. In cases (1) and (4), UV detection showed that the BODIPY-cholesterol is incorporated into the cholesterol glycolipids of B. burgdorferi, whereas without incubation it has the same hRf as cholesterol. In cases (1), (2) and (4), iodine staining made free cholesterol and cholesterol-glycolipids (galactopyranosides) visible, but no other (cholesterol-free) lipids. In case (2), the chromatogram was sprayed with EN3HANCE Spray (DuPont), exposed using BioMax MR Film (Kodak) for 4 and 14 days at −80 °C and developed using a Medical Film Processor Model SRX-101A (Konica); the radiolabelled cholesterol was shown incorporated into the cholesterol-glycolipids. In cases (2) and (3), the radiolabelled zones on the layer were scraped off and analyzed by liquid scintillation counting; cholesterol-glycolipids were found (more than free cholesterol) transferred from the spirochete to the HeLa plasmalemma.