Anal Chim Acta 1180 (2021) 338644. Review with 120 references on HPTLC combined with effect-directed assays (EDA) and high-resolution mass spectrometry (HRMS). Brief introduction of status quo of these emerging technologies, involving targeted, untargeted and predictive strategies. Hyphenated HPTLC has great potential for solving pressing analytical questions because it provides information on the effects from individual compounds in complex or natural samples, it allows separation in parallel, and the separation is combined with effect-directed detection using enzymatic or biological assays, capable to select the important compounds in a complex sample for further characterization by HRMS. Overview of emerging hyphenated technologies driven by HPTLC, including non-target high-throughput 8D hyphenation, minimum requirements for sample preparation and comprehensive effect evaluation, agonistic and antagonistic fast effect detection, identifying multi-potent or multi-modulating compounds and simulated on-surface digestion or metabolization for study of biotransformation. HPTLC is a versatile, creative and flexible open-format technique with the benefits of super-hyphenation, minimum requirements for sample preparation, detection of multi-modulating compounds or agonistic versus antagonistic effects, and miniaturization of on-surface metabolization. Discussion of the potential offered by the miniaturized open-source LabToGo system.

J. Planar Chromatogr. 34, 369-375 (2021). Review of the application of TLC and HPTLC for the analysis of inorganic anions between 1995 and 2020, including separation techniques in multicomponent mixtures.

Rapid Commun. Mass Spectrom. 35, 9013 (2021). Review of the application of direct analysis in real time (DART) combined with mass spectrometry (MS) detection in food science and industry published in the period from 2005 to this date. The applications described the use of HPTLC in preparation methods as well as the combination of HPTLC fingerprints and DART-MS with multivariate data analysis for the differentiation of natural propolis products.

J. Ethnopharmacol. 276, 114144 (2021). Review of analytical methods for the analysis of the main secondary metabolites detected and isolated from Ammi majus. The paper described TLC and HPTLC methods for the determination of coumarins in fruits.

J. Ethnopharmacol. 281, 114503 (2021). Review of the reported phytochemical studies of Sesamum indicum, including TLC and HPTLC methods for the analysis of major compounds. 

J. Ethnopharmacol. 275, 114054 (2021). Review of methods and their conditions for quality control of herbal formulations derived from Andrographis paniculata by analytical techniques, including TLC and HPTLC. The document provided specific validation data for the analysis of andrographolide in different systems.

Phytochem. Anal. 3091 (2021). Comprehensive review of the application of chromatographic methods for the quality control of fenugreek seeds. The document described systems for the analysis of fenugreek, including the determination of trigonelline, 4-hydroxyisoleucine and diosgenin in seeds from different origins and in herbal formulations.

Food Control. 127, 108113 (2021). Review of various techniques used for adulterant authentication in spices and herbs, including identification of unusual profile of a component, differentiation from the normal standard compound and identification of marker or foreign material in the product. The document provided an overview of chromatographic methods, including TLC and HPTLC for adulterant detection, and its combination with mass spectrometry.