Planta med. 65, 408-412 (1999). TLC of sophoricoside on silica gel with chloroform - methanol - water 5:1:1, on RP-18 with methanol - water 1:1, of genistein on silica gel with n-hexane - acetone 2:1, of orobol on silica gel with chloroform - methanol 10:1, and of genistin on silica gel with chloroform - methanol - water 50:10: 1 and on RP-18 with methanol - water 1:1. Detection under UV.

J. Agric. Food Chem. 49, 832-839 (2001). TLC of lutein, anteraxanthin, violaxanthin, neoxanthin on silica gel with petroleum ether (65-95°C) - acetone - dimethylamine 10:4:1.

J. Planar Chromatogr. 17, 95-101 (2004). TLC of 15 flavonoids (flavanone, naringenin, flavone, 3-, 6-, 6’-, 7-hydroxyflavone, 3,6-, 3,7-dihydroxyflavone, morin, chrysin, quercetin, galangin, apigenin, kaempferol) and 4 phenolic acids (o-, p-coumaric acid, caffeic acid, ferulic acid) on silica gel after prewashing with methanol with 11 mobile phases. Chloroform - methanol - 98-100 % formic acid 441:30:235 and n-hexane - ethyl acetate - glacial acetic acid 31:14:5 were the most appropriate eluents. Detection under UV light at 254 and 366 nm or after spraying with 1 % methanolic diphenylboryloxyethylamine and 5 % ethanolic poly(ethylene glycol) 4000, and under UV light at 366 nm after spraying with 1 % ethanolic aluminum chloride solution.

J. Planar Chromatogr. 19, 191-194 (2006). TLC of the flavonoid and triterpenoid soyasaponin content (rutin, vitexine, isovitexine, soyasaponin I and VI as standards) on silica gel in a twin-trough chamber with ethyl acetate - formic acid - acetic acid - water 100:11:11:26 for flavonoids. Detection with diphenylboric acid 2-aminoethylester (natural products reagent) followed by PEG reagent. For soyasaponins chloroform - methanol - water 6:4:1 was used. Detection by spraying with anisaldehyde - sulfuric acid reagent followed by heating at 115 °C.

J. Planar Chromatogr. 21, 355-360 (2008). TLC of flavonoids (myrecetin, rutin, catechin, quercetin, and kaempferol) on silica gel in a horizontal chamber with acetone - chloroform - water 8:2:1. Detection by dipping in natural products reagent followed by dipping in PEG reagent (polyethylene glycol 400 in ethanol). Evaluation under UV 254 and 366 nm.

Rev. Bras. Farmacogn. 23, 802-810 (2013). HPTLC of quercetin (1) and gallic acid (2) in Adiantum capillus-veneris on silica gel with toluene - ethyl acetate - formic acid 5:4:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) and (2) were 53 and 84. Linearity was in the range of 200-1600 ng/zone for both (1) and (2).

leaves by a validated HPTLC–photodensitometry method. Phytochem. Anal. 26, 253-260 (2015). HPTLC of oleacein (1), oleuropein (2) and echinacoside (3) in the leaves of Ligustrum vulgare on silica gel with dichloromethane - methanol - formic acid - water 160:50:8:3. Detection by dipping into anisaldehyde (0.5 %, methanolic) and sulfuric acid (5 %, methanolic), followed by heating at 105 °C for 10 min. Quantitative determination by absorbance measurement at 240 nm and 350 nm. The hRF values of (1) to (3) were 82, 58 and 12, respectively. Linearity was in the range of 625-1875 μg/mL for (1), 100-300 μg/mL for (2) and 180-540 μg/mL for (3). LOD and LOQ were 0.58 and 1.77 μg/mL for (1), 0.20 and 0.61 μg/mL for (2) and 0.32 and 0.97 μg/mL for (3), respectively. Intra-day and inter-day precisions were below 3 % (n=3). Average recoveries varied from 102 to 113 % for (1), 106 and 112 % for (2) and 99 and 101 % for (3), respectively.

seed (Takhmaria). J. Planar Chromatogr. 29, 216-220 (2016). HPTLC of apigenin in the seeds of Ocimum basilicum on silica gel with toluene – acetone – formic acid 5:4:1. Quantitative determination by absorbance measurement at 340 nm. The hRF value of apigenin was 71. Linearity was in the range of 100-600 ng/zone. Intermediate precisions were below 0.5 %. The LOD and LOQ were 4 and 12 ng/zone. Recovery was between 98.5 and 100.6 %.