Biochemical Systematics and Ecology 24, 427-434 (1996). Preparative TLC of trans- and cis-isomers of 3-hydroxyprolinebetaine on silica with methanol - acetone - hydrochloric acid conc. 45:2:2.

(Oxidation of nicotine and chelating agent by mercury(II)-compounds). Pharmazie 62, 7-13 (2008). TLC of nicotine on silica gel with chloroform - ethanol - ammonia 80:20:1. Detection by spraying with Dragendorff reagent. TLC of iminodiacetic acid on silica gel with n-propanol - water 7:2 or with n-butanol - acetic acid - water 4:1:1. Detection by spraying with ninhydrin reagent.

Isolation of mycosporine hydroxyglutamicol from Nephroma laevigatum Ach. Phytochemistry 72, 1348-1357 (2011). HPTLC of mycosporines and mycosporines-like amino acids on silica gel with chloroform – methanol – water 6:4:1. The plate was dried in a stream of nitrogen and protected from light. Detection by absorbance measurement at the respective maximum wavelength between 310 and 360 nm. The hRfvalues of the compounds ranged between 20 and 80. HPTLC allowed a rapid and simultaneous comparison of 12-20 extracts for UV-absorbing compounds within 2 h.

J. Planar Chromatogr. 28, 144-151 (2015). TLC of L-cysteine on cellulose phase with 2-butanol - pyridine - glacial acetic acid - water 5:10:3:12. Just before development an equimolar amount of (1) manganese(II) acetate or (2) zinc(II) nitrate was added to the sample solutions. Detection in reflectance mode at 485 nm, concentration of L-cysteine and DL-cysteine in 70 % aqueous acetonitrile was 0.7 mg/mL. Developing distance was 7 cm, development time ca. 3 h. Derivatization by spraying with 0.5 % ninhydrin solution in 2-propanol, followed by heating for 5 min at 110 °C. The experiments demonstrate the spontaneous chiral conversion and spontaneous peptidization of L-cysteine, and the enantioseparation of L-cys and D-cys enantiomers is facilitated by chelating the transition metal cation (e.g. Mn(II)) with the cys ligands.

Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 56-57 (1984). Two dimensional TLC of PTH-amino acids on polyamide TLC membranes, impregnated with fluoresceine layers, having high porosity (65-80 % ), optimum pore diameter 0.l-0.2 micrometer. Development with 1) toluene-pentane- acetic acid 9:3:2, 2) 3.5 % acetic acid. Detection by UV

Nanjing Univ. (Natu. Sci). (Nanjing Daxue Xuebao, Ziran Kexue) 3, 524-526 (1984). (Chinese). (Separation and identification of amino acids in protein hydrolysates by thin-layer chromatography.) 2-dimensional TLC of protein hydrolysates on microcrystalline cellulose with butanol -2-butanone - NH3 - water 5:3:1:1 and isopropanol - formic acid 20:1:5. Detection by spraying with ninhydrin reagent.

Chromatographia 23, 207-208 (1967). TLC of 15 amino acids on silica, or on silica impregnated with halide ions, with n-butanol - acetic acid - chloroform 3:1:1. Detection by spraying with 0.2 % ninhydrin in acetone. Discussion of the effect of .the hydrophobic interactions between silica gel and amino acid molecules, of polarity and of the flow of the mobile phase on resolution. The method provided resolution of 10-11 amino acids from a 15 component mixture.

Anal. Chim. Acta 192, 309-313 (1987). Description of 3 fluorescent derivatization reagents for compounds having hydroxyl and/or amino groups, which were stable at room temperature and condense stechiometrically with alcohols, amines and amino acids in the presence of alkali to give strongly fluorescent derivatives. Pre-chromatographic derivatization followed by TLC and HPLC.