Abstract No. F-236, 61st IPC (2009). HPTLC of pantoprazole and mosapride citrate on silica gel (pre-washed with methanol) with ethyl acetate - benzene - methanol - 25 % ammonia 48:35:15:2 at room temperature. Quantitative determination by absorbance measurement at 250 nm or 276 nm. The method was linear in the range of 3-7 µg/band for both drugs. Recovery was between 99.0 and 103.5 %.

J. Liq. Chromatogr. Relat. Technol. 33, 778-785 (2010). TLC of ofloxacin and pefloxacin on silica gel with acetonitrile - formic acid - water 40:3:7 in chamber saturated for 30 min. Quantitative determination by densitometry at 295 nm for ofloxacin and at 280 nm for pefloxacin. The regression equations were achieved using computer program STATISTICA 7.1.

Phytochem. Anal. 21, 219-223 (2010). HPTLC of safranal in saffron extract and in a safranal-loaded nanoparticle formulation on silica gel with n-hexane - ethyl acetate 9:1. Quantitative determination by absorbance measurement at 310 nm. The hRf of safranal was 51. Linearity was between 0.5 and 5.0 µg/zone. The intra-day and inter-day precisions were 1.08-2.17 and 1.86-3.47 %, respectively. LOD was 50 ng/zone while LOQ was 150 ng/zone. The average recovery was 99.9 %. The proposed method provides significant advantages in terms of greater specificity and rapid analysis.

Eur. J. Med. Chem. 45, 3719-3725 (2010). HPTLC of sulpiride and mebeverine on silica gel with ethanol - methylene chloride - triethyl amine 35:15:1. Quantitative determination by absorbance measurement at 221 nm. The hRF of sulpiride and mebeverine was 42 and 62, respectively. Linearity was between 0.4-1.4 µg/band for sulpiride and 0.2-1.6 µg/band for mebeverine. Detection and quantification limits were 0.02 and 0.3 µg/band for sulpiride and 0.04 and 0.2 µg/band for mebeverine. The intra-day and inter-day precisions had a %RSD lower than 2.6 %.Recoveries (by standard addition) were 99.3 % and 100.7 % for sulpiride and mebeverine, respectively. The proposed method showed comparable statistical results with the standard HPLC method

J. Planar Chromatogr. 24, 99-104 (2011). TLC of candesartan and losartan on silica gel with 1,4-dioxane - hexane - 99 % formic acid 50:50:1. Quantitative determination by densitometry at 258 nm for candesartan and at 243 nm for losartan, videoscanning at 254 nm for both drugs. The hRf value for candesartan was 47 and for losartan 35. Linearity was between 0.2 and 1.4 µg/band for both drugs with correlation coefficients of 0.9997 and 0.9981 for candesartan, and 0.9986 and 0.9982 for losartan, for densitometry and videoscanning, respectively. Robustness (%RSD, peak area) was less than 1.9 and 0.8 % for candesartan, and 2.2 and 0.9 % for losartan in densitometry and videoscanning, respectively. The repeatability and intermediate precision (%RSD, two lowest amounts) were less than 3.6 and 4.7 % for candesartan and less than 4.7 and 5.3 % for losartan. Mean recoveries for candesartan were 103.8-104.9 % for densitometry and 99.2-100.7 % for videoscanning; for losartan the respective values were 100.8-105.4 % and 98.6-99.2 %.

J. Chil. Chem. Soc. 56, 702-705 (2011). HPTLC of lamivudine (1) and tenofovir disoproxil fumarate (2) in bulk drug and pharmaceutical dosage form on silica gel with chloroform - methanol - toluene 4:1:1. Quantitative determination by absorbance measurement at 265 nm. The hRf values of (1) and (2) were 27 and 51, respectively. Linearity was between 60-210 ng/zone for both. LOD and LOQ were found to be 20 and 40 ng/zone for (1) and 30 and 60 ng for (2). The intermediate/interday/intra-day precision was 0.6 % (n=6). Recovery (by standard addition) for (1) and (2) was between 98-102 %. The HPTLC method is suitable for routine analysis of lamivudine and tenofovir in pharmaceutical dosage form.

J. Planar Chromatogr. 24, 344-347 (2011). HPTLC of ivermectin (IVM) and albendazole (ALB) on silica gel with toluene - ethyl acetate - glacial acetic acid 12:8:1 in a twin-trough chamber saturated for 30 min. Quantitative determination by densitometry in absorbance mode at 247 nm. Linearity was between 0.12 and 0.54 µg/band for IVM and 8 and 36 µg/band for ALB. The recovery was between 98-101 % for IVM and ALB. The hRf value was 39 for IVM and 62 for ALB. LOD and LOQ were 0.02 and 0.09 µg/band for IVM and 0.08 and 0.1 µg/band for ALB. The intra-day and inter-day precision (n = 6) was 0.6 % and 1.1 % for IVM and 0.6 % and 1.2 % for ALB, respectively. Recovery (by standard addition) ranged from 98-101 % for both compounds.

J. Chil. Chem. Soc. 57, 1033-1035 (2012). HPTLC of propanolol hydrochloride (1) and flunarizine dihydrochloride (2) in combined dosage form on silica gel with methanol - toluene - ammonia 14:6:1. Quantitative determination by absorbance measurement at 267 nm. The hRf of compounds (1) and (2) were 63 and 48, respectively. Linearity was in the range of 800-4800 ng/zone for (1) and 200-1200 ng/zone for (2). Limits of detection and quantification were 25 and 75 ng/zone for (1) and 16 and 48 ng/zone for (2), respectively. Intermediate/intra-day/inter-day precision was below 2.0 % (n=6). Recovery for both (1) and (2) was between 99.3 and 100.9 %.