Chromatogr. Sci. 55, 71-85 (1991). A review with 50 references. Discussion of general optimization techniques that can be used in the different steps of the TLC process. Method for the choice of solvent in the chromatographic separation step are given.

Chromatographia 35, 149-152 (1993). Description of a computer-assisted mixture design simplex method for the optimization of the solvent system. Separation of a mixture of 5 benzoic acid derivatives in reverse-phase and of 12 PTH-amino acids in normal phase. Method demonstrated using a two-factor selectivity rectangle concept and a special polynomial regression of 9 TLC runs, with the Rf difference as the selection criterion and with connection to a general simplex method.

Dünnschicht-Chromatographie. InCom Sonderband 1996, 124-138. Comparison of six different optimization methods based on the relationship of coupling the retention parameters with the binary mobile phase composition, as proposed by Snyder, Schoenmakers, Lu Peichang and Lu Xaoming, Purnell, Kowalska, and Horvath and Melander.

J. Planar Chromatogr. 12, 365-372 (1999). Development of a computer program for optimization of mobile phase composition in TLC and examination of this method for chromatography of cyanobacterial hepatotoxins on normal-phase TLC plates and for phenolic compounds on RP layers. HPTLC of microcystins (cyclic heptapeptides) on silica gel with combinations of n-propanol - ethyl acetate - water. Densitometry at 240 nm. TLC of phenolic acids (catechin, ellagic acid, gallic acid, kaempferol, methyl gallate, rutin) on RP-18. Videodensitometry at 254 nm.

J. Planar Chromatogr. 28, 241-247 (2015). TLC of (1) citicoline sodium in the presence of its (2) alkaline degradation products in pharmaceutical oral solution on silica gel with ammonia - ethyl acetate - triethylamine 12:7:1. Quantitative determination by absorbance measurement at 272 nm. The hRF value was 35 for (1) and 10 for (2). Linearity for (1) was between 0.6 and 2.2 µg/zone. The intraday precision was 0.2 % and interday precision was 1.2 % for (1). The LOD and LOD of (1) were 41 ng/zone and 237 ng/zone, respectively.

J. Planar Chromatogr. 10, 290-297 (1997). TLC of miltefosine on silica with chloroform - ethanol - p-toluenesulfonic acid (0.25 mol/L) in NH3 25% 7:4:1. Detection by immersion into a copper(II) sulfate solution (10 g copper sulfate were filled up to 100 mL with 8% phosphoric acid) for a few seconds and heated at 180°C for 20 min. Quantification by densitometry at 500 nm. For detection of 1-octadecanol development in dichloromethane - methanol - 25% NH3 (100:1:0.2), followed by immersion into copper sulfate solution. Validation, specificity, linearity, accuracy, detection and quantification limit, precision, robustness, log P determination, method development, experimental details, and results are described in detail. Also published in Proc. 9th Internat. Symp. Instr. Chromatogr., Interlaken, April 9.-11., 289-303 (1997).

Acta Chromatographica 21(2), 251-258 (2009). Study of the relative lipophilicity, RM0, and specific hydrophobic surface area of eleven 3-hydroxy-1,2-benzoisoxazoles substituted in the benzene ring (two isomeric fluoro, three isomeric chloro, three isomeric bromo and dibromo derivatives, and a nitro derivative) by TLC on RP-18 with methanol – water mixtures. Comparison of lipophilicity RM0 with computed partition coefficients IAlogP, A logPs , clogP, milogP, logPKOWIN , and xlogP, and the best correlation (r> 0.9) was found between RM0 and logPKOWIN and xlogP values. Comparison of RM0 values with computed partition coefficients by principal-components analysis and comparison of the chromatographic behavior of 3-hydroxy-1,2-benzisoxazoles with that of their bioisosteric analogues 1,2-benzisothiazolonoles. It was found that the experimental RM0 values for both groups of compounds were in accordance with the equation RM0 = aRM0 + b (r>0.9).

Chromatographia 17, 221-227 (1983). Adsorption isotherms of polar solvents from solutions in non-polar diluents can be determined in a simple manner using a sandwich chamber for TLC. The experimental results for silica gel as the adsorbent and solvents of class B and AB indicate satisfactory agreement with results found by static and column break-through methods.