Part III. J. Planar Chromatogr. 6, 282-288 (1993). Separation of selected hydroxy and amino acids into their enantiomers by TLC on a chiral stationary phase. Proposition of a new topological index which enables distinction between isomers of D and L configuration. Separation on Chir HPTLC plates (E. Merck) of D- and L-hydroxy acids (lactic, mandelic, hydroxyvaleric and hydroxycaproic acid) with acetonitrile - water 3:2, and of amino acids (methionine, valine, leucine, serine, and isoleucine) with methanol - water - acetonitrile 1:1:4. Detection of hydroxy acids by immersion into a solution (50 mg/100 mL) of thymolphthalein in ethanolic sodium hydroxide (0.1%) and of amino acids by immersion into 0.3% ninhydrin in acetone and heating at 110 °C for 10 min.

J. Chinese Herb Med. (Zhongcaoyao) 26, 592-593, 599 (1995). TLC of cholic acid and bilirubin on silica with ether - acetic acid - chloroform 2:1:2. Detection by spraying with 30% sulfuric acid and heating at 105 °C for 10 min. TLC on silica with butane - ethyl acetate - acetic acid - methanol 6:32:1:1. Detection by spraying with 15 % sulfuric acid in ethanol and heating. Quantification according to China Pharmacopoeia. Two-dimensional TLC of amino acids on silica with butanol - acetic acid -water 4:1:1, and phenol - water 3:1. Detection by spraying with 0.5% ninhydrin in ethanol.

Chromatographia 43, 301-303 (1996). TLC of a mixture of 13 DABS-amino acids on silica with eluents containing increasing concentrations of ethyl acetate in heptane and chloroform by multiple gradient development. Detection by densitometry at 485 nm.

J. Liq. Chrom. & Rel. Technol. 21, 427-432 (1998). HPTLC of 19 amino acids on cellulose with n-propanol - water 7:3 in a paper-lined chamber. After development and detection with ninhydrin reagent (0.3 g in 100 mL of n-butanol and 3 mL of acetic acid) densitometry at 610 nm.

J. Agric. Food Chem. 49, 1493-1501 (2001). TLC of 5-(4,5-dihydroxy-2-imino-1-imidazolidinyl)norvaline, 5-(2-imino-5-oxo-1-imidazolidinyl)norvaline, N5-[[carboxymethyl]amino](imino)methyl]ornithine on silica gel with n-butanol - water - acetic acid - pyridine 4:2:3:3. Visualization with ninhydrin.

J. Planar Chromatogr. 19, 401-404 (2006). TLC of methionine, L-cystine, calcium pantothenate, vitamin B1, vitamin B7, and p-aminobenzoic acid on silica gel in a pre-saturated chamber with n-propanol - water - chloroform 5:2:1. Quantification of methionine was based on the oxidation and reaction with leuco xylene cyanol FF solution, which formed a blue dye. Quantitative determination by absorbance measurement at 613 nm.

J. Chromatogr. A 1558, 77-84 (2018). Investigation of the influence of concentration of ion-pairing additive, as well as concentration and type of organic modifier of the mobile phase on migration of peptides in a pressurized planar electrochromatography (PPEC) system with octadecyl silica-based adsorbent. Comparison of the results with those obtained by similar HPTLC and PPEC systems. Discussion of the influence of particular variables on retention, electrophoretic mobility of solutes and electroosmotic flow of the mobile phase, of the co-influence of these factors on the final selectivity of peptide separation, and of the difficulty of independent optimization of these factors due to their impact simultaneously on the retention, the electrophoretic mobility, and the electroosmotic flow. The pH of the mobile phase was the main variable for optimization of the PPEC system. Extensive tailing of peptide zones in the PPEC was observed in comparison to similar HPTLC systems.

Part VIII. Methylamides and dimethylamides of D-Leu5(-enkephalin) and D-Ala, D-Leu5(-enkephalin). Pol. J. Pharmacol. Pharm. 38, 391-402 (1986). TLC of enkephalin analogs on silica with chloroform -methanol - acetic acid 25:2:1, hexane - ethyl ether - acetic acid 11:7:1, chloroform -methanol -17 % NH3 15:1:0.1, chloroform - methanol - acetic acid 19:2:1, chloroform -methanol acetic acid 19:1:1, chloroform - methanol -25 % NH3 10:1:0.1, butanol - acetic acid - water -4:1:5 upper layer. Visualization by exposure to iodine vapor or spraying with 0.2 % ninhydrin in ethanol or with cerium-molybdenum reagent.