Asian J. Chem. 20(7), 5409 - 5413 (2008). HPTLC of atomoxetine HCl on silica gel with acetonitrile - acetic acid 9:1. Quantitative determination by absorbance measurement at 269 nm. The method was linear in the range of 100-1000 µg/mL. The recovery was 99.8 %. The method was suitable for routine analysis of atomoxetine HCl in its pharmaceutical preparations.

62nd Indian Pharmaceutical Congress Abstract No. F-253 (2010). TLC of ambroxol hydrochloride and doxophylline on silica gel with n-butanol – toluene – ethyl acetate – 25 % ammonia 50:30:20:1. The hRf values were 36 and 45 for doxophylline and ambroxol, respectively. Quantitative determination by absorbance measurement at 258 nm. The method was linear in the range of 300-1100 ng/band for ambroxol and 100-1100 ng/band for doxophylline.

Food Control. 68, 220-228 (2016). HPTLC of biogenic amines (putrescine, histamine, tyramine and agmatine) in microbial cultures on silica gel with chloroform – benzene – triethylamine 12:9:2. Quantitative determination by absorbance measurement at 254 nm.

J. of Medicinal Chemistry 32, 297-301 (1989). TLC of N- (dipeptidyl)-S-acetylcysteamine and N, N’- (dipeptyl)cystamine on silica. Detection under UV, exposure to iodine vapor or spraying with ninhydrin.

Talanta 38, 1303-1307 (1991). Use of o-phthalaldehyde in sulfuric acid solution as spray reagent in TLC of some tryptophan derived indole alkylamines on silica with ethyl acetate - propan-3-ol - conc. NH3 45:35:20. Detection limits, from 20 to 100 ng per spot. Application examples.

Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 47-68 (1997). Measurement of retention data (expressed as the logarithm of capacity factors, log k') for twelve test alkanolamines indicating lysosomotropic activity in eighty planar chromatography systems employing eight different silica gel layers with varied chemically bonded phases. Aqueous binary mobile phases containing methanol or acetonitrile as organic modifier in five different proportions were applied in TLC experiments.

J. Planar Chromatogr. 14, 21-23 (2001). TLC of the DIPABTC derivatives of eight monoamines (adrenaline, b-phenylethylamine, histamine, noradrenaline, octopamine, serotonin, and tyramine) on silica gel in a horizontal chamber at 24°C with 15 mobile phases. Higher sensitivity was achieved when the colored derivatives were placed in HCl vapor resulting in deep red-brown spots. Simple and quick TLC method using a newly synthesized derivatization reagent.

Anal. Bioanal. Chem. 387, 1083-1093 (2007). A new HPTLC method for trace analysis (low µg/kg range) of the five heterocyclic aromatic amines PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine), MeIQx (2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline), 4,8-DiMeIQx (2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline), norharmane (9H-pyrido[3,4-b]indole) and harmane (1-methyl-9H-pyrido[3,4-b]indole) in meat samples has been established. HPTLC on LiChrospher silica gel WRF with methanol – chloroform 1:9. Using the ADC2, the plate activity was adjusted to 34 % relative air humidity with MgCl2 x 6H2O for 15 min and, at the same time, chamber saturation with 35 mL aqueous ammonia 28 % – ultrapure water 1:4 was performed for 20 min, followed by alkaline plate preconditioning for 15 min. The development was performed in a fresh chamber. Quantitative determination by absorbance measurement at UV 262 nm and 316 nm, and fluorescence measurement at UV 366/>400 nm. The UV wavelength 316 nm was later substituted by 313/>340 nm for a more selective and sensitive determination of PhIP in the meat matrix. Mass spectrometric analysis was performed in ESI+ mode for confirmation of positive findings. The method was validated according to ICH guidelines. Repeatability was better than 3.3 % (n=14), the intermediate precision (n=6, peak area) was 0.4 % (PhIP), 0.6 % (MeIQx), 0.7 % (4,8-DiMeIQx), 0.9 % (norharmane) and 1.1 % (harmane). Reproducibility of the migration distance was better than 1.3 % (n=6). LODs were 4 ng/band for PhIP, 5 ng/band for MeIQx, 4 ng/band for 4,8-DiMeIQx, and 0.4 ng/band each for norharmane and harmane. LOQs were 6 ng/band for PhIP, 14 ng/band for MeIQx, 11 ng/band for 4,8-DiMeIQx, and 0.8 ng/band each for norharmane and harmane. Selectivity in the meat matrix was proven. Confirmation of the substances found in meat was performed by MS. In the working range RSDs of the calibration functions were between 1.9 and 3.6 %.