Ind. J. Pharma. Sci. 71(1), 95-97 (2009). HPTLC of emtricitabine and tenofovir on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 265 nm. The calibration curve was linear between 200 and 1000 ng with a regression coefficient of 0.9995.

Abstract No. F-284, 61st IPC (2009). HPTLC of diclofenac sodium and thiocolchicoside on silica gel with toluene - ethyl acetate - methanol 5:3:2. The hRf value was 17 and 53 for thiocolchicoside and diclofenac sodium, respectively. Quantitative determination by absorbance measurement at 285 nm. The method was linear in the range of 50-300 ng/band for both drugs. Recovery was 100.5-101.1 %.

60th Indian Pharmaceutical Congress PA-213 (2008). HPTLC of atorvastatin calcium and amlodipine besilate on silica gel with chloroform - toluene - methanol - water 55:10:20:2. Quantitative determination by absorbance measurement at 242 nm. The calibration curve was found to be linear between 400 and 1200 ng/spot for both atorvastatin calcium and amlodipine besilate. The limit of detection and the limit of quantification for atorvastatin calcium were 100 and 400 ng/spot, and for amlodipine besilate 200 and 400 ng/spot, respectively.

Abstract No. F-261, 61st IPC (2009). HPTLC of quetiapine on silica gel with methanol - toluene 4:3. The hRf value was 41. Quantitative determination by absorbance measurement at 235 nm. The method was linear in the range of 100-500 ng/band. Recovery was 98.9 %.

Abstract No. F-270, 61st IPC (2009). HPTLC of fenoverine on silica gel with methanol - butyl acetate 1:4. The hRf value of fenoverine was 67. The linearity was in the range of 50 to 500 ng/band with a correlation coefficient of 0.9976. The limit of detection and quantification was 30 ng/band and 100 ng/band, respectively. The recovery was 100.1 %.

J. Planar Chromatogr. 23, 123-128 (2010). HPTLC of rifabutin and isoniazid on silica gel with dichloromethane - acetone - methanol 20:7:2 in a twin-trough chamber previously saturated for 25 min. Quantitative determination by absorbance measurement at 504 nm for rifabutin (hRf 84) and at 262 nm for isoniazid (hRf 48). The linearity range was 10-70 and 5-35 µg/mL, the correlation coefficient was 0.9991 and 0.9989, the precision (RSD, n = 6) 0.90 % and 0.71 %, and precision on different days (RSD, n = 3) was 0.89 % and 1.01 %. LOD was 180 µg and 90 µg/zone, LOQ was 540 and 270 µg/zone, and the system suitability (RSD, n = 6) was 1.41 % and 1.86 % for rifabutin and isoniazid, respectively.

J. Planar Chromatogr. 23, 141-147 (2010). HPTLC of p-aminobenzoic acid on silica gel (prewashed with the mobile phase) with diethyl ether - cyclohexane 5:1 in an unsaturated chamber. Quantitative determination by absorbance measurement at 270 nm. Detection by automatic spraying the following reagent solutions: 0.1 mol/L sodium nitrite solution, 0.1 mol/L hydrochloric acid, 0.03 mol/L ethanolic 8-hydroxyquinoline solution, and 10% sodium hydroxide solution. For each solution 2 spraying cycles were applied. After drying quantitative determination by densitometric scanning at 500 nm. Measured before derivatization at 270 nm calibration of p-aminobenzoic acid was perfomed between 50-1600 ng per spot. LOD was 170 ng/spot (calculated) and 50 ng/spot (estimated visually). LOQ (calculated) was 510 ng/spot. The correlation coefficient r was 0.9936 (peak area) and 0.9866 (peak height); the linear range was 200-1800 ng/spot. Measured at 500 nm after derivatization, LOD was 290 ng/spot (calculated) and 50 ng/spot (estimated visually). LOQ (calculated) was 870 ng/spot and 200 ng per spot (estimated visually). The correlation coefficient r was 0.9815 and 0.9538.

62nd Indian Pharmaceutical Congress Abstract No. F-251 (2010). TLC of levocetirizine and phenylpropanolamine on silica gel with methanol – ethyl acetate – toluene – ammonia 15:4:5:2. Quantitative determination by absorbance measurement at 210 nm. The hRf value was 30 and 60 for levocetrizine and phenylpropanolamine, respectively. The linearity was in the range of 45-270 ng/band for both drugs.