CAMAG® Derivatizer

    Automated spraying device for reagent transfer onto TLC/HPTLC plates

CAMAG® Derivatizer

The Derivatizer is used for automated reagent transfer in the derivatization of thin-layer chromatograms and sets a new standard of reproducibility by employing a unique “micro droplet” spraying technology (patented). The Derivatizer ensures homogeneity and convenience in applying derivatization reagents, and offers other advantages as compared to manual spraying and immersion. The device is suitable for all common reagents. To meet the divergent physicochemical properties of the reagents, e.g. viscosity, four different color-coded nozzles are available, and the user can select from six spraying modes.

Key Features

  • Unsurpassed homogeneous reagent distribution
  • Environmentally friendly and safe handling through a closed system
  • Reproducible and user-independent results
  • Low reagent consumption (2-4 mL)
  • Hood for 20 x 10 cm and/or 20 x 20 cm plates
  • Intuitive handling and easy cleaning
Button is pushed

At the push of a button, the transparent hood automatically lifts so that the user can remove the plate tray and place the TLC/HPTLC plate on it.

Sealed hood

After the tray is inserted into its original operating position, the hood is lowered and completely sealed to prevent aerosols from leaking to the outside.

Reagent is filled into the nozzle

The user fills the required reagent volume into the appropriate nozzle, selects the optimum spraying level and starts the reagent transfer.

Nozzle generates extremely reagent aerosol

The nozzle generates an extremely fine reagent aerosol, which evenly distributes in the chamber and gradually settles down on the TLC/HPTLC plate.

Wash bottle

Any residue remaining in the gas phase is automatically aspirated by the integrated pump and collected in the wash bottle behind the instrument.

Plate is removed

After the reagent transfer has been completed, the hood lifts at the push of a button and the TLC/HPTLC plate can be removed for further processing.

Derivatization means another step in the process, consequently causing an increase in variance. Even if an experienced technician manually sprays a reagent, the relative standard deviation (RSD) of the measured values rises up to 12%. Using the Derivatizer, the RSD increases by less than 5%, thus providing very reproducible results, equal to those obtained with the Chromatogram Immersion Device, yet consuming considerably less reagent.

The following most common reagents have been tested and validated by the CAMAG laboratory for use with the Derivatizer:

  • Sulfuric acid reagent (10 % in methanol)
  • Anisaldehyde reagent
  • Natural product reagent
  • Polyethylene glycol solution
  • Iodine solution (0.5% in ethanol)
  • Dragendorff reagent
  • Fast blue salt B reagent
  • Ehrlich’s reagent
  • Phosphomolybdic acid reagent
  • Ninhydrin reagent Copper (II) sulfate reagent
  • Aniline-diphenylamine-phosphoric acid reagent
  • Vanillin reagent
  • Potassium hydroxide solution (5% in methanol)
  • Aqueous solutions (enzymatic solutions, etc.)

The Derivatizer is available for two different plate formats (20 x 20 cm and 20 x 10 cm).

For alternative spraying reagents and reagents that are problematic to spray, please download the recommendation list.

Downloads

Brochure Derivatizer
pdf
Reagent List Derivatizer
pdf
Instruction Manual Derivatizer
pdf
Declaration of Conformity Derivatizer
pdf
Preparation, reagent transfer and cleaning instructions for Derivatizer
pdf

Technical Specifications

Plate/sheet size supported

Optimized for two plate formats 20 x 20 cm and 20 x 10 cm

Plate/sheet material supported

Glass, aluminium and plastic

Derivatization method

Automatic spraying using unique “micro droplet“ technology

Reagent consumption

  • 4 ml for 20 x 20 cm plates
  • 2–3 ml for 20 x 10 cm plates

Instrument operation and control

Via nine illuminated buttons (stand-alone instrument)

Operating temperature

10–40°C

Power connection

100–240 VAC, 50–60 Hz, 30 W

Dimensions (W x D x H)

245 x 430 x 355 mm

Weight

12 kg

Ordering Information

022.6000

CAMAG® Derivatizer

complete set consisting of:

022.6030 CAMAG® Derivatizer Base Unit for the automated derivatizaton of TLC plates up to 20 x 20 cm using "micro droplet" spraying technology, 100–240 V
022.6050 Hood for 20 x 20 cm plates
022.6051 Tray for 20 x 20 cm plates
022.6055 Hood for 20 x 10 cm plates
022.6056 Tray for 20 x 10 cm plates
022.6061 Nozzle green
022.6062 Nozzle blue
022.6063 Nozzle yellow
022.6064 Nozzle red
022.6070 Tray Sealing for 20 x 20 cm plates (Pack of 5)
022.6071 Tray Sealing for 20 x 10 cm plates (Pack of 5)

022.6020

CAMAG® Derivatizer 20x20

complete set consisting of:

022.6030 CAMAG® Derivatizer Base Unit for the automated derivatizaton of TLC plates up to 20 x 20 cm using "micro droplet" spraying technology, 100–240 V
022.6050 Hood for 20 x 20 cm plates
022.6051 Tray for 20 x 20 cm plates
022.6061 Nozzle green
022.6062 Nozzle blue
022.6063 Nozzle yellow
022.6064 Nozzle red
022.6070 Tray Sealing for 20 x 20 cm plates (Pack of 5)

022.6010

CAMAG® Derivatizer 20x10

complete set consisting of:

022.6030 CAMAG® Derivatizer Base Unit for the automated derivatizaton of TLC plates up to 20 x 20 cm using "micro droplet" spraying technology, 100–240 V
022.6055 Hood for 20 x 10 cm plates
022.6056 Tray for 20 x 10 cm plates
022.6061 Nozzle green
022.6062 Nozzle blue
022.6063 Nozzle yellow
022.6064 Nozzle red
022.6071 Tray Sealing for 20 x 10 cm plates (Pack of 5)

022.6080

Set of all four nozzles (green, blue, yellow, red)

022.6091

Set of three nozzles, green

022.6092

Set of three nozzles, blue

022.6093

Set of three nozzles, yellow

022.6094

Set of three nozzles, red

022.6085

Set of all four nozzles "Ultra" (green, blue, yellow, red)

022.6066

Nozzle "Ultra", green

022.6067

Nozzle "Ultra", blue

022.6068

Nozzle "Ultra", yellow

022.6069

Nozzle "Ultra", red

022.6096

Set of three nozzles "Ultra", green

022.6097

Set of three nozzles "Ultra", blue

022.6098

Set of three nozzles "Ultra", yellow

022.6099

Set of three nozzles "Ultra", red

Background-light