CAMAG® HPTLC Software visionCATS

    The visionCATS software organizes the workflow of the HPTLC analysis, controls the CAMAG instruments, and manages data evaluation.

visionCATS stands for ease of use and intuitive simplicity. The software organizes the workflow of HPTLC, controls the involved CAMAG® HPTLC PRO Modules and CAMAG® instruments, and manages data.

The easy to navigate user interface effectively guides the user through the chromatographic process – from definition of samples and substances to reporting of results. Simply select one of the default methods and start working: fill in the sample table, select a developing solvent and the derivatization reagent.

If necessary modify detection parameters. Then visionCATS will guide you. Creating your own method is easy as well: just select the desired steps. The new sample-oriented approach allows for creating virtual plates from tracks originating from different plates, e.g. for batch-to-batch comparison or long-term stability testing.

With visionCATS relevant samples can be located easier and faster than ever: a powerful search tool within the file explorer that includes extended preview functionalities enables the user to easily search for text and date, samples, methods, and analysis files. Custom search filters can also be created.

Key Features

Comparison Viewer

With the Comparison Viewer images and profiles of tracks originating from the same or different plates and/or different detection modes can be compared on the same screen side by side. Spectra obtained by spectrum scan with the TLC Scanner 4 can be compared as well.

Image Enhancement Tools

visionCATS supports low-noise, high-dynamic-range imaging (HDRI) and includes a comprehensive set of Image Enhancement Tools.

Scanning Densitometry and Spectral Evaluation

Following their chromatographic separation substances on the plate can be measured by Scanning Densitometry. Recording of UV/VIS spectra and their comparison with reference spectra allows identification.

Quantitative Analysis

To determine the substance concentration in a sample after densitometric or image analysis, five different quantification functions (e.g. linear and polynomial regression modes) are available. Several scanning steps and up to five different evaluations can be performed in one analysis file.

HPTLC Method Library

For seamless import of validated methods and images of standards and reference materials, visionCATS provides a free of charge HPTLC Method Library for licensed users.

Regulatory Compliance

visionCATS supports compliance with cGMP/cGLP and 21 CFR Part 11.

State-of-the-art Software Architecture

visionCATS is based on a client/server system, enabling scalability from a single workstation to a multi-user lab network.

HPTLC Analysis - Made Easy

HPTLC Analysis - Made Easy: Create your own method with a few mouse clicks
 

Guided Analysis

Guided Analysis: Select a method and visionCATS will guide you
 

Sample View

Sample View: All available data related to the sample are displayed
 

Data Preview

Data Preview: Provides an overview of steps and samples of an analysis or method
 

Profile Comparison

With visionCATS, it is possible to compare profiles of samples developed in different analyses.
 

Documentation with CAMAG® TLC Visualizer 2

Controlled by visionCATS, the TLC Visualizer 2 can generate low-noise, high-dynamic-range images (HDRI). These images can be separated into tracks representing single samples. Any sample that has ever been analyzed can be directly compared with any other sample using the Comparison Viewer. Samples can be rearranged and compared to reference substances or samples, independent of their plate origin. Also 3D profiles of images can be generated.

The comprehensive set of Image Enhancement Tools in the Data View exploits the TLC Visualizer’s full potential. Images are automatically captured based on an optimized control of the illumination and parameters specified in the HPTLC method. Sophisticated algorithms guarantee the highest image quality for identification of even the weakest zones. The Data View allows visual evaluation of a plate in different illumination modes and offers a broad range of helpful tools, e.g. the RF tool displays the RF value of zones on the digital image, or a rectangular and/or linear zoom function.

Comparison Viewer

Comparison Viewer: Side by side view of tracks from the same or different plates and/or detection modes
 

Image Enhancement

Image Enhancement: Exposure normalization for visualization of weak zones (right) and original image (left)
 

Data View

Data View: All data gathered through the chromatographic process can be reviewed and processed
 

Quantitative evaluation with CAMAG® TLC Scanner 4

visionCATS controls the TLC Scanner 4 and enables quantitative evaluation of the generated densitometric data. The spectral range of light from 190 to 900 nm is available for selecting single wavelengths for Scanning Densitometry. Detection can thus be fine-tuned to match the spectral properties of the analyte to its optimized specificity and sensitivity of the detection. For almost unlimited flexibility, several scanning steps, e.g. before and after derivatization, can be selected. Each scanning step may also include up to 31 individual multi-wavelength scans using different light sources (deuterium lamp, mercury lamp, tungsten lamp). The detection modes absorbance or fluorescence can be combined. The generated data can then be evaluated. Integration of peaks and assignment to separated substances is a matter of a few mouse clicks.

Quantification of substances can be performed via peak height or peak area. The best fitting calibration model with single-level calibration or multilevel calibration via linear, polynomial or Michaelis-Menten regression is selected. With visionCATS and the TLC Scanner 4 UV spectra can be measured as well. The selected peak positions, which are detected from a previous densitogram, are automatically scanned at the defined wavelengths. Spectra can be measured from 190 to 900 nm and displayed individually or overlaid in one diagram. Spectra recorded on different plates can be compared with the Comparison Viewer.

Peak Assignment

Peak Assignment: The separated compounds are assigned. For quantification, data from the multi-wavelength scan at the optimum wavelength for each compound is used.
 

3D View and Peak Integration

3D View and Peak Integration: Densitograms are displayed in 3D, top or front view. Several peak integration and baseline correction settings can be selected.
 

