Cumulative CAMAG Bibliography Service CCBS

Our CCBS database includes more than 11,000 abstracts of publications. Perform your own detailed search of TLC/HPTLC literature and find relevant information.

The Cumulative CAMAG Bibliography Service CCBS contains all abstracts of CBS issues beginning with CBS 51. The database is updated after the publication of every other CBS edition. Currently the Cumulative CAMAG Bibliography Service includes more than 11'000 abstracts of publications between 1983 and today. With the online version you can perform your own detailed TLC/HPTLC literature search:

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      130 149
      HPTLC/HPLC-mass spectrometry identification and NMR characterization of major flavonoids of wild lemongrass (Cymbopogon giganteus) collected in Burkina Faso
      R.K. BATIONO*, C.M. DABIRÉ, A. HEMA, R.H.CH. NÉBIÉ, E. PALÉ, M. NACRO (*Laboratory of Applied Organic Chemistry and Physics, Joseph Ki-Zerbo University, Ouagadougou, Burkina Faso; kindanloun@gmail.com)

      Heliyon 8(8), e10103 (2022). Samples were a methanolic extract of Cymbopogon giganteus leaves (= C. caesius subsp. giganteus, Poaceae), as well as flavones as standards: isorhamnetin, luteolin and orientin (=luteolin 8-C-glucoside). HPTLC on silica gel with ethyl acetate – acetic acid – formic acid – water 100:11:11:26. Derivatization for flavones with Neu’s reagent (ethanolamine diphenylborate – PEG). Visualization under UV 365 nm. The standards (hRF 75, 70-72 and 96, respectively) were not detected in the extract. Some analytes detected by the reagent were scraped from the underivatized plate into a tube, and injected through a TLC-MS interface into a double-quadrupole – time-of-flight MS (electrospray ionization). Full mass scan spectra were recorded in positive and negative ionization modes in m/z range 150–550. For 3 of the compounds, isolated through MPLC columns, the HPTLC-MS results, combined to the NMR and HPLC-MS analyses, allowed the identification as epicatechin (hRF 86, a flavanol, not coloured by Neu’s reagent) and as luteolin 8-C- and 6-C-glucosides (hRF 67-70).

      Classification: 4e, 8a, 32e
      130 036
      Propolis from Nariño: Physicochemical properties and biological activity of Propolis
      G. SALAMANCA*, M. OSORIO, J. CABRERA (* Department of Chemistry, University of Tolima, Ibague, Colombia, gsalaman@ut.edu.co)

      Biotecnologia en el Sector Agropecuario y Agroindustrial. 20, 152-154 (2022). HPTLC of propolis on silica gel with toluene - ethyl acetate - formic acid 30:12:5. Detection under UV light at 254 and 366 nm. The method allowed the identification of apigenin, naringenin, quercetin, caffeic acid, galangin, feruloyl and derivatives of coumaric acid.

      Classification: 8a
      130 041
      Bioactivity-directed isolation, characterization, and quantification of an anxiolytic flavonoid from Brassica oleracea L.
      D. KAUR*, R. SHRI, A. KAMBOJ (*Department of Pharmacognosy, G.H.G
      Khalsa College of Pharmacy, Gurusar Sadhar, Ludhiana, India)

      J. Food. Biochem. 45, e13608 (2022). HPTLC of kaempferol-3-O-β-D-glucoside in florets of Brassica oleracea on silica gel with toluene: acetone: formic acid 15:5:1. Quantitative determination by absorbance measurement at 240 nm. The hRF value for kaempferol-3-O-β-D-glucoside was 40.

      Classification: 8a
      130 042
      Antioxidant-rich fraction of Amomum subulatum fruits mitigates experimental methotrexate-induced oxidative stress by regulating TNF-α, IL-1β, and IL-6 proinflammatory cytokines
      S. Drishya , S. Sulekha Dhanisha, Ch. Guruvayoorappan* (*Laboratory of Immunopharmacology and Experimental Therapeutics, Division of Cancer Research, Regional Cancer Centre, Medical College Campus (Research Centre, University of Kerala), Thiruvananthapuram, Kerala, India, gururcctvm@gmail.com)

      J. Food. Biochem. 46, e13855 (2022). HPTLC of Amomum subulatum dry fruits on silica gel with toluene - ethyl acetate - methanol - formic acid 14:6:2:1. Qualitative identification under UV light at 254 and 366 nm. 

