In the 1st HPTLC Online User Meeting 2023, our experts are discussing important advances in planar chromatography that impact analysts working in the dietary supplement industry. Further, they present information for all planar chromatographers to get higher order data from their analytical workflows. These resources are invaluable to food, forensic, pharmaceutical and environmental chemists as well.
Presentation 1: Scanning Densitometry, Additional Visualization, and HPTLC-Mass Spectrometry in the Quality Control of Botanical and Non-Botanical Dietary Supplements
James P. Kababick, Founder and Director of Flora Research Laboratories, LCC
In the US, 21 CFR part 111 regulates dietary supplements, both botanical and non-botanical. High-Performance Thin-Layer Chromatography (HPTLC) is considered the gold standard for botanical identity verification. Additional data layers, available from the same HPTLC plate through scanning densitometry, HPTLC-MS, and other visualization techniques, can provide insights not always obtainable with the fundamental visualization methods outlined in monographs. We will explore how these extra data layers can make a difference and how to incorporate them in your lab.
Presentation 2: High-Performance Thin-Layer Chromatography <203> looking behind the standardized parameters
Wilmer Perera, Ph.D., Laboratory Manager at CAMAG Scientific, Inc.
USP Chapters <203> and <1064> outline a procedure for using HPTLC in identification tests. HPTLC variables for sample application, chromatogram development, visualization, detection, documentation, and evaluation have been optimized and described in the chapters. Practical examples demonstrate that controlling all HPTLC parameters is crucial to achieve reproducibility in identifying botanical articles and effectively implementing USP methods into daily quality control.