Anal. Bioanal. Chem. 387, 1083-1093 (2007). A new HPTLC method for trace analysis (low µg/kg range) of the five heterocyclic aromatic amines PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine), MeIQx (2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline), 4,8-DiMeIQx (2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline), norharmane (9H-pyrido[3,4-b]indole) and harmane (1-methyl-9H-pyrido[3,4-b]indole) in meat samples has been established. HPTLC on LiChrospher silica gel WRF with methanol – chloroform 1:9. Using the ADC2, the plate activity was adjusted to 34 % relative air humidity with MgCl2 x 6H2O for 15 min and, at the same time, chamber saturation with 35 mL aqueous ammonia 28 % – ultrapure water 1:4 was performed for 20 min, followed by alkaline plate preconditioning for 15 min. The development was performed in a fresh chamber. Quantitative determination by absorbance measurement at UV 262 nm and 316 nm, and fluorescence measurement at UV 366/>400 nm. The UV wavelength 316 nm was later substituted by 313/>340 nm for a more selective and sensitive determination of PhIP in the meat matrix. Mass spectrometric analysis was performed in ESI+ mode for confirmation of positive findings. The method was validated according to ICH guidelines. Repeatability was better than 3.3 % (n=14), the intermediate precision (n=6, peak area) was 0.4 % (PhIP), 0.6 % (MeIQx), 0.7 % (4,8-DiMeIQx), 0.9 % (norharmane) and 1.1 % (harmane). Reproducibility of the migration distance was better than 1.3 % (n=6). LODs were 4 ng/band for PhIP, 5 ng/band for MeIQx, 4 ng/band for 4,8-DiMeIQx, and 0.4 ng/band each for norharmane and harmane. LOQs were 6 ng/band for PhIP, 14 ng/band for MeIQx, 11 ng/band for 4,8-DiMeIQx, and 0.8 ng/band each for norharmane and harmane. Selectivity in the meat matrix was proven. Confirmation of the substances found in meat was performed by MS. In the working range RSDs of the calibration functions were between 1.9 and 3.6 %.

J. AOAC Int. 82, 17-24 (1999). Review of methodology for separation, detection, and quantitative determination of catecholamines, 5-hydroxytryptamine, and their acidic metabolites in biological fluids by TLC. Selected procedures, including fluorometric scanning densitometry for catecholamine acetyl derivatives and color scanning densitometry for acids, are described. - Structure and metabolism of catecholamines, general characteristics of analysis, TLC procedures (extraction, analysis of nonmodified CAs, analysis of derivatized CAs, application to body fluids), TLC of metabolites (extraction, separation, application to body fluids), selected method for amine determination (principle, materials, extraction, derivatization, TLC and quantitation) and selected method for acidic metabolite determination (principle, materials, methods). TLC as a simple, inexpensive method for the clinical laboratory.

62nd Indian Pharmaceutical Congress Abstract No. F-243 (2010). TLC of lidocaine hydrochloride and clotrimazole on silica gel with toluene – ethyl acetate – methanol – glacial acetic acid 45:30:20:1. Quantitative determination by absorbance measurement at 235 nm. The hRf value was 28 for lidocaine HCl and 70 for clotrimazole. Linearity was in the range of 200-1200 ng/band for lidocaine HCl and 100-600 ng/band for clotrimazole. Recovery was found to be 99.6 % for lidocaine HCL and 99.0 % for clotrimazole.

J. Ethnopharmacol. 175, 324-334 (2015). HPTLC of betaine in Achyranthes aspera root extract on silica gel with methanol - water 9:1. Detection by spraying with Dragendorff's reagent followed by 10 % ethanolic sulfuric acid and drying at 110 °C for 5 min. Quantitative determination by absorbance measurement at 520 nm. The hRF value for betaine was 36. Linearity was in the range of 1-5 μg/zone. LOD and LOQ were 0.10 and 0.13 μg/zone. The intermediate precision was below 2 % (n=3).

J. Planar Chromatogr. 2,.151-152 (1989). OPLC of capsaicin on HPTLC silica, prewashed in methanol and activated at 110°C, with toluene - acetone - chloroform 40:35:25. Visualization with 2,6-dichloroquinone-4-chloroimide (Gibbs’ reagent). A selective, rapid and simple method with good options for quantitative evaluation. Quantification by photometry.

(German). Parfümerie u. Kosmetik 77, 244-248 (1996). Semiquantitative TLC of free amidoamines on silica with chloroform - methanol - NHß 15:25:1. Detection by spraying with bromophenolblue solution.

obshchej. chim. 52, 2322- 2328 (1982). (Russian). (Investigation on the polarographic behavior of phtalimidodiazoketones.) TLC on silica with chloroform - acetone 9:1. Detection by spraying with 0.02 % diphenylcarbazone in chloroform and mercury sulfate.

J. Chromatogr. 294, 513-516 (1984). Detection on silica by spraying with borate solution and/or heating at various temperatures. Detection limits for epinephrine, dopamine and norepinephrine 2,2 and 4 ng, for metabolites about ten times higher.