      Classification: 8a
      130 044
      Analysis of polyphenols in Aegle marmelos leaf and ameliorative efficacy against diabetic mice through restoration of antioxidant and anti-inflammatory status
      M. IBRAHIM, B. PARVEEM, S. ZAHIRUDDIN, G. GAUTAM, R. PARVEEN, M. KHAN, A. GUPTA, S. AHMAD (*Sayeed Ahmad, Bioactive Natural Product
      Laboratory, SPER, Jamia Hamdard, New Delhi 110062, India, sahmad_jh@yahoo.co.in)

      J. Food. Biochem. 46, e13852 (2022). HPTLC of gallic acid (1), caffeic acid (2), quercetin (3) and ferulic acid (4) in the leaves of Aegle marmelos on silica gel with toluene - ethyl acetate - formic acid 9:10:1. Quantitative determination by absorbance measurement at 254 nm. The hRF values for (1) to (4) were 19, 37, 45 and 48, respectively. The LOD and LOQ were 9 and 24 ng/zone for (1), 6 and 17 ng/zone for (2), 8 and 23 ng/zone for (3) and 6 and 17 ng/zone for (4). Recovery was in the range of 97.9-101.3 % for (1), 100.6-104.0 % for (2), 99.2-110.4 % for (3) and 98.0-99.9 % for (4).

      Classification: 8a
      130 058
      Standardization and validation of phytometabolites by UHPLC and high-performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul
      A. BALKRISHNA, M. TOMER, M. JOSHI, S. GUJRAL, R. MISHRA, J. SRIVASTAVA, A. VARSHNEY* (*Anurag Varshney, Drug Discovery and Development Division, Patanjali Research Institute, NH-58, Haridwar 249 405, Uttarakhand, India, anurag@prft.co.in)

      J. Sep. Sci. 45, 1616-1635 (2022). HPTLC of gallic acid (1), protocatechuic acid (2), vanillic acid (3), cinnamic acid (4), piperine (5), guggulsterone-E (6), and guggulsterone-Z (7) in Mahayograj Guggul on silica gel with toluene - ethyl acetate - formic acid 10:9:2 for (1) to (3) and toluene - acetone 9:1 for (4) to (7). Quantitative determination by absorbance measurement at 250 nm for (6) and (7), 280 nm for (1), (2), (3) and (4) and 343 nm for (5). The hRF values for (1) to (7) were 30, 41, 47, 15, 33, 41 and 45, respectively. Linearity was between 100 and 1000 µg/mL for (1), 5 and 60 µg/mL for (2), 10 and 80 µg/mL for (3), (4) and (7), 20 and 100 µg/mL for (5) and 40 and 120 µg/mL for (6). Inter-day and intra-day precisions were below 4 % (n=18). The LOD and LOQ were 4 and 14 µg/g for (1), 7 and 21 µg/g for (2), 24 and 72 µg/g for (3), 0.8 and 2.4 µg/g for (4), 12 and 35 µg/g for (5), 2 and 6 µg/g for (6) and 4 and 14 µg/g for (7), respectively. Recovery was between 86.6 and 102.0 % for (1) to (7).

      Classification: 8a, 13c
      130 063
      Comparative evaluation of phenolic profile and antioxidant activity of new sweet cherry (Prunus avium L.) genotypes in Turkey
      M. OCKUN*, Y. GERCEK, H. DEMIRSOY, L. DEMIRSOY, I. MACIT, G. OZ (*Mehmet Ali Ockun, Department of Biology, Botany Division, Istanbul University, Istanbul, Turkey, mehmetaliockun@hotmail.com)

      Phytochem. Anal. 33, 564-576 (2022). HPTLC of flavonoids (1), anthocyanin (2) and antioxidant structures in sweet cherry (Prunus avium) on silica gel with ethyl acetate - dichloromethane - formic acid - acetic acid - water 100:25:10:10:11 for (1) and ethyl acetate - formic acid - acetic acid - water 100:11:11:26 for (2). Detection by spraying with natural product reagent/polyethylene glycol 400 (NP/PEG) solution or 0.1 % methanolic DPPH solution. Qualitative analysis under UV light at 366 nm. 

      Classification: 8a
      130 025
      Hepatoprotective effect of ethanolic extract of Trichosanthes lobata on paracetamol-induced liver toxicity in rats
      A. RAJASEKARAN*, M. PERIYASAMY (Department of Pharmaceutical Chemistry, KMCH College of Pharmacy, Coimbatore, Tamilnadu, India; *rsekaran2001in@yahoo.co.in)

      Chinese Medicine 7, 12 (2012). TLC of a Soxhlet hydro-ethanolic extract of Trichosanthes lobata leaves (Cucurbitaceae) on silica gel with n-hexane – ethyl acetate 7:3. Derivatization with anisaldehyde – sulfuric acid reagent. The presence of flavonoids, saponins, and tannins was found.

      Classification: 8a, 8b, 14, 32e