J. Chromatogr. 537, 494-496 (1991). TLC of 20 amino cards on silica with butanol - acetic acid - water 40:5:7. Detection by spraying with a freshly prepared solution of formaldehyde (37%, 15 mL) + acetyl acetone (7.8 mL), diluted to 100 mL with acetate buffer (pH 4.7), and by heating at 100 °C for 10 min. Detection limits, 0.26 x 10-4 - 6.9 x 10-4 mmol. Comparison of the sensitivity of this reagent with ninhydrin reagent.

J. Planar Chromatogr. 6, 43-50 (1993). Separation of the 20 common protein PTH amino acid derivatives by AMD on silica. The wide range of polarity of the sample and the limited separation length dictated by the range of mobile phase velocities available prevented the identification of any single solvent gradient which could simultaneously provide adequate resolution of the PTH amino acid derivatives located at the front or end of the chromatogram. A more productive approach was to employ group separations of the polar and moderately polar PTH amino acid derivatives in 2 runs.

Identification of an extract of Althea officinalis and determination in a medical herb tea. Deutsche Apotheker Zeitung 135, 1147-1149 (1995). Sample preparation via cation exchange column to obtain several amino acids. TLC on silica with 1-butanol - acetic acid - water 75:19:19 including 0.25 % ninhydrin. Quantification of alanine by densitometry after heating at 496 nm. Recovery rate 99%.

Chromatogr. (Sepu) 14, 259-263 (1996). Two dimensional TLC of 30 amino acids, 40 organic acids and 20 nucleosides and related compounds on cellulose - silica with 1) isopropanol - ethyl acetate - acetone - methanol - para-amyl alcohol - NH3 - water 9:3:3:1:1:3:3, butanol - isopropanol - acetone - formic acid - water 18:8:8:3:6, 2) tert-butanol - ethanol - NH3 - water 5:6:1:3, isopentanol - petrol ether - ethanol - formic acid - propionic acid - water 45:100:25:20:1:1, 3) isopropanol - ethyl acetate - methanol - NH3 - water 5:3:1:3:1, isopropanol - saturated (NH4)2SO4 - water 4:77:19, for both direction, respectively. Detection under UV 254 nm, by spraying with 0.1 % acridine in ethanol, by spraying with ninhydrin-cadmium acetate reagent. Detection limit 10-10 - 10-11 mol, 2x10-8 - 5x10-8 mol, and 1x10-8 - 3x10-8 mol, respectively.

Chinese Anal. Chem. (Fenxi Huaxue) 26, 1047-1051 (1998). TLC of amino acids on silica gel with 1) chloroform - methanol - NH3 conc. 2:2:1, 2) phenol - water 3:1. Detection under UV 254 nm. Identification of tryptophan and histidine by FT-SERS. Detection limit 8 µg.

J. Sliwiok (ed.): Acta Chromatographica 10, 234-235 (2000). TLC on RP18W, silica gel/kieselguhr, and kieselguhr impregnated with paraffin oil with different mixtures of mobile phase. Best separation on RP18W with potassium hydrogen phosphate (0.1 M) - water - methanol 1:3:1. Postchromatographic derivatization with ninhydrin.

J. Planar Chromatogr. 17, 314-315 (2004). Separation of 22 amino acids on silica gel with n-propanol - water 7:3. Detection by spraying with 1) 5 % 4-hydroxyacetophenone in acetone, followed by drying in air until all solvent had completely evaporated, and heating in an oven at 110 °C for 10 min, and, after cooling, spraying with 2) 0.4 % isatin-5-sulfonic acid (sodium salt) in ethanol - water 4:1, followed by drying in air and heating for 10 min at 110 °C. Detection limits were betwen 0.1 and 2 µg.

Anal. Chem. 79, 486-493 (2007). TLC of methylene blue and methyl red on monolithic phase with ethyl acetate - ethanol -water 6:4:3 and 3:2:1 with chamber saturation for 30 min. After development the plates were dried and scanned with MALDI. TLC separation of fluorescamine labeled proteins (insulin, cytochrome c, lysozyme, and myoglobin) with 40 or 55 % aqueous acetonitrile and 0.1 % trifluoro acetic acid with chamber saturation for 30 min. Detection under UV 366 nm. Preparation of the monolithic layer: The polymerization mixture consisted of butyl methacrylate, ethylene dimethacrylate, 1-decanol, cyclohexanol, and 2,2-dimethoxy-2-phenyl-acetophenone.