PLoS ONE 9(1), e1003109 (2013). In order to understand how Borrelia burgdorferi acquires cholesterol from its host, five experiments were performed. HPTLC on silica gel with chloroform – methanol 17:3, the lipids were extracted through the Bligh and Dyer method: (1) from the spirochete incubated 4 h in a medium containing 4 mg/L BODIPY-cholesterol (fluorescing only in hydrophobic environment), (2) from the spirochete incubated 2 h in a medium containing 10 µCi/L tritiated cholesterol, (3) from HeLa cells incubated with the spirochete itself charged with tritiated cholesterol (after removal of all spirochetes). The same analysis was applied (4) to the purified outer membrane vesicles of the spirochete previously charged with BODIPY-cholesterol. In cases (1) and (4), UV detection showed that the BODIPY-cholesterol is incorporated into the cholesterol glycolipids of B. burgdorferi, whereas without incubation it has the same hRf as cholesterol. In cases (1), (2) and (4), iodine staining made free cholesterol and cholesterol-glycolipids (galactopyranosides) visible, but no other (cholesterol-free) lipids. In case (2), the chromatogram was sprayed with EN3HANCE Spray (DuPont), exposed using BioMax MR Film (Kodak) for 4 and 14 days at −80 °C and developed using a Medical Film Processor Model SRX-101A (Konica); the radiolabelled cholesterol was shown incorporated into the cholesterol-glycolipids. In cases (2) and (3), the radiolabelled zones on the layer were scraped off and analyzed by liquid scintillation counting; cholesterol-glycolipids were found (more than free cholesterol) transferred from the spirochete to the HeLa plasmalemma.

Carcinogenesis 15, 2829-2834 (1994). Separation of the title compounds, postlabelled with [g-32P] ATP, by TLC and HPLC with on-line detection of 32P.

Pharm. Res. 33, 328-336 (2016). HPTLC of a derivative of a novel D-peptide with improved binding to Aβ oligomers (3H-radioactively labelled RD2) on silica gel with 2-butanol - pyridine - ammonia (28 %) - water 39:34:10:26. 3H detection by placing the plates on phosphor imaging plates for autoradiography. The method was useful to determine the stability of 3H-labelled RD2 in mouse plasma.

J. Chromatogr. 668, 21-29 (1995). TLC of 73As-radiolabelled title compounds in aqueous solution, rat liver cytosol and urine of mice on cellulose with 1) methanol - NH3 8:2, 2) isopropanol - acetic acid - water 20:2:5. Detection by autoradiography. Oxidation of samples by hydrogen peroxide improved the separation and quantification of monomethylarsonate in both biological matrices.

Food Chem. 177, 441-446 (2015). HPTLC of radiolabelled bovine casein-derived peptide Met-Lys-Pro (14C-MKP) and its fragments Met-Lys (MK), Lys-Pro (KP) and Lys (K) in plasma on silica gel with acetonitrile - acetic acid - water 2:1:1. Quantitative determination of the amount of intact 14C-MKP in plasma by radioactivity measurement using Bio-Imaging. The hRF values of MKP, MK, KP and K were 50, 52, 34 and 28, respectively.

J. Agric. Food Chem. 46, 707-717 (1998). Purification and sample preparation TLC of diphenylamine, 3-hydroxy- and 4-hydroxy-diphenylamine, 4,4'-dihydroxy-diphenylamine on silica gel with n-hexane - ethyl acetate - NH3 10:10:1; 2 x, n-hexane - acetone -acetic acid 10:10:1, ethyl acetate - acetic acid - water 92:8:4, 1-butanol - acetic acid - water 3:2:2. Visualization by exposure to short wave UV light; radiochromatograms were obtained by scanning of the TLC plates. Application of two-dimensional TLC for the characterization of aglycons.

Biochem. Biophys. Res. Commun. 487, 702-708 (2017). HPTLC of the reaction products of different 14C-labeled substrates of the mevalonate pathway (MVA, MVA-5-P, MVA-3-P, MVA-5-PP) and the recombinant Flavobacterium johnsoniae protein on silica gel with n-propanol – 28 % ammonia – water 6:3:1. The distribution of radioactivity on the TLC plate was detected using a suitable image analyzer.

J. Lipid. Res. 38, 1702-1706 (1997). Two-dimensional TLC of 1,2- and 1,3-diacylglycerols, and of ceramides containing a-hydroxy and normal fatty acids, on silica gel in the first direction with 1) chloroform - methanol 10:1. In the second direction development with 2) hexane - ethyl ether - acetic acid 80:20:1, and after drying once more with 3) heptane - diisopropyl ether - acetic acid 15:10:1. Visualization with Dittmer and Lester reagent. For liquid scintillation counting the radioactive spots were visualized by exposure to iodine vapor, and scraped into counting vials.