Planta Medica 82 (15), 1374-1380 (2016). To monitor the subfractionation with methanol on cyclodextrin of a butanol fraction of a methanolic Soxhlet extract of Tamaris Africana shoots, TLC on silica gel with n-butanol – acetic acid – water 12:3:5 and chloroform – methanol – water 70:30:3. Chromatograms were derivatized with Natural Product/PEG reagent and with cerium sulfate sulfuric acid reagent. From the obtained subfractions, flavonoids (three sulfated) and phenolic compounds (ferulic and veratric acid derivatives) could be further isolated.

J. Planar Chromatogr. 30, 205-210 (2017). HPTLC of kaempferol (1), scopoletin (2), and vanillic acid (3) in Euphorbia sororia on silica gel with methylbenzene – ethyl acetate – formic acid 80:30:7 for (1) and (2), and methylbenzene – ethyl acetate – methanol – formic acid 35:15:5:3 for (3). Quantitative determination by absorbance measurement at 330 nm for (1) and (2) and 300 nm for (3). The hRF values for (1) to (3) were 47, 33 and 56, respectively. Linearity was between 530-2680 ng/zone for (1), 112-566 ng/zone for (2) and 1083-5381 ng/zone for (3). LOD and LOQ were 17 and 32 ng/zone for (1), 13 and 50 ng/zone for (2) and 10 and 103 ng/zone for (3), respectively. The intermediate precision was below 3 % (n=3). Average recovery rate was 99.3 % for (1), 100.0 % for (2) and 98.1 % for (3).

fruits. J. Planar Chromatogr. 31, 207-212 (2018). HPTLC of gallic acid (1), protocatechuic acid (2) and quercetin (3) in the fruits of Averrhoa carambola on silica gel with toluene – ethyl acetate – formic acid 5:4:1. Quantitative determination by absorbance measurement at 310 nm. The hRf values for (1) to (3) were 29, 37 and 42, respectiely. Linearity was in the range of 100-600 ng/zone for (1) to (3). The intermediate precision was below 2 % (n=3). The LOD and LOQ were 30 and 90 ng/zone for (1), 33 and 100 ng/zone for (2) and 28 and 85 ng/zone for (3), respectively. Recovery ranged between 97.6 and 99.2 % for (1) to (3).

Planta Medica 83(14/15), 1176-1183 (2017). The fractionation on cyclodextrane with methanol – water 9:1 of the acetone macerate of Crocus sativus flowers (without stigma) was analyzed by TLC on silica gel with ethyl acetate – acetic acid – formic acid – water 100:11:11:26. Detection under UV light and after derivatization with natural products reagent (1 % in methanol), followed by 5 % ethanolic PEG solution. The 182 fractions were pooled into 8 fractions, whereby fraction 2 contained pure kaempferol-3-O-sophoroside (its structure and 98 % purity were confirmed by NMR). The yield was 1.4 % of the dried flowers._x000D_

Phytochemistry 25, 1431-1436 (1986). HPTLC of proanthocyanidins and oligomeric flavonoid glycosides on silica with toluene - acetone 2:1, toluene - acetone - water 20:40:1 and 10:20:1 and with ethyl acetate - iso-propanol - water 10:7:1. Detection with 1 % FeCl3 solution. HPTLC separation of closely related flavonoids.

Biochemical Systematics and Ecology 15, 571-573 (1987). Identification of flavonoids by two-dimensional TLC on polyamide with water - n-butanol - acetone - dioxane 70:15:10:5 (first direction) and benzene - MEK - methanol - water 55:23:20:2 (second direction). Examination under UV 366 nm before and after spraying with aminoethyl diphenyl-borate.

Biochem. J. 248, 953-956 (1987). TLC of the metabolites of human faecal bacteria, flavonoid glycosides, aglycones on silica with benzene - acetic acid - water 83:48:2. Detection under UV and by exposing to iodine vapor.

Biochemical Systematics and Ecology 17, 239-247 (1989). TLC of flavonoid aglycones on polyamide with toluene - petrol ether (100-140°C) - MEK - methanol 50:26:11:13 containing traces of butanol. Visualization under UV.