Pharm. Res. 33, 328-336 (2016). HPTLC of a derivative of a novel D-peptide with improved binding to Aβ oligomers (3H-radioactively labelled RD2) on silica gel with 2-butanol - pyridine - ammonia (28 %) - water 39:34:10:26. 3H detection by placing the plates on phosphor imaging plates for autoradiography. The method was useful to determine the stability of 3H-labelled RD2 in mouse plasma.

J. Agric. Food Chem. 39, 995-999 (1991). TLC of dicamba and 2,6-DCSA (dichlorosalicylic acid) on silica against standards with toluene - acetone - acetic acid 75:20:5, ethyl acetate - acetic acid 20:1 or chloroform - ethanol - acetic acid 85:10:5. Visualization under UV or by autoradiography. Quantification after elution by LSC.

Arzneim.-Forsch./Drug Res. 48, 408-414 (1998). TLC of 153Sm-Ca/Na-EDTMP (ethylenediamine tetramethylene phosphonic acid) on cellulose with NH3 25% - methanol - water 1:10:20. Detection by autoradiography.

Planta Medica 82(3), 238-243 (2016). Pure 6-gingerol was submitted to sulfation either with purified human sulfatases (SULT1A1, SULT1A2, SULT1A3, SULT1B1, SULT1C2, SULT1C3, SULT1C4, SULT1E1, SULT2A1, SULT2B1a, SULT2B1b, SULT4A1, and SULT6B1) or with cytosol of human organs (lung, liver, small intestine, kidney), in presence of [35S]-marked 3’-phosphoadenosine 5’-phosphosulfate, and, after centrifugation (13000 rpm, 3 min), the supernatant was analyzed by TLC on silica gel with acetic acid – n-butanol 2:1. The plate was dried with a hair-drier. Detection by autoradiography on an X‑ray film; the radioactive band corresponding to the [35S]-sulfated 6-gingerol (hRf 76) was cut out and eluted with 0.5 mL water into a glass vial for liquid scintillation counting. The strongest 6-gingerol-sulfating activity was exhibited, among the enzymes tested, by SULT1A1 (followed by SULT1C4, SULT1A3, SULT1E1, SULT1A2 and SULT1B1, the other sulfatases being inactive), and, among the organ cytosols, by the small intestine (followed by liver, lung and kidney). The same procedure was applied to detect, after spin filtration, the same [35S]-sulfated 6-gingerol produced from 6-glycerol and [35S]-sulfate in presence of plate-cultured human cells HepG2 (hepatoma) and Caco-2 (colon adenocarcinoma); the amount of the produced sulfated form clearly depended on the gingerol concentration.

J. Agric. Food Chem. 39, 588-593 (1991). TLC of carbofuran and metabolites on silica with petrol ether - chloroform - ethanol 2:2:1. Detection after autoradiography by liquid scintillation.

J. Planar Chromatogr. 11, 25-29 (1998). Description of the powerful off-line combination of rapid, high-resolution overpressured-layer chromatography (OPLC) with the high sensitivity and rapidity of digital autoradiography (DAR), by which qualitative and quantitative analysis of radiolabeled metabolites in biological samples can be performed relatively quickly. OPLC/HPTLC of deramciclane (1R,2S,4R-(-)-N,N-dimethyl-2-[(1,7,7-trimethyl-2- phenylbicyclo[2,2,1]hept-2-yl)oxy]-ethaneamine 2-(E)-butanedioate) on silica gel with n-butanol - acetic acid - water 4:1:1, ethyl acetate - ethanol - triethylamine 25:25:2, acetonitrile - n-butanol - acetic acid - water 25:17:4:4 or 44:30:7:19. For comparison to the radioactivity signal, UV and fluorescence signals were evaluated at 254 and 366 nm.

J. Agric. Food Chem. 56, 8050-8057 (2008). HPTLC of [2-14C]-cymoxanil in the cultures (medium and mycelium) and in the cell-free extracts of Botrytis cinerea on silica gel with hexane - ethyl acetate - acetic acid 70:30:1. The most polar products were separated on RP-18 (impregnated with a methanolic solution of tetrabutylammonium bromide 70 mM and dried at 80 °C for 5 min) with phosphate buffer (0.01 M, pH 6) – methanol 11:9. Detection by autoradiography. The hRf value of cymoxanil was 35 on silica gel. Different metabolites were detected using both stationary phases.

J. Chromatogr. A 1520, 9-22 (2017). A review focused on application of TLC as simple, rapid, popular and inexpensive method for lipophilicity assessment. Discussion of the principles and methodology of quantitative structure retention relationship (QSRR) employed for lipophilicity prediction from retention data. Comparison of traditional and unconventional TLC systems applied for lipophilicity assessment. Overview of the applications of TLC retention constants and use of bioautography as a TLC method complementary in quantitative structure activity relationship (QSAR) studies. Discussion of the advantages and limitations of the method, and application of less common planar chromatography modes for drug discovery process.