Proc. Intern. Symp. on Planar Separations, Planar Chromatography 2001, pp. 355-362. TLC of lysine, threonine, homoserine, tryptophan, and phenylalanine on silica gel with 1-propanol - 25% NH3 11:9, 2-propanol - acetone - water - 25% NH3 25:25:7:6, 2-propanol - ethyl acetate - 25% NH3 - water 40:40:3:50 and 2-propanol - 25% NH3 7:3. Detection of tryptophan by immersion for 40 s in a 0.5% solution of 4-dimethylamino-benzaldehyde in ethanol containing 5% conc. sulfuric acid and heating at 110°C for 5-7 min. Amino acid spots were scanned with two different densitometers.

J. Planar Chromatogr. 18, 251-252 (2005). TLC of 22 silylated amino acids on silica gel with butanol. After spotting the plates with the amino acid solutions the samples were sprayed with hexamethyldisilazane reagent (1 % solution in acetone), dried, heated at 110 °C for 20 min, and cooled. The developed plates were dried, sprayed with ninhydrin (0.25 % solution in acetone), dried completely, then further heated at 110 °C for 20 min. Detection limits of this method within 10 min are comparable with those of other methods.

Pharm. Res. 33, 328-336 (2016). HPTLC of a derivative of a novel D-peptide with improved binding to Aβ oligomers (3H-radioactively labelled RD2) on silica gel with 2-butanol - pyridine - ammonia (28 %) - water 39:34:10:26. 3H detection by placing the plates on phosphor imaging plates for autoradiography. The method was useful to determine the stability of 3H-labelled RD2 in mouse plasma.

J. Chromatogr. 357, 139-146 (1986). TLC of insulin on cellulose with pyridine - butanol - amyl alcohol - MEK - formic acid - acetic acid - water 25:20:15:10:3:3:25. Detection by spraying with acid violet 6B and scanning at 530 nm.

The effect of histidine at the P2 subsite on the inhibition of aspartic proteinases. J. of Med. Chemistry 31, 625-629 (1988). TLC of two new inhibitors of the aspartic proteinase porcine pepsin on silica with 10% methanol in methylene chloride, methylene chloride - methanol - NH3 85:10:1 or 80:20:2, butanol - acetic acid - water 4:1:1, butanol - acetic acid - pyridine - water 15:3:12:10. Visualization under UV and with ninhydrin and 5% phosphomolybdic acid in ethanol.

J. of Medicinal Chemistry 32, 984-989 (1989). TLC of N8-acetylpermidine analogues on silica with methanol - chloroform - NH3 10:10:1. Visualization with ninhydrin spray.

Planta Med. 58, 263-265 (1992). TLC of plant extracts containing a peptide on silica with acetone – methanol – 10% NH3 3:1:1, butanol – methanol – 10% NH3 3:1:1 or chloroform – methanol 1:1. Also TLC on silica impregnated with 5% liquid paraffin in petrol ether with methanol – water 1:4. Detection by spraying with Dragendorff’s reagent, 5% FeCl3 or 2% ninhydrin in acetone.

J. Chromatogr. B 752 (2), 311-322 (2001). The peptide mix obtained from in-gel or on-blot degestion wax analysed directly after degestion or after concentration on POROS R2 beads. 2 DE of Mycobacterium bovis BCG Chicago cell proteins. Eight protein spots were identified using four preparation procedures for MALDI-MS. Overall, on-blot degestion was as effective as in-gel degestion. Whereas higher signal intensity resulted after concentration, hydrophilic peptides are better detected by direct measurement of the peptide mix without POROS R2 concentration.