J Chromatogr A, 1684, 463582 (2022). Samples were concentrated filtrates of leachates of waste deposition sites, as well as testosterone, flutamide, bisphenol A (BPA) and nitroquinoline oxide (NQO) as standards. Automated Multiple Development on HPTLC silica gel (prewashed with methanol and dried 30 min at 110 °C) with 1) methanol up to 20 mm; 2A) chloroform – ethyl acetate –petroleum ether 11:4:5 or 2B) ethyl acetate – n-hexane 1:1 for flutamide and testosterone, up to 90 mm. Effect-directed analysis was performed by automated spraying 3 mL suspension of BJ1991 yeast (transfected Saccharomyces cerevisiae strain, pure for androgenic activity, with 50 ng/mL testosterone for anti-androgenic assay), followed by 20 h incubation at 30 °C in a closed chamber (90 % relative humidity), by 5 min drying under cold air stream, by spraying 2.5 mL MUG solution (4-methylumbelliferyl-galactopyranoside) and by 15 min incubation at 37 °C in an open chamber. Agonistic and antagonistic activities were detected qualitatively under UV 366 nm (light or dark blue bands, respectively, on blue background) and quantitatively documented using automated scanning at excitation wavelength 320 nm (deuterium lamp), with cut-off filter at 400 nm. Dose-response curves for model compounds were established by regression analysis. Anti-androgenic effective doses at 10 % were 28 ng/zone for flutamide and 20 ng/zone for BPA, without toxicity for the yeast. To exclude cytotoxicity where anti-androgenic activity was observed, the HPTLC layers (either without or after the spraying with MUG) were sprayed with 3 mL resazurin solution (0.01 % in water) and incubated 30 min at 30 °C and 90 % humidity. Cytotoxicity bands appeared as pink zones of resorufin on a colorless background (dihydroresorufin) under white light. Densitometric evaluation in absorption mode at 575 nm (under deuterium and halogen-tungsten lamps, no filter applied). NQO was cytotoxic at its lowest tested dose (1 ng/zone).

Anal. Chim. Acta 1174 (2021) 3387022. Presentation of the next generation LabToGo system OCLab2 which was improved and made more robust e.g. by using print and media technologies in chromatography, so-called office chromatography (OC). The first LabToGo methods were developed in the field of food safety and quality for point-of-care or on-site usage. For application volume study,  HPTLC of different methanolic / propanolic Patent Blue V solutions on HPTLC silica gel with ethyl acetate - methanol - water - acetic acid 65:23:11:1 up to 50 mm. Evaluation in white light and  absorption measurement at 635 nm. For performance tests of plate imaging, HPTLC of benzoic acid and sorbic acid acid on HPTLC with ethyl acetate - toluene - acetic acid 25:25:1. For study of the illumination homogeneity, HPTLC of either sorbic acid, 7-hydroxy-4-methylcoumarin, or Patent Blue V on amino phase, evaluation at 254 nm (sorbic acid), 366 nm (7-hydroxy-4-methylcoumarin) and in white light (Patent Blue V), and in the OCLab2 at 278 nm, 365 nm, and white light, respectively, and scanning the plates at 254 nm 366 nm and 635 nm, respectively. For analysis of food dye mixture by OCLab2, HPTLC on silica gel with ethyl acetate - methanol - water - acetic acid 65:23:11:1 up to 5 cm, drying and capturing of plate images by the OCLab2 at 515-525 nm, 620-630 nm and 465-475 nm, video densitometry and evaluation using the open-source quanTLC software. For analysis of Stevia leave samples, the ethanolic extracts and four calibration levels of a steviol glycoside mixture were applied by OCLab2 for the hydrophilic interaction liquid chromatography (HILIC) separation on HPTLC silica gel with acetonitrile - water 5:1. Derivatization with 2-naphthol sulfuric acid reagent at evaluation in white light. For analysis of water and environmental samples, ink-printing the prepared samples on HPTLC silica gel MS-grade, focusing using acetone - water 4:1 up to 13 mm, drying,  developing with ethyl acetate - toluene 1:1 up to 70 mm, drying and capturing the plate images in white light, 254 nm and 366 nm. For biological detection, spraying the plate with A. fischeri bacteria suspension and capturing of images over 36 min with an exposure time of 60 s and a trigger interval of 3.0 min. 

J. Chromatogr. 437, 328-332 (1988). Preparative separation of closely related alkaloids from Papaver and Fumaria species on aluminium oxide by centrifugal TLC with cyclohexane - toluene - diethylamine 320:80:1, 160:40:1 or 160:40:3. Flow-rate 0.5-1.5 mL/min. The rotors (1 mm layer thickness) were prepared from a mixture of 40 g basic aluminium oxide, 10g calcium sulfate hemihydrate and 110 mL water.

J. Planar Chromatogr. 2, 53-61 (1989). Construction of the new instrument with the principle of five rotation planar chromatographic methods. The efficiency of the RPC methods demonstrated with the separation of saponin glycosides from Panax ginseng and some furocoumarin isomers from Pastinaca sativa. For preparative separation, an extract from Peucedanum palustre was chosen.

J. Chromatogr. 518, 273-276 (1990). TLC of Chelidonium alkaloids on silica by stepwise gradient with binary and ternary eluents comprising toluene, methanol, ethyl acetate, isopropanol. Detection under UV 366 nm, or by spraying with Dragendorff’s reagent.

J. Planar Chromatogr. 4, 345-359 (1991). Description of the evolution of automated multiple development from manual multiple development through programmed multiple development and discussion of the general role of solvent strength gradients and solvent selectivity emphasizing their application to AMD. The large number of experimental parameters encountered in method development is recognized as one of the main current weaknesses of AMD. The method has, however, a number of attractive features which will ensure continuing interest in the technique.

Anal. Chem. 65, 588-595 (1993). Application of planar chromatography with forced flow development to characterize the kinetic and some thermodynamic properties of octadecylsilanized silica supported by membranes intended for use in solid-phase extraction. Separation of two test mixtures on the membranes with methanol.

Multi-stage development over a constant distance. J. Chromatogr. A 726, 185-192 (1996). Presentation of a theoretical model of multiple development over a constant distance, which is verified with experimental results. Calculation of the final Rf values by computer program for known retention vs. eluent composition relationships for the solutes. Comparison of the predicted and experimental Rf values.