Calibration Curve

Calibration Curve: For evaluation the best fitting calibration model is used. Quantitation can be done via peak height or area.
 

visionCATS Method Library

For analysts using HPTLC for identification of herbal drugs or analysis of food samples, visionCATS offers breakthrough support with the visionCATS Method Library. As a licensed visionCATS user, you can download methods from the visionCATS Method Library free of charge and import them into your own visionCATS database. Each method includes three files:

  • A method documented in a form (e.g. docx) which may serve as an SOP and which can be easily incorporated into any quality management system. This file contains a description of the System Suitability Test (SST) and acceptance criteria for compliant herbal drugs.
  • An instrument method ready to use in visionCATS. A new analysis with all method relevant information (reference substances, plate layout, stationary and mobile phase, etc.) can then be processed. Only the Vial ID of the sample to be analyzed needs to be entered.
  • A Comparison Viewer file with reference images against which each analyzed sample can be compared and evaluated, based on acceptance criteria specified in the method document. After comparing the data of the samples with the reference images individual reports can be generated by a mouse click.

The HPTLC Method Library contains methods included in the USP Dietary Supplement Compendium and those published by the International Association for the Advancement of HPTLC. Numerous methods of identification from the European Pharmacopoeia are added as well. Additional methods will be added continually.

visionCATS Method Library

Compliance

21 CFR Part 11

The option 21 CFR Part 11 supports customers to work in a controlled environment and to establish regulatory-compliant processes. As a part of 21 CFR Part 11 compliance, electronic signature processes are available. A set of log files (System log; Method log; Analysis log; Backup log) are available to fully meet 21 CFR Part 11 requirements.

Qualification

For customers working in a cGMP/cGLP environment, CAMAG offers Installation Qualification (IQ) and Operation Qualification (OQ) services.

System Suitability Test (SST)

System Suitability Tests are used on each plate to check and ensure that the analysis was performed appropriately to produce results that are within the acceptance criteria established during method development. Only data from plates qualified by the respective SST can be directly compared. The SST is the primary tool for evaluation of data during a transfer validation. visionCATS provides several tools for setting up a SST.

Data Security

Data Security is achieved through access control, which is provided by a flexible, secure, and customizable User Management System and basic system settings. Users are defined with rights that determine the level of access they have. The User Management System is compatible with Active Directory. With the powerful user management, it is possible to reflect the customers’ individual safety regulations. Data are stored in a safe database to prevent accidental alteration/deletion.

Data Safety

The visionCATS Backup Assistant helps users to store their data into a backup file. Automatic backup tasks can be defined with the Schedule Assistant. With the Restore Assistant users can easily restore their data from the backup file.

System Requirements

Recommended

  • 3rd gen (Ivy Bridge) Intel Core i5 (i5-3xxx) or equivalent
  • Nvidia GeForce GTX 760 or AMD Radeon R9 270
  • 8 GB RAM
  • Windows 10 Pro (or Enterprise) 64-bit
  • True color 32bits, Full HD (or higher) monitor resolution with a Windows Aero theme enabled
  • 1 TB Hard Drive (frequent user)
  • Mouse with a scroll wheel and 3 buttons

Minimum

  • 3rd gen (Ivy Bridge) Intel Core i3 (i3-3xxx) or equivalent
  • Nvidia GeForce GT 750M or Intel Iris Pro Graphics 580
  • 4 GB RAM Windows 7 Professional 64-bit
  • True color 32bits, 1280 x 1024 monitor resolution with a Windows Aero theme enabled
  • 500 GB Hard Drive (occasional user)
  • Mouse or touchpad with 2 buttons

Downloads

Environment Specifications
pdf
Brochure
pdf
Installation Guide
pdf
Guide to visionCATS
pdf
Download fully functional visionCATS trial version (2.5.18262.1)

The trial version is a full version that expires after 60 days, during which you would need to obtain the full license to extend the use of the software.

zip

Ordering Information

028.0000

CAMAG® HPTLC Software visionCATS Basic Version

including access and control of all instruments - 1 server, 1 client Instrument Diagnostics (xQ), analytical reports - access to method library. Needs to be bought separately and is not included in any Ultimate Package

028.1000

CAMAG® HPTLC Software visionCATS: visionCATS Ultimate

combining: Visualizer Ultimate Package (028.2000), Scanner Ultimate Package (028.3000); CAMAG HPTLC Software visionCATS Basic Version (028.0000) needs to be purchased separately

028.2000

CAMAG® HPTLC Software visionCATS: Visualizer Ultimate Package

combining all CAMAG Visualizer Packages, including: Visualizer Qualitative Package (028.2010), Visualizer Enhanced Evaluation Package (028.2020); CAMAG HPTLC Software visionCATS Basic Version (028.0000) needs to be purchased separately

028.2010

CAMAG® HPTLC Software visionCATS: Visualizer Qualitative Package

028.2020

CAMAG® HPTLC Software visionCATS: Visualizer Enhanced Evaluation Package

028.3000

CAMAG® HPTLC Software visionCATS: Scanner Ultimate Package Multi Wavelength Feature, Spectrum Scanning and Quantification Package; CAMAG HPTLC Software visionCATS Basic Version (028.0000) needs to be purchased separately

028.4000

CAMAG® HPTLC Software visionCATS: Option "21 CFR Part 11"

028.4100

CAMAG® HPTLC Software visionCATS: Option "Export of Data"

028.5000

visionCATS license for a second client

028.5100

visionCATS license for a third, fourth and fifth client, each

028.5200

visionCATS license for a sixth and all subsequent clients, each

Supported Products